Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue

An imaging technique across multiple spatial scales is required for extracting structural information on neurons with processes of meter scale length and specialized nanoscale structures. Here, we present a protocol combining multi-scale light microscopy (LM) with electron microscopy (EM) in mouse b...

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Detalles Bibliográficos
Autores principales: Yamauchi, Kenta, Furuta, Takahiro, Okamoto, Shinichiro, Takahashi, Megumu, Koike, Masato, Hioki, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405099/
https://www.ncbi.nlm.nih.gov/pubmed/36035789
http://dx.doi.org/10.1016/j.xpro.2022.101508
Descripción
Sumario:An imaging technique across multiple spatial scales is required for extracting structural information on neurons with processes of meter scale length and specialized nanoscale structures. Here, we present a protocol combining multi-scale light microscopy (LM) with electron microscopy (EM) in mouse brain tissue. We describe tissue slice preparation and LM/EM dual labeling with EGFP-APEX2 fusion protein. We then detail ScaleSF tissue clearing and successive LM/EM imaging. Our protocol allows for deciphering structural information across multiple spatial scales on neurons. For complete details on the use and execution of this protocol, please refer to Furuta et al. (2022).

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