CRISPR-Cas9-directed gene tagging using a single integrase-defective lentiviral vector carrying a transposase-based Cas9 off switch

Locus-directed DNA cleavage induced by the CRISPR-Cas9 system triggers DNA repair mechanisms allowing gene repair or targeted insertion of foreign DNA. For gene insertion to be successful, availability of a homologous donor template needs to be timed with cleavage of the DNA by the Cas9 endonuclease...

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Detalles Bibliográficos
Autores principales: Thomsen, Emil Aagaard, Skipper, Kristian Alsbjerg, Andersen, Sofie, Haslund, Didde, Skov, Thomas Wisbech, Mikkelsen, Jacob Giehm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403905/
https://www.ncbi.nlm.nih.gov/pubmed/36090759
http://dx.doi.org/10.1016/j.omtn.2022.08.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403905/
https://www.ncbi.nlm.nih.gov/pubmed/36090759
http://dx.doi.org/10.1016/j.omtn.2022.08.005

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