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Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China

The emergence of the mobile tigecycline-resistance gene, tet(X4), poses a significant threat to public health. To investigate the prevalence and genetic characteristics of the tet(X4)-positive Escherichia coli in humans, 1101 human stool samples were collected from a tertiary class-A hospital in Bei...

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Autores principales: Zhai, Weishuai, Tian, Yingxin, Shao, Dongyan, Zhang, Muchen, Li, Jiyun, Song, Huangwei, Sun, Chengtao, Wang, Yang, Liu, Dejun, Zhang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405050/
https://www.ncbi.nlm.nih.gov/pubmed/36009937
http://dx.doi.org/10.3390/antibiotics11081068
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author Zhai, Weishuai
Tian, Yingxin
Shao, Dongyan
Zhang, Muchen
Li, Jiyun
Song, Huangwei
Sun, Chengtao
Wang, Yang
Liu, Dejun
Zhang, Ying
author_facet Zhai, Weishuai
Tian, Yingxin
Shao, Dongyan
Zhang, Muchen
Li, Jiyun
Song, Huangwei
Sun, Chengtao
Wang, Yang
Liu, Dejun
Zhang, Ying
author_sort Zhai, Weishuai
collection PubMed
description The emergence of the mobile tigecycline-resistance gene, tet(X4), poses a significant threat to public health. To investigate the prevalence and genetic characteristics of the tet(X4)-positive Escherichia coli in humans, 1101 human stool samples were collected from a tertiary class-A hospital in Beijing, China, in 2019. Eight E. coli isolates that were positive for tet(X4) were identified from clinical departments of oncology (n = 3), hepatology (n = 2), nephrology (n = 1), urology (n = 1), and general surgery (n = 1). They exhibited resistance to multiple antibiotics, including tigecycline, but remained susceptible to meropenem and polymyxin B. A phylogenetic analysis revealed that the clonal spread of four tet(X4)-positive E. coli from different periods of time or departments existed in this hospital, and three isolates were phylogenetically close to the tet(X4)-positive E. coli from animals and the environment. All tet(X4)-positive E. coli isolates contained the IncX1-plasmid replicon. Three isolates successfully transferred their tigecycline resistance to the recipient strain, C600, demonstrating that the plasmid-mediated horizontal gene transfer constitutes another critical mechanism for transmitting tet(X4). Notably, all tet(X4)-bearing plasmids identified in this study had a high similarity to several plasmids recovered from animal-derived strains. Our findings revealed the importance of both the clonal spread and horizontal gene transfer in the spread of tet(X4) within human clinics and between different sources.
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spelling pubmed-94050502022-08-26 Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China Zhai, Weishuai Tian, Yingxin Shao, Dongyan Zhang, Muchen Li, Jiyun Song, Huangwei Sun, Chengtao Wang, Yang Liu, Dejun Zhang, Ying Antibiotics (Basel) Article The emergence of the mobile tigecycline-resistance gene, tet(X4), poses a significant threat to public health. To investigate the prevalence and genetic characteristics of the tet(X4)-positive Escherichia coli in humans, 1101 human stool samples were collected from a tertiary class-A hospital in Beijing, China, in 2019. Eight E. coli isolates that were positive for tet(X4) were identified from clinical departments of oncology (n = 3), hepatology (n = 2), nephrology (n = 1), urology (n = 1), and general surgery (n = 1). They exhibited resistance to multiple antibiotics, including tigecycline, but remained susceptible to meropenem and polymyxin B. A phylogenetic analysis revealed that the clonal spread of four tet(X4)-positive E. coli from different periods of time or departments existed in this hospital, and three isolates were phylogenetically close to the tet(X4)-positive E. coli from animals and the environment. All tet(X4)-positive E. coli isolates contained the IncX1-plasmid replicon. Three isolates successfully transferred their tigecycline resistance to the recipient strain, C600, demonstrating that the plasmid-mediated horizontal gene transfer constitutes another critical mechanism for transmitting tet(X4). Notably, all tet(X4)-bearing plasmids identified in this study had a high similarity to several plasmids recovered from animal-derived strains. Our findings revealed the importance of both the clonal spread and horizontal gene transfer in the spread of tet(X4) within human clinics and between different sources. MDPI 2022-08-06 /pmc/articles/PMC9405050/ /pubmed/36009937 http://dx.doi.org/10.3390/antibiotics11081068 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhai, Weishuai
Tian, Yingxin
Shao, Dongyan
Zhang, Muchen
Li, Jiyun
Song, Huangwei
Sun, Chengtao
Wang, Yang
Liu, Dejun
Zhang, Ying
Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China
title Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China
title_full Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China
title_fullStr Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China
title_full_unstemmed Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China
title_short Fecal Carriage of Escherichia coli Harboring the tet(X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China
title_sort fecal carriage of escherichia coli harboring the tet(x4)-incx1 plasmid from a tertiary class-a hospital in beijing, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405050/
https://www.ncbi.nlm.nih.gov/pubmed/36009937
http://dx.doi.org/10.3390/antibiotics11081068
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