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A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages

Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, and behavior has been very difficult. Here we used single cell RNAseq to generate a complete gene expression atlas of the Drosophila larval central nervous system composed of 131,077...

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Autores principales: Corrales, Marc, Cocanougher, Benjamin T., Kohn, Andrea B., Wittenbach, Jason D., Long, Xi S., Lemire, Andrew, Cardona, Albert, Singer, Robert H., Moroz, Leonid L., Zlatic, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404646/
https://www.ncbi.nlm.nih.gov/pubmed/36002881
http://dx.doi.org/10.1186/s13064-022-00164-6
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author Corrales, Marc
Cocanougher, Benjamin T.
Kohn, Andrea B.
Wittenbach, Jason D.
Long, Xi S.
Lemire, Andrew
Cardona, Albert
Singer, Robert H.
Moroz, Leonid L.
Zlatic, Marta
author_facet Corrales, Marc
Cocanougher, Benjamin T.
Kohn, Andrea B.
Wittenbach, Jason D.
Long, Xi S.
Lemire, Andrew
Cardona, Albert
Singer, Robert H.
Moroz, Leonid L.
Zlatic, Marta
author_sort Corrales, Marc
collection PubMed
description Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, and behavior has been very difficult. Here we used single cell RNAseq to generate a complete gene expression atlas of the Drosophila larval central nervous system composed of 131,077 single cells across three developmental stages (1 h, 24 h and 48 h after hatching). We identify 67 distinct cell clusters based on the patterns of gene expression. These include 31 functional mature larval neuron clusters, 1 ring gland cluster, 8 glial clusters, 6 neural precursor clusters, and 13 developing immature adult neuron clusters. Some clusters are present across all stages of larval development, while others are stage specific (such as developing adult neurons). We identify genes that are differentially expressed in each cluster, as well as genes that are differentially expressed at distinct stages of larval life. These differentially expressed genes provide promising candidates for regulating the function of specific neuronal and glial types in the larval nervous system, or the specification and differentiation of adult neurons. The cell transcriptome Atlas of the Drosophila larval nervous system is a valuable resource for developmental biology and systems neuroscience and provides a basis for elucidating how genes regulate neural development and function. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13064-022-00164-6.
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spelling pubmed-94046462022-08-26 A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages Corrales, Marc Cocanougher, Benjamin T. Kohn, Andrea B. Wittenbach, Jason D. Long, Xi S. Lemire, Andrew Cardona, Albert Singer, Robert H. Moroz, Leonid L. Zlatic, Marta Neural Dev Research Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, and behavior has been very difficult. Here we used single cell RNAseq to generate a complete gene expression atlas of the Drosophila larval central nervous system composed of 131,077 single cells across three developmental stages (1 h, 24 h and 48 h after hatching). We identify 67 distinct cell clusters based on the patterns of gene expression. These include 31 functional mature larval neuron clusters, 1 ring gland cluster, 8 glial clusters, 6 neural precursor clusters, and 13 developing immature adult neuron clusters. Some clusters are present across all stages of larval development, while others are stage specific (such as developing adult neurons). We identify genes that are differentially expressed in each cluster, as well as genes that are differentially expressed at distinct stages of larval life. These differentially expressed genes provide promising candidates for regulating the function of specific neuronal and glial types in the larval nervous system, or the specification and differentiation of adult neurons. The cell transcriptome Atlas of the Drosophila larval nervous system is a valuable resource for developmental biology and systems neuroscience and provides a basis for elucidating how genes regulate neural development and function. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13064-022-00164-6. BioMed Central 2022-08-24 /pmc/articles/PMC9404646/ /pubmed/36002881 http://dx.doi.org/10.1186/s13064-022-00164-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Corrales, Marc
Cocanougher, Benjamin T.
Kohn, Andrea B.
Wittenbach, Jason D.
Long, Xi S.
Lemire, Andrew
Cardona, Albert
Singer, Robert H.
Moroz, Leonid L.
Zlatic, Marta
A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
title A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
title_full A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
title_fullStr A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
title_full_unstemmed A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
title_short A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
title_sort single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404646/
https://www.ncbi.nlm.nih.gov/pubmed/36002881
http://dx.doi.org/10.1186/s13064-022-00164-6
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