Cargando…

Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides

Bacteria can evade antibiotics by acquiring resistance genes, as well as switching to a non-growing dormant state without accompanying genetic modification. Bacteria in this quiescent state are called persisters, and this non-inheritable ability to withstand multiple antibiotics is referred to as an...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Minjeong, Choi, Tae-Rim, Ahn, Soyeon, Heo, Hee Young, Kim, Hyerim, Lee, Hye Soo, Lee, Yoo Kyung, Joo, Hwang-Soo, Yune, Philip S., Kim, Wooseong, Yang, Yung-Hun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405152/
https://www.ncbi.nlm.nih.gov/pubmed/36009888
http://dx.doi.org/10.3390/antibiotics11081019
_version_ 1784773810613911552
author Kang, Minjeong
Choi, Tae-Rim
Ahn, Soyeon
Heo, Hee Young
Kim, Hyerim
Lee, Hye Soo
Lee, Yoo Kyung
Joo, Hwang-Soo
Yune, Philip S.
Kim, Wooseong
Yang, Yung-Hun
author_facet Kang, Minjeong
Choi, Tae-Rim
Ahn, Soyeon
Heo, Hee Young
Kim, Hyerim
Lee, Hye Soo
Lee, Yoo Kyung
Joo, Hwang-Soo
Yune, Philip S.
Kim, Wooseong
Yang, Yung-Hun
author_sort Kang, Minjeong
collection PubMed
description Bacteria can evade antibiotics by acquiring resistance genes, as well as switching to a non-growing dormant state without accompanying genetic modification. Bacteria in this quiescent state are called persisters, and this non-inheritable ability to withstand multiple antibiotics is referred to as antibiotic tolerance. Although all bacteria are considered to be able to form antibiotic-tolerant persisters, the antibiotic tolerance of extremophilic bacteria is poorly understood. Previously, we identified the psychrotolerant bacterium Pseudomonas sp. B14-6 from the glacier foreland of Midtre Lovénbreen in High Arctic Svalbard. Herein, we investigated the resistance and tolerance of Pseudomonas sp. B14-6 against aminoglycosides at various temperatures. This bacterium was resistant to streptomycin and susceptible to apramycin, gentamicin, kanamycin, and tobramycin. The two putative aminoglycoside phosphotransferase genes aph1 and aph2 were the most likely contributors to streptomycin resistance. Notably, unlike the mesophilic Pseudomonas aeruginosa PA14, this cold-adapted bacterium demonstrated reduced susceptibility to all tested aminoglycosides in a temperature-dependent manner. Pseudomonas sp. B14-6 at a lower temperature formed the persister cells that shows tolerance to the 100-fold minimum inhibitory concentration (MIC) of gentamicin, as well as the partially tolerant cells that withstand 25-fold MIC gentamicin. The temperature-dependent gentamicin tolerance appears to result from reduced metabolic activity. Lastly, the partially tolerant Pseudomonas sp. B14-6 cells could slowly proliferate under the bactericidal concentrations of aminoglycosides. Our results demonstrate that Pseudomonas sp. B14-6 has a characteristic ability to form cells with a range of tolerance, which appears to be inversely proportional to its growth rate.
format Online
Article
Text
id pubmed-9405152
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94051522022-08-26 Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides Kang, Minjeong Choi, Tae-Rim Ahn, Soyeon Heo, Hee Young Kim, Hyerim Lee, Hye Soo Lee, Yoo Kyung Joo, Hwang-Soo Yune, Philip S. Kim, Wooseong Yang, Yung-Hun Antibiotics (Basel) Article Bacteria can evade antibiotics by acquiring resistance genes, as well as switching to a non-growing dormant state without accompanying genetic modification. Bacteria in this quiescent state are called persisters, and this non-inheritable ability to withstand multiple antibiotics is referred to as antibiotic tolerance. Although all bacteria are considered to be able to form antibiotic-tolerant persisters, the antibiotic tolerance of extremophilic bacteria is poorly understood. Previously, we identified the psychrotolerant bacterium Pseudomonas sp. B14-6 from the glacier foreland of Midtre Lovénbreen in High Arctic Svalbard. Herein, we investigated the resistance and tolerance of Pseudomonas sp. B14-6 against aminoglycosides at various temperatures. This bacterium was resistant to streptomycin and susceptible to apramycin, gentamicin, kanamycin, and tobramycin. The two putative aminoglycoside phosphotransferase genes aph1 and aph2 were the most likely contributors to streptomycin resistance. Notably, unlike the mesophilic Pseudomonas aeruginosa PA14, this cold-adapted bacterium demonstrated reduced susceptibility to all tested aminoglycosides in a temperature-dependent manner. Pseudomonas sp. B14-6 at a lower temperature formed the persister cells that shows tolerance to the 100-fold minimum inhibitory concentration (MIC) of gentamicin, as well as the partially tolerant cells that withstand 25-fold MIC gentamicin. The temperature-dependent gentamicin tolerance appears to result from reduced metabolic activity. Lastly, the partially tolerant Pseudomonas sp. B14-6 cells could slowly proliferate under the bactericidal concentrations of aminoglycosides. Our results demonstrate that Pseudomonas sp. B14-6 has a characteristic ability to form cells with a range of tolerance, which appears to be inversely proportional to its growth rate. MDPI 2022-07-29 /pmc/articles/PMC9405152/ /pubmed/36009888 http://dx.doi.org/10.3390/antibiotics11081019 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Minjeong
Choi, Tae-Rim
Ahn, Soyeon
Heo, Hee Young
Kim, Hyerim
Lee, Hye Soo
Lee, Yoo Kyung
Joo, Hwang-Soo
Yune, Philip S.
Kim, Wooseong
Yang, Yung-Hun
Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides
title Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides
title_full Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides
title_fullStr Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides
title_full_unstemmed Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides
title_short Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides
title_sort arctic psychrotolerant pseudomonas sp. b14-6 exhibits temperature-dependent susceptibility to aminoglycosides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405152/
https://www.ncbi.nlm.nih.gov/pubmed/36009888
http://dx.doi.org/10.3390/antibiotics11081019
work_keys_str_mv AT kangminjeong arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT choitaerim arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT ahnsoyeon arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT heoheeyoung arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT kimhyerim arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT leehyesoo arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT leeyookyung arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT joohwangsoo arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT yunephilips arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT kimwooseong arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides
AT yangyunghun arcticpsychrotolerantpseudomonasspb146exhibitstemperaturedependentsusceptibilitytoaminoglycosides