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Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus

SIMPLE SUMMARY: Nesfatin-1 (Nesf-1) is a neuropeptide that plays important roles in regulating food intake, mainly related to its anorexigenic effect, and it is mainly distributed in the digestive systems of all vertebrates. With this study, we expand knowledge on the localization of Nesf-1 in the d...

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Autores principales: De Felice, Elena, Gatta, Claudia, Giaquinto, Daniela, Fioretto, Federica, Maruccio, Lucianna, d’Angelo, Danila, Scocco, Paola, de Girolamo, Paolo, D’Angelo, Livia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405072/
https://www.ncbi.nlm.nih.gov/pubmed/36009738
http://dx.doi.org/10.3390/ani12162148
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author De Felice, Elena
Gatta, Claudia
Giaquinto, Daniela
Fioretto, Federica
Maruccio, Lucianna
d’Angelo, Danila
Scocco, Paola
de Girolamo, Paolo
D’Angelo, Livia
author_facet De Felice, Elena
Gatta, Claudia
Giaquinto, Daniela
Fioretto, Federica
Maruccio, Lucianna
d’Angelo, Danila
Scocco, Paola
de Girolamo, Paolo
D’Angelo, Livia
author_sort De Felice, Elena
collection PubMed
description SIMPLE SUMMARY: Nesfatin-1 (Nesf-1) is a neuropeptide that plays important roles in regulating food intake, mainly related to its anorexigenic effect, and it is mainly distributed in the digestive systems of all vertebrates. With this study, we expand knowledge on the localization of Nesf-1 in the digestive tract of an aquatic mammalian species, the common bottlenose dolphin (Tursiops truncatus), allowing comparative study on terrestrial mammals. Dolphin tissue samples (three gastric chambers and intestine) were provided by the Mediterranean Marine Mammal Tissue Bank of the Department of Comparative Biomedicine and Food Science of the University of Padova (Italy). ABSTRACT: First identified as an anorexigenic peptide, in the last decades, several studies have suggested that Nesfatin-1 (Nesf-1) is a pleiotropic hormone implicated in numerous regulatory processes in peripheral organs and tissues. In vertebrates, Nesf-1 is indeed expressed in the central nervous system and peripheral organs. In this study, we characterized the pattern of Nesf-1 distribution within the digestive tract of the common bottlenose dolphin (Tursiops truncatus), composed of three gastric chambers and an intestine without a clear subdivision in the small and large intestine, also lacking a caecum. Our results indicated that Nesf-1 is widely distributed in cells of the mucosal epithelium of the gastric chambers. Most of the immunoreactivity was observed in the second chamber, compared to the first and third chambers. Immunopositivity was also found in nerve fibers and neurons, scattered or/and clustered in ganglion structures along all the examined gastrointestinal tracts. These observations add new data on the highly conserved role of Nesf-1 in the mammalian digestive system.
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spelling pubmed-94050722022-08-26 Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus De Felice, Elena Gatta, Claudia Giaquinto, Daniela Fioretto, Federica Maruccio, Lucianna d’Angelo, Danila Scocco, Paola de Girolamo, Paolo D’Angelo, Livia Animals (Basel) Communication SIMPLE SUMMARY: Nesfatin-1 (Nesf-1) is a neuropeptide that plays important roles in regulating food intake, mainly related to its anorexigenic effect, and it is mainly distributed in the digestive systems of all vertebrates. With this study, we expand knowledge on the localization of Nesf-1 in the digestive tract of an aquatic mammalian species, the common bottlenose dolphin (Tursiops truncatus), allowing comparative study on terrestrial mammals. Dolphin tissue samples (three gastric chambers and intestine) were provided by the Mediterranean Marine Mammal Tissue Bank of the Department of Comparative Biomedicine and Food Science of the University of Padova (Italy). ABSTRACT: First identified as an anorexigenic peptide, in the last decades, several studies have suggested that Nesfatin-1 (Nesf-1) is a pleiotropic hormone implicated in numerous regulatory processes in peripheral organs and tissues. In vertebrates, Nesf-1 is indeed expressed in the central nervous system and peripheral organs. In this study, we characterized the pattern of Nesf-1 distribution within the digestive tract of the common bottlenose dolphin (Tursiops truncatus), composed of three gastric chambers and an intestine without a clear subdivision in the small and large intestine, also lacking a caecum. Our results indicated that Nesf-1 is widely distributed in cells of the mucosal epithelium of the gastric chambers. Most of the immunoreactivity was observed in the second chamber, compared to the first and third chambers. Immunopositivity was also found in nerve fibers and neurons, scattered or/and clustered in ganglion structures along all the examined gastrointestinal tracts. These observations add new data on the highly conserved role of Nesf-1 in the mammalian digestive system. MDPI 2022-08-22 /pmc/articles/PMC9405072/ /pubmed/36009738 http://dx.doi.org/10.3390/ani12162148 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
De Felice, Elena
Gatta, Claudia
Giaquinto, Daniela
Fioretto, Federica
Maruccio, Lucianna
d’Angelo, Danila
Scocco, Paola
de Girolamo, Paolo
D’Angelo, Livia
Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus
title Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus
title_full Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus
title_fullStr Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus
title_full_unstemmed Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus
title_short Immunolocalization of Nesfatin-1 in the Gastrointestinal Tract of the Common Bottlenose Dolphin Tursiops truncatus
title_sort immunolocalization of nesfatin-1 in the gastrointestinal tract of the common bottlenose dolphin tursiops truncatus
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405072/
https://www.ncbi.nlm.nih.gov/pubmed/36009738
http://dx.doi.org/10.3390/ani12162148
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