Limited Acclimation in Leaf Morphology and Anatomy to Experimental Drought in Temperate Forest Species

SIMPLE SUMMARY: Climate change shown to have a significant impact on the forest ecosystem due to increased and more frequent occurrence of extreme drought. However, in order to successfully adjust to the xeric environments, plants can usually adopt a variety of adaptation strategies. Here, we investigated the morpho-anatomical traits and biomass allocation patterns as acclimation mechanisms in drought conditions. We found that the interrelation between leaf morphological and anatomical traits were equally affected by drought conditions across all species. This suggests that there is no convincing evidence to classify taxa based on drought resistance vs. drought tolerance. However, based on the biomass allocation pattern, we found that P. koraiensis and F. mandshurica had the higher RMF and total PB, but lower LFM, suggesting higher drought tolerance than those of the other species. Therefore, our dataset revealed some easily measurable traits, such as LMF, RMF, and PB, which demonstrated the seedling’s ability to cope with drought and which could be utilized to choose drought-tolerant species for reforestation in the temperate forest. ABSTRACT: Drought is a critical and increasingly common abiotic factor that has impacts on plant structures and functioning and is a challenge for the successful management of forest ecosystems. Here, we test the shifts in leaf morpho-anatomical or hydraulic traits and plant growth above ground caused by drought. A factorial experiment was conducted with two gymnosperms (Larix gmelinii and Pinus koraiensis) and two angiosperms (Fraxinus mandshurica and Tilia amurensis), tree species grown under three varying drought intensities in NE China. Considering all the species studied, the plant height (PH), root collar diameter (RCD), and plant biomass (PB) were significantly decreased by drought. The leaf thickness (LT) increased, while the leaf area (LA) decreased with drought intensity. In the gymnosperms, the mesophyll thickness (MT) increased, and the resin duct decreased, while in the angiosperms the palisade mesophyll thickness (PMT), the spongy mesophyll thickness (SMT), and the abaxial (ABE) and adaxial epidermis (ADE) thickness were increased by drought. The correlation analysis revealed that P. koraiensis and F. mandshurica had the higher RMF and total plant biomass, but the least LMF, suggesting drought tolerance. In contrast, the L. gmelinii had the least RMF and higher LMF, suggesting vulnerability to drought. Similarly, T. amurensis had the higher leaf size, which increased the evaporative demand and depleted the soil water quickly relative to the other species. The interrelation among the morpho-anatomical leaf traits was equally affected by drought across all the studied species, suggesting that there is no clear evidence to differentiate the taxa based on drought resistance vs. drought tolerance. Thus, we have identified some easily measurable traits (i.e., LMF, RMF, and PB) which evidenced the seedling’s ability to cope with drought and which therefore could be used as proxies in the selection of drought tolerant species for reforestation in the temperate forest.