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Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor

Descurainia sophia seeds (DS), Astragalus mongholicus (AM), and their formulas are widely used to treat heart failure caused by various cardiac diseases in traditional Chinese medicine practice. However, the molecular mechanism of action of DS and AM has not been completely understood. Herein, we fi...

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Autores principales: Liu, Xingkai, Chen, Qian, Ji, Xuming, Yu, Wanchen, Wang, Tong, Han, Juanjuan, Li, Shumu, Liu, Jianan, Zeng, Fangang, Zhao, Yao, Zhang, Yanyan, Luo, Qun, Wang, Shijun, Wang, Fuyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403516/
https://www.ncbi.nlm.nih.gov/pubmed/36034815
http://dx.doi.org/10.3389/fphar.2022.939483
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author Liu, Xingkai
Chen, Qian
Ji, Xuming
Yu, Wanchen
Wang, Tong
Han, Juanjuan
Li, Shumu
Liu, Jianan
Zeng, Fangang
Zhao, Yao
Zhang, Yanyan
Luo, Qun
Wang, Shijun
Wang, Fuyi
author_facet Liu, Xingkai
Chen, Qian
Ji, Xuming
Yu, Wanchen
Wang, Tong
Han, Juanjuan
Li, Shumu
Liu, Jianan
Zeng, Fangang
Zhao, Yao
Zhang, Yanyan
Luo, Qun
Wang, Shijun
Wang, Fuyi
author_sort Liu, Xingkai
collection PubMed
description Descurainia sophia seeds (DS), Astragalus mongholicus (AM), and their formulas are widely used to treat heart failure caused by various cardiac diseases in traditional Chinese medicine practice. However, the molecular mechanism of action of DS and AM has not been completely understood. Herein, we first used mass spectrometry coupled to UPLC to characterize the chemical components of DS and AM decoctions, then applied MS-based quantitative proteomic analysis to profile protein expression in the heart of rats with isoproterenol-induced cardiomyopathy (ISO-iCM) before and after treated with DS alone or combined with AM, astragaloside IV (AS4), calycosin-7-glucoside (C7G), and Astragalus polysaccharides (APS) from AM. We demonstrated for the first time that DS decoction alone could reverse the most of differentially expressed proteins in the heart of the rats with ISO-iCM, including the commonly recognized biomarkers natriuretic peptides (NPPA) of cardiomyopathy and sarcomeric myosin light chain 4 (MYL4), relieving ISO-iCM in rats, but AM did not pronouncedly improve the pharmacological efficiency of DS. Significantly, we revealed that AS4 remarkably promoted the pharmacological potency of DS by complementarily reversing myosin motor MYH6/7, and further downregulating NPPA and MYL4. In contrast, APS reduced the efficiency of DS due to upregulating NPPA and MYL4. These findings not only provide novel insights to better understanding in the combination principle of traditional Chinese medicine but also highlight the power of mass spectrometric proteomics strategy combined with conventional pathological approaches for the traditional medicine research.
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spelling pubmed-94035162022-08-26 Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor Liu, Xingkai Chen, Qian Ji, Xuming Yu, Wanchen Wang, Tong Han, Juanjuan Li, Shumu Liu, Jianan Zeng, Fangang Zhao, Yao Zhang, Yanyan Luo, Qun Wang, Shijun Wang, Fuyi Front Pharmacol Pharmacology Descurainia sophia seeds (DS), Astragalus mongholicus (AM), and their formulas are widely used to treat heart failure caused by various cardiac diseases in traditional Chinese medicine practice. However, the molecular mechanism of action of DS and AM has not been completely understood. Herein, we first used mass spectrometry coupled to UPLC to characterize the chemical components of DS and AM decoctions, then applied MS-based quantitative proteomic analysis to profile protein expression in the heart of rats with isoproterenol-induced cardiomyopathy (ISO-iCM) before and after treated with DS alone or combined with AM, astragaloside IV (AS4), calycosin-7-glucoside (C7G), and Astragalus polysaccharides (APS) from AM. We demonstrated for the first time that DS decoction alone could reverse the most of differentially expressed proteins in the heart of the rats with ISO-iCM, including the commonly recognized biomarkers natriuretic peptides (NPPA) of cardiomyopathy and sarcomeric myosin light chain 4 (MYL4), relieving ISO-iCM in rats, but AM did not pronouncedly improve the pharmacological efficiency of DS. Significantly, we revealed that AS4 remarkably promoted the pharmacological potency of DS by complementarily reversing myosin motor MYH6/7, and further downregulating NPPA and MYL4. In contrast, APS reduced the efficiency of DS due to upregulating NPPA and MYL4. These findings not only provide novel insights to better understanding in the combination principle of traditional Chinese medicine but also highlight the power of mass spectrometric proteomics strategy combined with conventional pathological approaches for the traditional medicine research. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9403516/ /pubmed/36034815 http://dx.doi.org/10.3389/fphar.2022.939483 Text en Copyright © 2022 Liu, Chen, Ji, Yu, Wang, Han, Li, Liu, Zeng, Zhao, Zhang, Luo, Wang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Liu, Xingkai
Chen, Qian
Ji, Xuming
Yu, Wanchen
Wang, Tong
Han, Juanjuan
Li, Shumu
Liu, Jianan
Zeng, Fangang
Zhao, Yao
Zhang, Yanyan
Luo, Qun
Wang, Shijun
Wang, Fuyi
Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
title Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
title_full Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
title_fullStr Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
title_full_unstemmed Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
title_short Astragaloside IV promotes pharmacological effect of Descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
title_sort astragaloside iv promotes pharmacological effect of descurainia sophia seeds on isoproterenol-induced cardiomyopathy in rats by synergistically modulating the myosin motor
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403516/
https://www.ncbi.nlm.nih.gov/pubmed/36034815
http://dx.doi.org/10.3389/fphar.2022.939483
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