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SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos
Traditional fluorescent proteins exhibit limitations in brightness and photostability that hinder optimal characterization of the dynamic cellular behavior of proteins of interest. SNAP- and Halo-tagging are alternatives to traditional fluorescent protein tagging utilizing bright, stable chemical dy...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405085/ https://www.ncbi.nlm.nih.gov/pubmed/36035797 http://dx.doi.org/10.1016/j.xpro.2022.101622 |