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Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis
RNA-sequencing (RNA-seq) is a versatile, high-throughput technology that is being widely employed for screening differentially expressed genes (DEGs) in various diseases. Echinococcosis, a globally distributed zoonosis, has been reported to impose a heavy disease burden in pastoral areas of China. H...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405190/ https://www.ncbi.nlm.nih.gov/pubmed/36034715 http://dx.doi.org/10.3389/fcimb.2022.913393 |
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author | Liu, Lei Chen, Fan Jiang, Shan Zhong, Bo Li, Wei Xu, Kejun Wang, Qi Wang, Ying Cao, Jianping |
author_facet | Liu, Lei Chen, Fan Jiang, Shan Zhong, Bo Li, Wei Xu, Kejun Wang, Qi Wang, Ying Cao, Jianping |
author_sort | Liu, Lei |
collection | PubMed |
description | RNA-sequencing (RNA-seq) is a versatile, high-throughput technology that is being widely employed for screening differentially expressed genes (DEGs) in various diseases. Echinococcosis, a globally distributed zoonosis, has been reported to impose a heavy disease burden in pastoral areas of China. Herein we aimed to explore the molecular mechanisms underlying echinococcosis. In this study, peripheral blood samples were collected from six patients with alveolar echinococcosis (AE), six patients with cystic echinococcosis (CE), and six healthy controls. RNA-Seq (mRNA) was performed to detect gene transcript and expression levels, and DEGs were subjected to bioinformatic analyses. In comparison with healthy controls, 492 DEGs (270 upregulated, 222 downregulated) were found in the AE group and 424 DEGs (170 upregulated, 254 downregulated) were found in the CE group (|log(2) (fold change)| > 1 and P < 0.05). Further, 60 genes were upregulated and 39 were downregulated in both the AE and CE groups. Gene ontology enrichment analysis indicated that DEGs were mainly involved in molecular functions, including extracellular space, extracellular region, organ and system development, and anatomical structure development. Protein–protein interaction (PPI) networks were constructed to depict the complex relationship between DEGs and interacting proteins. |
format | Online Article Text |
id | pubmed-9405190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94051902022-08-26 Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis Liu, Lei Chen, Fan Jiang, Shan Zhong, Bo Li, Wei Xu, Kejun Wang, Qi Wang, Ying Cao, Jianping Front Cell Infect Microbiol Cellular and Infection Microbiology RNA-sequencing (RNA-seq) is a versatile, high-throughput technology that is being widely employed for screening differentially expressed genes (DEGs) in various diseases. Echinococcosis, a globally distributed zoonosis, has been reported to impose a heavy disease burden in pastoral areas of China. Herein we aimed to explore the molecular mechanisms underlying echinococcosis. In this study, peripheral blood samples were collected from six patients with alveolar echinococcosis (AE), six patients with cystic echinococcosis (CE), and six healthy controls. RNA-Seq (mRNA) was performed to detect gene transcript and expression levels, and DEGs were subjected to bioinformatic analyses. In comparison with healthy controls, 492 DEGs (270 upregulated, 222 downregulated) were found in the AE group and 424 DEGs (170 upregulated, 254 downregulated) were found in the CE group (|log(2) (fold change)| > 1 and P < 0.05). Further, 60 genes were upregulated and 39 were downregulated in both the AE and CE groups. Gene ontology enrichment analysis indicated that DEGs were mainly involved in molecular functions, including extracellular space, extracellular region, organ and system development, and anatomical structure development. Protein–protein interaction (PPI) networks were constructed to depict the complex relationship between DEGs and interacting proteins. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9405190/ /pubmed/36034715 http://dx.doi.org/10.3389/fcimb.2022.913393 Text en Copyright © 2022 Liu, Chen, Jiang, Zhong, Li, Xu, Wang, Wang and Cao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Liu, Lei Chen, Fan Jiang, Shan Zhong, Bo Li, Wei Xu, Kejun Wang, Qi Wang, Ying Cao, Jianping Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
title | Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
title_full | Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
title_fullStr | Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
title_full_unstemmed | Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
title_short | Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
title_sort | analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405190/ https://www.ncbi.nlm.nih.gov/pubmed/36034715 http://dx.doi.org/10.3389/fcimb.2022.913393 |
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