A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and uncontrolled systems to simplified and relatively robust protocols, using the knowledge and cues gathered at each step. H...

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Detalles Bibliográficos
Autores principales: Lyra-Leite, Davi M., Gutiérrez-Gutiérrez, Óscar, Wang, Meimei, Zhou, Yang, Cyganek, Lukas, Burridge, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405110/
https://www.ncbi.nlm.nih.gov/pubmed/36035804
http://dx.doi.org/10.1016/j.xpro.2022.101560
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author Lyra-Leite, Davi M.
Gutiérrez-Gutiérrez, Óscar
Wang, Meimei
Zhou, Yang
Cyganek, Lukas
Burridge, Paul W.
author_facet Lyra-Leite, Davi M.
Gutiérrez-Gutiérrez, Óscar
Wang, Meimei
Zhou, Yang
Cyganek, Lukas
Burridge, Paul W.
author_sort Lyra-Leite, Davi M.
collection PubMed
description The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and uncontrolled systems to simplified and relatively robust protocols, using the knowledge and cues gathered at each step. HiPSC-derived cardiomyocytes have proven to be a useful tool in human disease modelling, drug discovery, developmental biology, and regenerative medicine. In this protocol review, we will highlight the evolution of protocols associated with hPSC culture, cardiomyocyte differentiation, sub-type specification, and cardiomyocyte maturation. We also discuss protocols for somatic cell direct reprogramming to cardiomyocyte-like cells.
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spelling pubmed-94051102022-08-26 A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming Lyra-Leite, Davi M. Gutiérrez-Gutiérrez, Óscar Wang, Meimei Zhou, Yang Cyganek, Lukas Burridge, Paul W. STAR Protoc Protocol Review The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and uncontrolled systems to simplified and relatively robust protocols, using the knowledge and cues gathered at each step. HiPSC-derived cardiomyocytes have proven to be a useful tool in human disease modelling, drug discovery, developmental biology, and regenerative medicine. In this protocol review, we will highlight the evolution of protocols associated with hPSC culture, cardiomyocyte differentiation, sub-type specification, and cardiomyocyte maturation. We also discuss protocols for somatic cell direct reprogramming to cardiomyocyte-like cells. Elsevier 2022-08-18 /pmc/articles/PMC9405110/ /pubmed/36035804 http://dx.doi.org/10.1016/j.xpro.2022.101560 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol Review
Lyra-Leite, Davi M.
Gutiérrez-Gutiérrez, Óscar
Wang, Meimei
Zhou, Yang
Cyganek, Lukas
Burridge, Paul W.
A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
title A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
title_full A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
title_fullStr A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
title_full_unstemmed A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
title_short A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
title_sort review of protocols for human ipsc culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
topic Protocol Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405110/
https://www.ncbi.nlm.nih.gov/pubmed/36035804
http://dx.doi.org/10.1016/j.xpro.2022.101560
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