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Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish

In vertebrates, the core mechanisms that control gametogenesis are largely multiple, complex, successive, and orchestrated by intrinsic and extrinsic factors. However, age, health status, and hormonal activity are important factors for good fertility; other intangible intracellular molecular mechani...

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Autores principales: Mawed, Suzan Attia, Marini, Carlotta, Alagawany, Mahmoud, Farag, Mayada R., Reda, Rasha M., El-Saadony, Mohamed T., Elhady, Walaa M., Magi, Gian E., Di Cerbo, Alessandro, El-Nagar, Wafaa G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404823/
https://www.ncbi.nlm.nih.gov/pubmed/36009286
http://dx.doi.org/10.3390/antiox11081567
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author Mawed, Suzan Attia
Marini, Carlotta
Alagawany, Mahmoud
Farag, Mayada R.
Reda, Rasha M.
El-Saadony, Mohamed T.
Elhady, Walaa M.
Magi, Gian E.
Di Cerbo, Alessandro
El-Nagar, Wafaa G.
author_facet Mawed, Suzan Attia
Marini, Carlotta
Alagawany, Mahmoud
Farag, Mayada R.
Reda, Rasha M.
El-Saadony, Mohamed T.
Elhady, Walaa M.
Magi, Gian E.
Di Cerbo, Alessandro
El-Nagar, Wafaa G.
author_sort Mawed, Suzan Attia
collection PubMed
description In vertebrates, the core mechanisms that control gametogenesis are largely multiple, complex, successive, and orchestrated by intrinsic and extrinsic factors. However, age, health status, and hormonal activity are important factors for good fertility; other intangible intracellular molecular mechanisms that manage oocyte development are still unclear. The present study was designed to elucidate the ultrastructure changes in the ovary in response to its exposure to zinc oxide nanoparticles (ZnO-NPs) and to explore the role of autophagy and apoptosis during egg maturation and ovulation on the fertility of female zebrafish. In our study, ZnO-NPs could induce cytotoxicity in the maturing oocyte by activating autophagy and apoptosis in a caspase-dependent manner and could induce oxidative stress by generating reactive oxygen species (ROS) that elevated the mutated ovarian tP53 protein. Simultaneously, necroptosis developed, mimicking the features of apoptosis and necrosis. Collectively, ZnO-NPs created a suitable necrotic environment that led to follicular developmental retardation that altered oocyte ovulation and reduced fecundity of female zebrafish.
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spelling pubmed-94048232022-08-26 Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish Mawed, Suzan Attia Marini, Carlotta Alagawany, Mahmoud Farag, Mayada R. Reda, Rasha M. El-Saadony, Mohamed T. Elhady, Walaa M. Magi, Gian E. Di Cerbo, Alessandro El-Nagar, Wafaa G. Antioxidants (Basel) Article In vertebrates, the core mechanisms that control gametogenesis are largely multiple, complex, successive, and orchestrated by intrinsic and extrinsic factors. However, age, health status, and hormonal activity are important factors for good fertility; other intangible intracellular molecular mechanisms that manage oocyte development are still unclear. The present study was designed to elucidate the ultrastructure changes in the ovary in response to its exposure to zinc oxide nanoparticles (ZnO-NPs) and to explore the role of autophagy and apoptosis during egg maturation and ovulation on the fertility of female zebrafish. In our study, ZnO-NPs could induce cytotoxicity in the maturing oocyte by activating autophagy and apoptosis in a caspase-dependent manner and could induce oxidative stress by generating reactive oxygen species (ROS) that elevated the mutated ovarian tP53 protein. Simultaneously, necroptosis developed, mimicking the features of apoptosis and necrosis. Collectively, ZnO-NPs created a suitable necrotic environment that led to follicular developmental retardation that altered oocyte ovulation and reduced fecundity of female zebrafish. MDPI 2022-08-13 /pmc/articles/PMC9404823/ /pubmed/36009286 http://dx.doi.org/10.3390/antiox11081567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mawed, Suzan Attia
Marini, Carlotta
Alagawany, Mahmoud
Farag, Mayada R.
Reda, Rasha M.
El-Saadony, Mohamed T.
Elhady, Walaa M.
Magi, Gian E.
Di Cerbo, Alessandro
El-Nagar, Wafaa G.
Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish
title Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish
title_full Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish
title_fullStr Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish
title_full_unstemmed Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish
title_short Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish
title_sort zinc oxide nanoparticles (zno-nps) suppress fertility by activating autophagy, apoptosis, and oxidative stress in the developing oocytes of female zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404823/
https://www.ncbi.nlm.nih.gov/pubmed/36009286
http://dx.doi.org/10.3390/antiox11081567
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