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Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata

A phlorotannin extract was obtained from Himanthalia elongata, revealing a profile rich in fucophlorethol-type and carmalol-type compounds. When subjected to simulated gastrointestinal digestion, its levels of total phlorotannins and antioxidant activity, measured in vitro via NO(●) and O(2)(●−) sca...

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Autores principales: Catarino, Marcelo D., Circuncisão, Ana Rita, Neves, Bruno, Marçal, Catarina, Silva, Artur M. S., Cruz, Maria Teresa, Cardoso, Susana M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405269/
https://www.ncbi.nlm.nih.gov/pubmed/36009238
http://dx.doi.org/10.3390/antiox11081518
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author Catarino, Marcelo D.
Circuncisão, Ana Rita
Neves, Bruno
Marçal, Catarina
Silva, Artur M. S.
Cruz, Maria Teresa
Cardoso, Susana M.
author_facet Catarino, Marcelo D.
Circuncisão, Ana Rita
Neves, Bruno
Marçal, Catarina
Silva, Artur M. S.
Cruz, Maria Teresa
Cardoso, Susana M.
author_sort Catarino, Marcelo D.
collection PubMed
description A phlorotannin extract was obtained from Himanthalia elongata, revealing a profile rich in fucophlorethol-type and carmalol-type compounds. When subjected to simulated gastrointestinal digestion, its levels of total phlorotannins and antioxidant activity, measured in vitro via NO(●) and O(2)(●−) scavenging assays, were reduced, thus suggesting that these compounds’ integrity and bioactivity are negatively affected by the digestive process. Nevertheless, when undigested vs. digested extracts were used on lipopolysaccharide-stimulated Raw 264.7 macrophages, both showed a strong inhibitory effect on the cellular NO(●) production. In fact, although not statistically significant, the digested extract revealed a tendentially stronger effect compared to its undigested counterpart, suggesting that even though there is a decrease in the phlorotannins’ concentration after digestion, with a consequent loss of their scavenging properties, the possible degradation products being formed may exert their effects through the modulation of the intracellular signaling mechanisms. Overall, this study not only contributes to a better understanding of the phlorotannins’ composition of the species H. elongata, but also shows that, although the digestive process may affect the integrity and concentration of these compounds, this does not necessarily translate into loss of bioactivity, in particular the anti-inflammatory activity, probably owing to the bioactive effects that the degradation products of these phenolics may have at an intracellular level.
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spelling pubmed-94052692022-08-26 Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata Catarino, Marcelo D. Circuncisão, Ana Rita Neves, Bruno Marçal, Catarina Silva, Artur M. S. Cruz, Maria Teresa Cardoso, Susana M. Antioxidants (Basel) Article A phlorotannin extract was obtained from Himanthalia elongata, revealing a profile rich in fucophlorethol-type and carmalol-type compounds. When subjected to simulated gastrointestinal digestion, its levels of total phlorotannins and antioxidant activity, measured in vitro via NO(●) and O(2)(●−) scavenging assays, were reduced, thus suggesting that these compounds’ integrity and bioactivity are negatively affected by the digestive process. Nevertheless, when undigested vs. digested extracts were used on lipopolysaccharide-stimulated Raw 264.7 macrophages, both showed a strong inhibitory effect on the cellular NO(●) production. In fact, although not statistically significant, the digested extract revealed a tendentially stronger effect compared to its undigested counterpart, suggesting that even though there is a decrease in the phlorotannins’ concentration after digestion, with a consequent loss of their scavenging properties, the possible degradation products being formed may exert their effects through the modulation of the intracellular signaling mechanisms. Overall, this study not only contributes to a better understanding of the phlorotannins’ composition of the species H. elongata, but also shows that, although the digestive process may affect the integrity and concentration of these compounds, this does not necessarily translate into loss of bioactivity, in particular the anti-inflammatory activity, probably owing to the bioactive effects that the degradation products of these phenolics may have at an intracellular level. MDPI 2022-08-04 /pmc/articles/PMC9405269/ /pubmed/36009238 http://dx.doi.org/10.3390/antiox11081518 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Catarino, Marcelo D.
Circuncisão, Ana Rita
Neves, Bruno
Marçal, Catarina
Silva, Artur M. S.
Cruz, Maria Teresa
Cardoso, Susana M.
Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
title Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
title_full Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
title_fullStr Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
title_full_unstemmed Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
title_short Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
title_sort impact of gastrointestinal digestion on the anti-inflammatory properties of phlorotannins from himanthalia elongata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405269/
https://www.ncbi.nlm.nih.gov/pubmed/36009238
http://dx.doi.org/10.3390/antiox11081518
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