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Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein

eIF5-mimic protein (5MP) controls translation through its interaction with eukaryotic translation initiation factor (eIF) 2 and eIF3 and alters non-AUG translation rates for oncogenes in cancer and repeat expansions in neurodegenerative disease. To precisely evaluate the effect of 5MP mutations on b...

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Detalles Bibliográficos
Autores principales:	Singh, Chingakham Ranjit, Jaiswal, Rahul, Escalante, Carlos R., Asano, Katsura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403556/ https://www.ncbi.nlm.nih.gov/pubmed/36035794 http://dx.doi.org/10.1016/j.xpro.2022.101615

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