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Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 ph...

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Autores principales: Kim, Shukho, Song, Hyesoon, Jin, Jong Sook, Lee, Weon Ju, Kim, Jungmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404880/
https://www.ncbi.nlm.nih.gov/pubmed/36009910
http://dx.doi.org/10.3390/antibiotics11081041
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author Kim, Shukho
Song, Hyesoon
Jin, Jong Sook
Lee, Weon Ju
Kim, Jungmin
author_facet Kim, Shukho
Song, Hyesoon
Jin, Jong Sook
Lee, Weon Ju
Kim, Jungmin
author_sort Kim, Shukho
collection PubMed
description Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT(2) buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.
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spelling pubmed-94048802022-08-26 Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients Kim, Shukho Song, Hyesoon Jin, Jong Sook Lee, Weon Ju Kim, Jungmin Antibiotics (Basel) Article Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT(2) buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages. MDPI 2022-08-02 /pmc/articles/PMC9404880/ /pubmed/36009910 http://dx.doi.org/10.3390/antibiotics11081041 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Shukho
Song, Hyesoon
Jin, Jong Sook
Lee, Weon Ju
Kim, Jungmin
Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients
title Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients
title_full Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients
title_fullStr Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients
title_full_unstemmed Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients
title_short Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients
title_sort genomic and phenotypic characterization of cutibacterium acnes bacteriophages isolated from acne patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404880/
https://www.ncbi.nlm.nih.gov/pubmed/36009910
http://dx.doi.org/10.3390/antibiotics11081041
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