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Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response

Plants coevolved with their antioxidant defense systems, which detoxify and adjust levels of reactive oxygen species (ROS) under multiple plant stresses. We performed whole-genome identification of ascorbate peroxidase (APX) and catalase (CAT) families in cultivated and wild soybeans. In cultivated...

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Autores principales: Aleem, Muqadas, Aleem, Saba, Sharif, Iram, Aleem, Maida, Shahzad, Rahil, Khan, Muhammad Imran, Batool, Amina, Sarwar, Gulam, Farooq, Jehanzeb, Iqbal, Azeem, Jan, Basit Latief, Kaushik, Prashant, Feng, Xianzhong, Bhat, Javaid Akhter, Ahmad, Parvaiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404807/
https://www.ncbi.nlm.nih.gov/pubmed/36009347
http://dx.doi.org/10.3390/antiox11081626
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author Aleem, Muqadas
Aleem, Saba
Sharif, Iram
Aleem, Maida
Shahzad, Rahil
Khan, Muhammad Imran
Batool, Amina
Sarwar, Gulam
Farooq, Jehanzeb
Iqbal, Azeem
Jan, Basit Latief
Kaushik, Prashant
Feng, Xianzhong
Bhat, Javaid Akhter
Ahmad, Parvaiz
author_facet Aleem, Muqadas
Aleem, Saba
Sharif, Iram
Aleem, Maida
Shahzad, Rahil
Khan, Muhammad Imran
Batool, Amina
Sarwar, Gulam
Farooq, Jehanzeb
Iqbal, Azeem
Jan, Basit Latief
Kaushik, Prashant
Feng, Xianzhong
Bhat, Javaid Akhter
Ahmad, Parvaiz
author_sort Aleem, Muqadas
collection PubMed
description Plants coevolved with their antioxidant defense systems, which detoxify and adjust levels of reactive oxygen species (ROS) under multiple plant stresses. We performed whole-genome identification of ascorbate peroxidase (APX) and catalase (CAT) families in cultivated and wild soybeans. In cultivated and wild soybean genomes, we identified 11 and 10 APX genes, respectively, whereas the numbers of identified CAT genes were four in each species. Comparative phylogenetic analysis revealed more homology among cultivated and wild soybeans relative to other legumes. Exon/intron structure, motif and synteny blocks are conserved in cultivated and wild species. According to the Ka/Ks value, purifying selection is a major force for evolution of these gene families in wild soybean; however, the APX gene family was evolved by both positive and purifying selection in cultivated soybean. Segmental duplication was a major factor involved in the expansion of APX and CAT genes. Expression patterns revealed that APX and CAT genes are differentially expressed across fourteen different soybean tissues under water deficit (WD), heat stress (HS) and combined drought plus heat stress (WD + HS). Altogether, the current study provides broad insights into these gene families in soybeans. Our results indicate that APX and CAT gene families modulate multiple stress response in soybeans.
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spelling pubmed-94048072022-08-26 Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response Aleem, Muqadas Aleem, Saba Sharif, Iram Aleem, Maida Shahzad, Rahil Khan, Muhammad Imran Batool, Amina Sarwar, Gulam Farooq, Jehanzeb Iqbal, Azeem Jan, Basit Latief Kaushik, Prashant Feng, Xianzhong Bhat, Javaid Akhter Ahmad, Parvaiz Antioxidants (Basel) Article Plants coevolved with their antioxidant defense systems, which detoxify and adjust levels of reactive oxygen species (ROS) under multiple plant stresses. We performed whole-genome identification of ascorbate peroxidase (APX) and catalase (CAT) families in cultivated and wild soybeans. In cultivated and wild soybean genomes, we identified 11 and 10 APX genes, respectively, whereas the numbers of identified CAT genes were four in each species. Comparative phylogenetic analysis revealed more homology among cultivated and wild soybeans relative to other legumes. Exon/intron structure, motif and synteny blocks are conserved in cultivated and wild species. According to the Ka/Ks value, purifying selection is a major force for evolution of these gene families in wild soybean; however, the APX gene family was evolved by both positive and purifying selection in cultivated soybean. Segmental duplication was a major factor involved in the expansion of APX and CAT genes. Expression patterns revealed that APX and CAT genes are differentially expressed across fourteen different soybean tissues under water deficit (WD), heat stress (HS) and combined drought plus heat stress (WD + HS). Altogether, the current study provides broad insights into these gene families in soybeans. Our results indicate that APX and CAT gene families modulate multiple stress response in soybeans. MDPI 2022-08-22 /pmc/articles/PMC9404807/ /pubmed/36009347 http://dx.doi.org/10.3390/antiox11081626 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aleem, Muqadas
Aleem, Saba
Sharif, Iram
Aleem, Maida
Shahzad, Rahil
Khan, Muhammad Imran
Batool, Amina
Sarwar, Gulam
Farooq, Jehanzeb
Iqbal, Azeem
Jan, Basit Latief
Kaushik, Prashant
Feng, Xianzhong
Bhat, Javaid Akhter
Ahmad, Parvaiz
Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response
title Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response
title_full Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response
title_fullStr Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response
title_full_unstemmed Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response
title_short Whole-Genome Identification of APX and CAT Gene Families in Cultivated and Wild Soybeans and Their Regulatory Function in Plant Development and Stress Response
title_sort whole-genome identification of apx and cat gene families in cultivated and wild soybeans and their regulatory function in plant development and stress response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404807/
https://www.ncbi.nlm.nih.gov/pubmed/36009347
http://dx.doi.org/10.3390/antiox11081626
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