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高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂
Vecuronium, rocuronium, and pancuronium are widely used as non-depolarizing muscle relaxants. There have been occasional cases of allergic reactions and even death when using such muscle relaxants. Rapid determination of the concentration of these muscle relaxants in blood can provide valuable infor...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial board of Chinese Journal of Chromatography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404065/ https://www.ncbi.nlm.nih.gov/pubmed/34227366 http://dx.doi.org/10.3724/SP.J.1123.2020.09020 |
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author | HUANG, Yongpeng TANG, Hui SONG, Yunyang CHEN, Bo ZHONG, Hui |
author_facet | HUANG, Yongpeng TANG, Hui SONG, Yunyang CHEN, Bo ZHONG, Hui |
author_sort | HUANG, Yongpeng |
collection | PubMed |
description | Vecuronium, rocuronium, and pancuronium are widely used as non-depolarizing muscle relaxants. There have been occasional cases of allergic reactions and even death when using such muscle relaxants. Rapid determination of the concentration of these muscle relaxants in blood can provide valuable information for early clinical diagnosis. As quaternary ammonium compounds, these muscle relaxants are highly polar. Hence, they cannot be retained effectively on reversed-phase chromatographic columns with conventional mobile phases. These quaternary ammonium muscle relaxants are mainly separated by ion-pair chromatography. Using an ion-pairing reagent can help improve the retention capabilities of quaternary ammonium muscle relaxants. Nevertheless, the sensitivity of MS detection is significantly decreased because of ionic inhibition caused by the ion-pairing reagent in the mobile phase. Furthermore, ion-pairing reagents can pollute the MS system. A method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for the simultaneous determination of the three quaternary ammonium muscle relaxants in blood. The blood samples were diluted and subjected to high-speed centrifugation. The supernatant was purified on a Bond Elut AL-N solid phase extraction column and then filtered through a 0.45 μm microporous membrane. The quaternary ammonium muscle relaxants were separated on a ZIC-cHILIC analytical column (50 mm×2.1 mm, 3.0 μm) with gradient elution. Acetonitrile and 0.1% formic acid aqueous solution were used as mobile phases. The separated compounds were analyzed by tandem MS with an electrospray ionization (ESI) source in positive and multiple reaction monitoring (MRM) modes. The matrix effects of vecuronium, rocuronium, and pancuronium in blood were 88.1% to 95.4%. The calibration curves for vecuronium, rocuronium, and pancuronium showed good linear relationships in each range, and all correlation coefficients (R(2)) were > 0.996. The limits of detection of vecuronium, rocuronium, and pancuronium were 0.2-0.8 ng/mL, with the corresponding limits of quantification being 0.5-2.0 ng/mL. The recoveries of vecuronium, rocuronium, and pancuronium were 92.8% to 110.6%, with relative standard deviations (RSDs) of 3.2%-9.4%. This method is sensitive, accurate, and easy to operate, and it can be used to rapidly determine vecuronium, rocuronium, and pancuronium in blood. |
format | Online Article Text |
id | pubmed-9404065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Editorial board of Chinese Journal of Chromatography |
record_format | MEDLINE/PubMed |
spelling | pubmed-94040652022-09-14 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 HUANG, Yongpeng TANG, Hui SONG, Yunyang CHEN, Bo ZHONG, Hui Se Pu Articles Vecuronium, rocuronium, and pancuronium are widely used as non-depolarizing muscle relaxants. There have been occasional cases of allergic reactions and even death when using such muscle relaxants. Rapid determination of the concentration of these muscle relaxants in blood can provide valuable information for early clinical diagnosis. As quaternary ammonium compounds, these muscle relaxants are highly polar. Hence, they cannot be retained effectively on reversed-phase chromatographic columns with conventional mobile phases. These quaternary ammonium muscle relaxants are mainly separated by ion-pair chromatography. Using an ion-pairing reagent can help improve the retention capabilities of quaternary ammonium muscle relaxants. Nevertheless, the sensitivity of MS detection is significantly decreased because of ionic inhibition caused by the ion-pairing reagent in the mobile phase. Furthermore, ion-pairing reagents can pollute the MS system. A method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for the simultaneous determination of the three quaternary ammonium muscle relaxants in blood. The blood samples were diluted and subjected to high-speed centrifugation. The supernatant was purified on a Bond Elut AL-N solid phase extraction column and then filtered through a 0.45 μm microporous membrane. The quaternary ammonium muscle relaxants were separated on a ZIC-cHILIC analytical column (50 mm×2.1 mm, 3.0 μm) with gradient elution. Acetonitrile and 0.1% formic acid aqueous solution were used as mobile phases. The separated compounds were analyzed by tandem MS with an electrospray ionization (ESI) source in positive and multiple reaction monitoring (MRM) modes. The matrix effects of vecuronium, rocuronium, and pancuronium in blood were 88.1% to 95.4%. The calibration curves for vecuronium, rocuronium, and pancuronium showed good linear relationships in each range, and all correlation coefficients (R(2)) were > 0.996. The limits of detection of vecuronium, rocuronium, and pancuronium were 0.2-0.8 ng/mL, with the corresponding limits of quantification being 0.5-2.0 ng/mL. The recoveries of vecuronium, rocuronium, and pancuronium were 92.8% to 110.6%, with relative standard deviations (RSDs) of 3.2%-9.4%. This method is sensitive, accurate, and easy to operate, and it can be used to rapidly determine vecuronium, rocuronium, and pancuronium in blood. Editorial board of Chinese Journal of Chromatography 2021-07-08 /pmc/articles/PMC9404065/ /pubmed/34227366 http://dx.doi.org/10.3724/SP.J.1123.2020.09020 Text en https://creativecommons.org/licenses/by/4.0/本文是开放获取文章,遵循CC BY 4.0协议 https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Articles HUANG, Yongpeng TANG, Hui SONG, Yunyang CHEN, Bo ZHONG, Hui 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
title | 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
title_full | 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
title_fullStr | 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
title_full_unstemmed | 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
title_short | 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
title_sort | 高效液相色谱-串联质谱法同时检测血液中3种季铵盐类肌松剂 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404065/ https://www.ncbi.nlm.nih.gov/pubmed/34227366 http://dx.doi.org/10.3724/SP.J.1123.2020.09020 |
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