Cargando…

Tuning the Biological Activity of Camphorimine Complexes through Metal Selection

The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the...

Descripción completa

Detalles Bibliográficos
Autores principales: Costa, Joana P., Pinheiro, Teresa, Martins, Maria S., Carvalho, M. Fernanda N. N., Feliciano, Joana R., Leitão, Jorge H., Silva, Rafaela A. L., Guerreiro, Joana F., Alves, Luís M. C., Custódio, Inês, Cruz, João, Marques, Fernanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405135/
https://www.ncbi.nlm.nih.gov/pubmed/36009879
http://dx.doi.org/10.3390/antibiotics11081010
_version_ 1784773806478327808
author Costa, Joana P.
Pinheiro, Teresa
Martins, Maria S.
Carvalho, M. Fernanda N. N.
Feliciano, Joana R.
Leitão, Jorge H.
Silva, Rafaela A. L.
Guerreiro, Joana F.
Alves, Luís M. C.
Custódio, Inês
Cruz, João
Marques, Fernanda
author_facet Costa, Joana P.
Pinheiro, Teresa
Martins, Maria S.
Carvalho, M. Fernanda N. N.
Feliciano, Joana R.
Leitão, Jorge H.
Silva, Rafaela A. L.
Guerreiro, Joana F.
Alves, Luís M. C.
Custódio, Inês
Cruz, João
Marques, Fernanda
author_sort Costa, Joana P.
collection PubMed
description The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the silver complexes, which in turn were ca. one order of magnitude more active than the copper complexes. An important finding was that the cytotoxic activity of the Ag(I) and Au(I) camphorimine complexes was higher than that of cisplatin. Another relevant aspect was that the camphorimine complexes did not interact significantly with DNA, in contrast with cisplatin. The cytotoxic activity of the camphorimine complexes displayed a direct relationship with the cellular uptake by OVCAR3 cells, as ascertained by PIXE (particle-induced X-ray emission). The levels of ROS (reactive oxygen species) formation exhibited an inverse relationship with the reduction potentials for the complexes with the same metal, as assessed by cyclic voltammetry. In order to gain insight into the toxicity of the complexes, their cytotoxicity toward nontumoral cells (HDF and V79 fibroblasts) was evaluated. The in vivo cytotoxicity of complex 5 using the nematode Caenorhabditis elegans was also assessed. The silver camphorimine complexes displayed the highest selectivity coefficients (activity vs. toxicity).
format Online
Article
Text
id pubmed-9405135
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94051352022-08-26 Tuning the Biological Activity of Camphorimine Complexes through Metal Selection Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana R. Leitão, Jorge H. Silva, Rafaela A. L. Guerreiro, Joana F. Alves, Luís M. C. Custódio, Inês Cruz, João Marques, Fernanda Antibiotics (Basel) Article The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the silver complexes, which in turn were ca. one order of magnitude more active than the copper complexes. An important finding was that the cytotoxic activity of the Ag(I) and Au(I) camphorimine complexes was higher than that of cisplatin. Another relevant aspect was that the camphorimine complexes did not interact significantly with DNA, in contrast with cisplatin. The cytotoxic activity of the camphorimine complexes displayed a direct relationship with the cellular uptake by OVCAR3 cells, as ascertained by PIXE (particle-induced X-ray emission). The levels of ROS (reactive oxygen species) formation exhibited an inverse relationship with the reduction potentials for the complexes with the same metal, as assessed by cyclic voltammetry. In order to gain insight into the toxicity of the complexes, their cytotoxicity toward nontumoral cells (HDF and V79 fibroblasts) was evaluated. The in vivo cytotoxicity of complex 5 using the nematode Caenorhabditis elegans was also assessed. The silver camphorimine complexes displayed the highest selectivity coefficients (activity vs. toxicity). MDPI 2022-07-27 /pmc/articles/PMC9405135/ /pubmed/36009879 http://dx.doi.org/10.3390/antibiotics11081010 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Costa, Joana P.
Pinheiro, Teresa
Martins, Maria S.
Carvalho, M. Fernanda N. N.
Feliciano, Joana R.
Leitão, Jorge H.
Silva, Rafaela A. L.
Guerreiro, Joana F.
Alves, Luís M. C.
Custódio, Inês
Cruz, João
Marques, Fernanda
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
title Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
title_full Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
title_fullStr Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
title_full_unstemmed Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
title_short Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
title_sort tuning the biological activity of camphorimine complexes through metal selection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405135/
https://www.ncbi.nlm.nih.gov/pubmed/36009879
http://dx.doi.org/10.3390/antibiotics11081010
work_keys_str_mv AT costajoanap tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT pinheiroteresa tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT martinsmarias tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT carvalhomfernandann tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT felicianojoanar tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT leitaojorgeh tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT silvarafaelaal tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT guerreirojoanaf tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT alvesluismc tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT custodioines tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT cruzjoao tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection
AT marquesfernanda tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection