A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species

Using alkyl halides to tag reactive sulfur species (RSSs) (H(2)S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this supposition is facing...

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Autores principales: Wu, Xiaohua, Xin, Yuping, Wang, Qingda, Xia, Yongzhen, Xun, Luying, Liu, Huaiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405219/
https://www.ncbi.nlm.nih.gov/pubmed/36009302
http://dx.doi.org/10.3390/antiox11081583
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author Wu, Xiaohua
Xin, Yuping
Wang, Qingda
Xia, Yongzhen
Xun, Luying
Liu, Huaiwei
author_facet Wu, Xiaohua
Xin, Yuping
Wang, Qingda
Xia, Yongzhen
Xun, Luying
Liu, Huaiwei
author_sort Wu, Xiaohua
collection PubMed
description Using alkyl halides to tag reactive sulfur species (RSSs) (H(2)S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this supposition is facing a challenge. RSS also initiates a reductive dehalogenation reaction, which generates the reduced unloaded tag and oxidized RSS. Therefore, RSS content in bio-samples might be underestimated, and its species might not be precisely determined when using alkyl halide agents for its analysis. To calculate to the extent of this underestimation, further studies are still required.
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spelling pubmed-94052192022-08-26 A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species Wu, Xiaohua Xin, Yuping Wang, Qingda Xia, Yongzhen Xun, Luying Liu, Huaiwei Antioxidants (Basel) Article Using alkyl halides to tag reactive sulfur species (RSSs) (H(2)S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this supposition is facing a challenge. RSS also initiates a reductive dehalogenation reaction, which generates the reduced unloaded tag and oxidized RSS. Therefore, RSS content in bio-samples might be underestimated, and its species might not be precisely determined when using alkyl halide agents for its analysis. To calculate to the extent of this underestimation, further studies are still required. MDPI 2022-08-16 /pmc/articles/PMC9405219/ /pubmed/36009302 http://dx.doi.org/10.3390/antiox11081583 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Xiaohua
Xin, Yuping
Wang, Qingda
Xia, Yongzhen
Xun, Luying
Liu, Huaiwei
A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
title A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
title_full A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
title_fullStr A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
title_full_unstemmed A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
title_short A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
title_sort caveat when using alkyl halides as tagging agents to detect/quantify reactive sulfur species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405219/
https://www.ncbi.nlm.nih.gov/pubmed/36009302
http://dx.doi.org/10.3390/antiox11081583
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