Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female Dromedaries

SIMPLE SUMMARY: Heat stress imposes a high burden on domestic animals’ productive and reproductive performance. Due to the long hot summer, drought, and shortage of green fodders, camels raised in the desert suffer a lot of reproductive inefficiencies. This animal represents one of the main wealth sources for the desert inhabitants. Several fertility disorders have been discovered, leading to frequent breeding without pregnancy. This study aimed at exploring blood metabolites such as metabolic and reproductive hormones, cytokines, and antioxidants to be monitored as bio-indictors for subfertility in female camels. The results confirmed that none of the tested metabolic hormones and glucose revealed differences among fertile and subfertile females. However, FSH, inhibin, IL-ß, nitrous oxide, and glutathione revealed remarkable differences between fertile and subfertile females, which would be reliable tools to predict subfertility statuses in this animal. IL-ß revealed higher levels in the cases with genital inflammations. The normal profiles in control females revealed the highest FSH, and the lowest inhibin were vice versa in all subfertile females. Nonetheless, nitrous oxide and glutathione would also be reliable bio-indicators for judging the fertility status. ABSTRACT: The prevailing hot climate imposes heavy burdens on the productivity of the camel, goat, and sheep herds raised in the Gulf desert. Due to the lack of a reliable indicator for the various subfertility statuses in camel females, this study aimed to investigate the expression of inhibin, TGFά, ILß, FSH, sex and metabolic hormones, and antioxidants for the fertility status in camel females. Eighty-two subfertile and five fertile females were admitted to the university clinic with the complaint of repeat breeding with failed conception. The animal’s genital tracts were examined for reproductive soundness. Blood samples were withdrawn for hormonal, cytokines, and antioxidants determinations. Subfertile females were categorized into six groups; endometritis (EN, 28), inactive ovaries (IO, 20), ovarian hydrobursitis (BU, 19), vaginal adhesions (VA, 7), salpingitis (SA, 4), and cervicitis (CE, 4). Results revealed a significant increase in inhibin in all groups compared to control (68.2, 66.4, 61.8. 58.8, 58.3, 55.8, and 36 pg/mL, in CE, VA, IO, BU, EN, SA, and CON, respectively). TGFά, dehydroepiandrosterone (DHEA), and progesterone were not different among groups, whereas IL-ß differed among groups. FSH, estradiol, nitrous oxide, and glutathione were higher in CON compared with other groups. In conclusion, reproductive failures in camel females are reflected in the imbalances of endocrine, cytokines, and antioxidants bio-indicators.