SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos

Traditional fluorescent proteins exhibit limitations in brightness and photostability that hinder optimal characterization of the dynamic cellular behavior of proteins of interest. SNAP- and Halo-tagging are alternatives to traditional fluorescent protein tagging utilizing bright, stable chemical dy...

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Detalles Bibliográficos
Autores principales: Dudley, Claire E., van den Goor, Lotte, Miller, Ann L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405085/
https://www.ncbi.nlm.nih.gov/pubmed/36035797
http://dx.doi.org/10.1016/j.xpro.2022.101622

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405085/
https://www.ncbi.nlm.nih.gov/pubmed/36035797
http://dx.doi.org/10.1016/j.xpro.2022.101622

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