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Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients

BACKGROUND: Rheumatoid arthritis (RA) is a chronic autoimmune disease for which prediction of long-term prognosis from disease’s outset is not clinically feasible. The importance of immunoglobulin G (IgG) and its Fc N-glycosylation in inflammation is well-known and studies described its relevance fo...

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Autores principales: Sénard, Thomas, Flouri, Irini, Vučković, Frano, Papadaki, Garyfalia, Goutakoli, Panagiota, Banos, Aggelos, Pučić-Baković, Maja, Pezer, Marija, Bertsias, George, Lauc, Gordan, Sidiropoulos, Prodromos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404591/
https://www.ncbi.nlm.nih.gov/pubmed/36008868
http://dx.doi.org/10.1186/s13075-022-02897-5
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author Sénard, Thomas
Flouri, Irini
Vučković, Frano
Papadaki, Garyfalia
Goutakoli, Panagiota
Banos, Aggelos
Pučić-Baković, Maja
Pezer, Marija
Bertsias, George
Lauc, Gordan
Sidiropoulos, Prodromos
author_facet Sénard, Thomas
Flouri, Irini
Vučković, Frano
Papadaki, Garyfalia
Goutakoli, Panagiota
Banos, Aggelos
Pučić-Baković, Maja
Pezer, Marija
Bertsias, George
Lauc, Gordan
Sidiropoulos, Prodromos
author_sort Sénard, Thomas
collection PubMed
description BACKGROUND: Rheumatoid arthritis (RA) is a chronic autoimmune disease for which prediction of long-term prognosis from disease’s outset is not clinically feasible. The importance of immunoglobulin G (IgG) and its Fc N-glycosylation in inflammation is well-known and studies described its relevance for several autoimmune diseases, including RA. Herein we assessed the association between IgG N-glycoforms and disease prognosis at 2 years in an early inflammatory arthritis cohort. METHODS: Sera from 118 patients with early inflammatory arthritis naïve to treatment sampled at baseline were used to obtain IgG Fc glycopeptides, which were then analyzed in a subclass-specific manner by liquid chromatography coupled to mass spectrometry (LC-MS). Patients were prospectively followed and a favorable prognosis at 2 years was assessed by a combined index as remission or low disease activity (DAS28 < 3.2) and normal functionality (HAQ ≤ 0.25) while on treatment with conventional synthetic DMARDs and never used biologic DMARDs. RESULTS: We observed a significant association between high levels of IgG2/3 Fc galactosylation (effect 0.627 and adjusted p value 0.036 for the fully galactosylated glycoform H5N4F1; effect −0.551 and adjusted p value 0.04963 for the agalactosylated H3N4F1) and favorable outcome after 2 years of treatment. The inclusion of IgG glycoprofiling in a multivariate analysis to predict the outcome (with HAQ, DAS28, RF, and ACPA included in the model) did not improve the prognostic performance of the model. CONCLUSION: Pending confirmation of these findings in larger cohorts, IgG glycosylation levels could be used as a prognostic marker in early arthritis, to overcome the limitations of the current prognostic tools. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13075-022-02897-5.
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spelling pubmed-94045912022-08-26 Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients Sénard, Thomas Flouri, Irini Vučković, Frano Papadaki, Garyfalia Goutakoli, Panagiota Banos, Aggelos Pučić-Baković, Maja Pezer, Marija Bertsias, George Lauc, Gordan Sidiropoulos, Prodromos Arthritis Res Ther Research BACKGROUND: Rheumatoid arthritis (RA) is a chronic autoimmune disease for which prediction of long-term prognosis from disease’s outset is not clinically feasible. The importance of immunoglobulin G (IgG) and its Fc N-glycosylation in inflammation is well-known and studies described its relevance for several autoimmune diseases, including RA. Herein we assessed the association between IgG N-glycoforms and disease prognosis at 2 years in an early inflammatory arthritis cohort. METHODS: Sera from 118 patients with early inflammatory arthritis naïve to treatment sampled at baseline were used to obtain IgG Fc glycopeptides, which were then analyzed in a subclass-specific manner by liquid chromatography coupled to mass spectrometry (LC-MS). Patients were prospectively followed and a favorable prognosis at 2 years was assessed by a combined index as remission or low disease activity (DAS28 < 3.2) and normal functionality (HAQ ≤ 0.25) while on treatment with conventional synthetic DMARDs and never used biologic DMARDs. RESULTS: We observed a significant association between high levels of IgG2/3 Fc galactosylation (effect 0.627 and adjusted p value 0.036 for the fully galactosylated glycoform H5N4F1; effect −0.551 and adjusted p value 0.04963 for the agalactosylated H3N4F1) and favorable outcome after 2 years of treatment. The inclusion of IgG glycoprofiling in a multivariate analysis to predict the outcome (with HAQ, DAS28, RF, and ACPA included in the model) did not improve the prognostic performance of the model. CONCLUSION: Pending confirmation of these findings in larger cohorts, IgG glycosylation levels could be used as a prognostic marker in early arthritis, to overcome the limitations of the current prognostic tools. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13075-022-02897-5. BioMed Central 2022-08-25 2022 /pmc/articles/PMC9404591/ /pubmed/36008868 http://dx.doi.org/10.1186/s13075-022-02897-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sénard, Thomas
Flouri, Irini
Vučković, Frano
Papadaki, Garyfalia
Goutakoli, Panagiota
Banos, Aggelos
Pučić-Baković, Maja
Pezer, Marija
Bertsias, George
Lauc, Gordan
Sidiropoulos, Prodromos
Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
title Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
title_full Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
title_fullStr Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
title_full_unstemmed Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
title_short Baseline IgG-Fc N-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
title_sort baseline igg-fc n-glycosylation profile is associated with long-term outcome in a cohort of early inflammatory arthritis patients
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404591/
https://www.ncbi.nlm.nih.gov/pubmed/36008868
http://dx.doi.org/10.1186/s13075-022-02897-5
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