Cargando…
Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation
Whitlockite (WH) is a calcium-phosphate-based Mg-containing ceramic with good mechanical properties, rapid resorption, and good osteogenicity. Recently, we successfully synthesized highly porous WH granules using a marine plankton exoskeleton (MP-WH). In the present study, we improved the osteoinduc...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405279/ https://www.ncbi.nlm.nih.gov/pubmed/36004923 http://dx.doi.org/10.3390/bioengineering9080399 |
_version_ | 1784773842027151360 |
---|---|
author | Baek, Ji Won Kim, Ki Su Park, Ho Park, Nak Gyu Kim, Beom-Su |
author_facet | Baek, Ji Won Kim, Ki Su Park, Ho Park, Nak Gyu Kim, Beom-Su |
author_sort | Baek, Ji Won |
collection | PubMed |
description | Whitlockite (WH) is a calcium-phosphate-based Mg-containing ceramic with good mechanical properties, rapid resorption, and good osteogenicity. Recently, we successfully synthesized highly porous WH granules using a marine plankton exoskeleton (MP-WH). In the present study, we improved the osteoinductive activity of MP-WH granules by bone morphogenetic protein2 (BMP2) (MP-WH/BMP2). The surface morphology and composition of the fabricated MP-WH/BMP2 granules were characterized using scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared (FT-IR) spectroscopy. The biocompatibility and osteogenic effects were evaluated using human mesenchymal stem cells (hMSCs). BMP2 was absorbed on the surfaces of the MP-WH/BMP2 granules. Immobilized BMP2 was released at a moderate rate over 30 days. hMSCs seeded on MP-WH/BMP2 granules became biocompatible, with a better proliferation and adhesion for MP-WH/BMP2, compared with MP-WH. Bone-specific markers Runx2, type I collagen, osteocalcin, and osteopontin were significantly upregulated following BMP2 incorporation. Similar observations were made regarding the alkaline phosphatase activity. This study suggests that BMP2 incorporation improves the osteoinductive activity of marine-plankton-derived WH granules for bone tissue repair. |
format | Online Article Text |
id | pubmed-9405279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94052792022-08-26 Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation Baek, Ji Won Kim, Ki Su Park, Ho Park, Nak Gyu Kim, Beom-Su Bioengineering (Basel) Article Whitlockite (WH) is a calcium-phosphate-based Mg-containing ceramic with good mechanical properties, rapid resorption, and good osteogenicity. Recently, we successfully synthesized highly porous WH granules using a marine plankton exoskeleton (MP-WH). In the present study, we improved the osteoinductive activity of MP-WH granules by bone morphogenetic protein2 (BMP2) (MP-WH/BMP2). The surface morphology and composition of the fabricated MP-WH/BMP2 granules were characterized using scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared (FT-IR) spectroscopy. The biocompatibility and osteogenic effects were evaluated using human mesenchymal stem cells (hMSCs). BMP2 was absorbed on the surfaces of the MP-WH/BMP2 granules. Immobilized BMP2 was released at a moderate rate over 30 days. hMSCs seeded on MP-WH/BMP2 granules became biocompatible, with a better proliferation and adhesion for MP-WH/BMP2, compared with MP-WH. Bone-specific markers Runx2, type I collagen, osteocalcin, and osteopontin were significantly upregulated following BMP2 incorporation. Similar observations were made regarding the alkaline phosphatase activity. This study suggests that BMP2 incorporation improves the osteoinductive activity of marine-plankton-derived WH granules for bone tissue repair. MDPI 2022-08-17 /pmc/articles/PMC9405279/ /pubmed/36004923 http://dx.doi.org/10.3390/bioengineering9080399 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baek, Ji Won Kim, Ki Su Park, Ho Park, Nak Gyu Kim, Beom-Su Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
title | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
title_full | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
title_fullStr | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
title_full_unstemmed | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
title_short | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
title_sort | enhanced biocompatibility and osteogenic activity of marine-plankton-derived whitlockite bone granules through bone morphogenetic protein 2 incorporation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405279/ https://www.ncbi.nlm.nih.gov/pubmed/36004923 http://dx.doi.org/10.3390/bioengineering9080399 |
work_keys_str_mv | AT baekjiwon enhancedbiocompatibilityandosteogenicactivityofmarineplanktonderivedwhitlockitebonegranulesthroughbonemorphogeneticprotein2incorporation AT kimkisu enhancedbiocompatibilityandosteogenicactivityofmarineplanktonderivedwhitlockitebonegranulesthroughbonemorphogeneticprotein2incorporation AT parkho enhancedbiocompatibilityandosteogenicactivityofmarineplanktonderivedwhitlockitebonegranulesthroughbonemorphogeneticprotein2incorporation AT parknakgyu enhancedbiocompatibilityandosteogenicactivityofmarineplanktonderivedwhitlockitebonegranulesthroughbonemorphogeneticprotein2incorporation AT kimbeomsu enhancedbiocompatibilityandosteogenicactivityofmarineplanktonderivedwhitlockitebonegranulesthroughbonemorphogeneticprotein2incorporation |