Cargando…
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405135/ https://www.ncbi.nlm.nih.gov/pubmed/36009879 http://dx.doi.org/10.3390/antibiotics11081010 |
_version_ | 1784773806478327808 |
---|---|
author | Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana R. Leitão, Jorge H. Silva, Rafaela A. L. Guerreiro, Joana F. Alves, Luís M. C. Custódio, Inês Cruz, João Marques, Fernanda |
author_facet | Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana R. Leitão, Jorge H. Silva, Rafaela A. L. Guerreiro, Joana F. Alves, Luís M. C. Custódio, Inês Cruz, João Marques, Fernanda |
author_sort | Costa, Joana P. |
collection | PubMed |
description | The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the silver complexes, which in turn were ca. one order of magnitude more active than the copper complexes. An important finding was that the cytotoxic activity of the Ag(I) and Au(I) camphorimine complexes was higher than that of cisplatin. Another relevant aspect was that the camphorimine complexes did not interact significantly with DNA, in contrast with cisplatin. The cytotoxic activity of the camphorimine complexes displayed a direct relationship with the cellular uptake by OVCAR3 cells, as ascertained by PIXE (particle-induced X-ray emission). The levels of ROS (reactive oxygen species) formation exhibited an inverse relationship with the reduction potentials for the complexes with the same metal, as assessed by cyclic voltammetry. In order to gain insight into the toxicity of the complexes, their cytotoxicity toward nontumoral cells (HDF and V79 fibroblasts) was evaluated. The in vivo cytotoxicity of complex 5 using the nematode Caenorhabditis elegans was also assessed. The silver camphorimine complexes displayed the highest selectivity coefficients (activity vs. toxicity). |
format | Online Article Text |
id | pubmed-9405135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94051352022-08-26 Tuning the Biological Activity of Camphorimine Complexes through Metal Selection Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana R. Leitão, Jorge H. Silva, Rafaela A. L. Guerreiro, Joana F. Alves, Luís M. C. Custódio, Inês Cruz, João Marques, Fernanda Antibiotics (Basel) Article The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the silver complexes, which in turn were ca. one order of magnitude more active than the copper complexes. An important finding was that the cytotoxic activity of the Ag(I) and Au(I) camphorimine complexes was higher than that of cisplatin. Another relevant aspect was that the camphorimine complexes did not interact significantly with DNA, in contrast with cisplatin. The cytotoxic activity of the camphorimine complexes displayed a direct relationship with the cellular uptake by OVCAR3 cells, as ascertained by PIXE (particle-induced X-ray emission). The levels of ROS (reactive oxygen species) formation exhibited an inverse relationship with the reduction potentials for the complexes with the same metal, as assessed by cyclic voltammetry. In order to gain insight into the toxicity of the complexes, their cytotoxicity toward nontumoral cells (HDF and V79 fibroblasts) was evaluated. The in vivo cytotoxicity of complex 5 using the nematode Caenorhabditis elegans was also assessed. The silver camphorimine complexes displayed the highest selectivity coefficients (activity vs. toxicity). MDPI 2022-07-27 /pmc/articles/PMC9405135/ /pubmed/36009879 http://dx.doi.org/10.3390/antibiotics11081010 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana R. Leitão, Jorge H. Silva, Rafaela A. L. Guerreiro, Joana F. Alves, Luís M. C. Custódio, Inês Cruz, João Marques, Fernanda Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
title | Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
title_full | Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
title_fullStr | Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
title_full_unstemmed | Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
title_short | Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
title_sort | tuning the biological activity of camphorimine complexes through metal selection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405135/ https://www.ncbi.nlm.nih.gov/pubmed/36009879 http://dx.doi.org/10.3390/antibiotics11081010 |
work_keys_str_mv | AT costajoanap tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT pinheiroteresa tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT martinsmarias tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT carvalhomfernandann tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT felicianojoanar tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT leitaojorgeh tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT silvarafaelaal tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT guerreirojoanaf tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT alvesluismc tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT custodioines tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT cruzjoao tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection AT marquesfernanda tuningthebiologicalactivityofcamphoriminecomplexesthroughmetalselection |