A Survey of Genome-Wide Genetic Characterizations of Crossbred Dairy Cattle in Local Farms in Cambodia

SIMPLE SUMMARY: The Cambodian dairy sector collapsed due to the civil war. Thereby, the Cambodia dairy sector has not obtained basic information, such as cattle breed and milk production. To encourage the recovery of the Cambodian dairy sector, the present study aimed to reveal the genetic variation and the milk production in Cambodian crossbred dairy cattle. Initially, we conducted interviews to understand the breeding background and milk production of two local farms (Farm R and Farm M) in Cambodia. The percentage (%) of milk fat content in Farm R was higher than that in Farm M. A genome-wide analysis of the genetic characterization for 75 cows in the two dairy farms implies that some cows in Farm R are genetically far from other crossbred cattle and retain a higher proportion of genetic background derived from Cambodian-native cattle. The present study indicated genetic characteristics and milk composition in Cambodian crossbred cattle. Gene character in Cambodian local crossbred cattle could contribute to milk production in the Cambodian dairy system. Thus, our basic genetic study could provide a new breeding strategy by using local Cambodian crossbred dairy cattle to establish an adequate dairy strain. ABSTRACT: To improve the dairy sector in Cambodia in the future, we aimed to reveal the genetic variation and the milk production in Cambodian crossbred dairy cattle. We calculated the percent (%) milk fat content and the average milk yield per cow (L/day) for two farms (Farm R and M) based on the farmers’ records and interviews. The crossbred cows originated from Cambodian local farmers and Thailand breeders in Farm R, whereas the crossbred cows originated in Thailand breeders in Farm M. Then, we performed genetic characterization for 75 individuals from the two farms and an individual Japanese pure Holstein-Friesian cow based on 133,705 single nucleotide polymorphisms (SNPs) obtained by the GRAS-Di method. The milk fat contents in the bulk milk in the dry season and the average milk yield per cow on Farm R were 3.77 ± 0.98% and 7.81 ± 2.66 L/day, respectively, and were higher than those on Farm M (3.35 ± 0.54% and 6.5–7.5 L/day). Cattle originating in Cambodia in Farm R possessed a unique genetic character different from cattle from Thailand in Farm M. The present study suggests that the differences in milk fat content between the two farms might be explained by the genetic differences in crossbred cows.