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超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药

Fingerprints contain important information such as the ingredients ingested by the donor. By analyzing the characteristic components in fingerprints, the donor can be characterized, which would provide insights for investigation of a given case. This approach can also be used in the qualitative moni...

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Autores principales: DU, Qiuyao, ZHANG, Yunfeng, WANG, Jifen, ZHAO, Peng, WU, Xiaojun, DONG, Linpei, LI, Jiayi, LIU, Bingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial board of Chinese Journal of Chromatography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404012/
https://www.ncbi.nlm.nih.gov/pubmed/35080165
http://dx.doi.org/10.3724/SP.J.1123.2021.05012
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author DU, Qiuyao
ZHANG, Yunfeng
WANG, Jifen
ZHAO, Peng
WU, Xiaojun
DONG, Linpei
LI, Jiayi
LIU, Bingjie
author_facet DU, Qiuyao
ZHANG, Yunfeng
WANG, Jifen
ZHAO, Peng
WU, Xiaojun
DONG, Linpei
LI, Jiayi
LIU, Bingjie
author_sort DU, Qiuyao
collection PubMed
description Fingerprints contain important information such as the ingredients ingested by the donor. By analyzing the characteristic components in fingerprints, the donor can be characterized, which would provide insights for investigation of a given case. This approach can also be used in the qualitative monitoring of drug intake. Therefore, the examination of hypotensive drugs in fingerprints has significant value in practical application. This study established a method based on ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry (UPLC-Q-TRAP/MS) for the simultaneous determination of 36 hypotensive drugs in fingerprints. The pre-treatment method was based on protein precipitation. A 3×3 cm filter paper was cut into pieces and placed in a 2 mL plastic centrifuge tube after fingerprint collection. Then, 0.50 mL methanol was added, followed by vortex mixing for 1 min and ultrasonic oscillation for 3 min. The filter paper was centrifuged at 12000 r/min for 5 min, and the supernatant was withdrawn for sample analysis. An ACQUITY UPLC(®) BEH C18 chromatographic column (100 mm×3.0 mm, 1.7 μm) was selected, with 0.01% aqueous formic acid and methanol as mobile phases for gradient elution. MS analysis involved scheduled multiple reaction monitoring-information dependent acquisition-enhanced product ion (SMRM-IDA-EPI) scanning. This method could be used to retrieve library researching during high-sensitivity analysis, which could increase the accuracy of qualitative results. The calibration curves showed good linearity in the range of 0.05-50.00 ng/fingerprint, with correlation coefficients (r) greater than 0.99 for all 36 analytes. The limits of detection and limits of quantification of the 36 hypotensive drugs were 0.001-0.045 ng/fingerprint and 0.002-0.050 ng/fingerprint, respectively. At spiked levels of 0.25, 2.50, 25.00 ng/fingerprint, the matrix effects, recoveries, intra-day precisions, and inter-day precisions of the 36 hypotensive drugs were 79.0%-119.2%, 79.3%-116.2%, 0.2%-18.3%, and 1.6%-19.1%, respectively. This method was used to detect hypotensive drugs in the fingerprints of 87 hypertensive patients, and hypotensive drug intakes were accurately detected in most cases. The established method is operationally simple, with high sensitivity and good selectivity, and it is suitable for screening and testing hypotensive drugs in fingerprints.
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spelling pubmed-94040122022-09-14 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药 DU, Qiuyao ZHANG, Yunfeng WANG, Jifen ZHAO, Peng WU, Xiaojun DONG, Linpei LI, Jiayi LIU, Bingjie Se Pu Articles Fingerprints contain important information such as the ingredients ingested by the donor. By analyzing the characteristic components in fingerprints, the donor can be characterized, which would provide insights for investigation of a given case. This approach can also be used in the qualitative monitoring of drug intake. Therefore, the examination of hypotensive drugs in fingerprints has significant value in practical application. This study established a method based on ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry (UPLC-Q-TRAP/MS) for the simultaneous determination of 36 hypotensive drugs in fingerprints. The pre-treatment method was based on protein precipitation. A 3×3 cm filter paper was cut into pieces and placed in a 2 mL plastic centrifuge tube after fingerprint collection. Then, 0.50 mL methanol was added, followed by vortex mixing for 1 min and ultrasonic oscillation for 3 min. The filter paper was centrifuged at 12000 r/min for 5 min, and the supernatant was withdrawn for sample analysis. An ACQUITY UPLC(®) BEH C18 chromatographic column (100 mm×3.0 mm, 1.7 μm) was selected, with 0.01% aqueous formic acid and methanol as mobile phases for gradient elution. MS analysis involved scheduled multiple reaction monitoring-information dependent acquisition-enhanced product ion (SMRM-IDA-EPI) scanning. This method could be used to retrieve library researching during high-sensitivity analysis, which could increase the accuracy of qualitative results. The calibration curves showed good linearity in the range of 0.05-50.00 ng/fingerprint, with correlation coefficients (r) greater than 0.99 for all 36 analytes. The limits of detection and limits of quantification of the 36 hypotensive drugs were 0.001-0.045 ng/fingerprint and 0.002-0.050 ng/fingerprint, respectively. At spiked levels of 0.25, 2.50, 25.00 ng/fingerprint, the matrix effects, recoveries, intra-day precisions, and inter-day precisions of the 36 hypotensive drugs were 79.0%-119.2%, 79.3%-116.2%, 0.2%-18.3%, and 1.6%-19.1%, respectively. This method was used to detect hypotensive drugs in the fingerprints of 87 hypertensive patients, and hypotensive drug intakes were accurately detected in most cases. The established method is operationally simple, with high sensitivity and good selectivity, and it is suitable for screening and testing hypotensive drugs in fingerprints. Editorial board of Chinese Journal of Chromatography 2022-02-08 /pmc/articles/PMC9404012/ /pubmed/35080165 http://dx.doi.org/10.3724/SP.J.1123.2021.05012 Text en https://creativecommons.org/licenses/by/4.0/本文是开放获取文章,遵循CC BY 4.0协议 https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
DU, Qiuyao
ZHANG, Yunfeng
WANG, Jifen
ZHAO, Peng
WU, Xiaojun
DONG, Linpei
LI, Jiayi
LIU, Bingjie
超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
title 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
title_full 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
title_fullStr 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
title_full_unstemmed 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
title_short 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
title_sort 超高效液相色谱-三重四极杆复合线性离子阱质谱法同时测定指印中36种降压药
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404012/
https://www.ncbi.nlm.nih.gov/pubmed/35080165
http://dx.doi.org/10.3724/SP.J.1123.2021.05012
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