Cargando…

Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids

Aeromonas salmonicida subsp. salmonicida is a pathogenic bacterium responsible for furunculosis in salmonids. Following an outbreak of furunculosis, the infection can be treated with antibiotics, but it is common to observe ineffective treatment due to antibiotic resistance. This bacterium has a wid...

Descripción completa

Detalles Bibliográficos
Autores principales: Fournier, Kim C., Paquet, Valérie E., Attéré, Sabrina A., Farley, Judith, Marquis, Hélène, Gantelet, Hubert, Ravaille, Christian, Vincent, Antony T., Charette, Steve J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405359/
https://www.ncbi.nlm.nih.gov/pubmed/36009916
http://dx.doi.org/10.3390/antibiotics11081047
_version_ 1784773861562122240
author Fournier, Kim C.
Paquet, Valérie E.
Attéré, Sabrina A.
Farley, Judith
Marquis, Hélène
Gantelet, Hubert
Ravaille, Christian
Vincent, Antony T.
Charette, Steve J.
author_facet Fournier, Kim C.
Paquet, Valérie E.
Attéré, Sabrina A.
Farley, Judith
Marquis, Hélène
Gantelet, Hubert
Ravaille, Christian
Vincent, Antony T.
Charette, Steve J.
author_sort Fournier, Kim C.
collection PubMed
description Aeromonas salmonicida subsp. salmonicida is a pathogenic bacterium responsible for furunculosis in salmonids. Following an outbreak of furunculosis, the infection can be treated with antibiotics, but it is common to observe ineffective treatment due to antibiotic resistance. This bacterium has a wide variety of plasmids responsible for this resistance. Among them, pRAS3 carries a tetracycline resistance gene. Several variants of this plasmid have been discovered over the years (pRAS3-3432 and pRAS3.1 to 3.4). During the present study, two new variants of the plasmid pRAS3 were identified (pRAS3.5 and pRAS3-3759) in strains of A. salmonicida subsp. salmonicida. Plasmid pRAS3-3759, which has been found in many strains from the same region over the past three years, has an additional genetic element identical to one found in pRAS3-3432. This genetic element was also found in Chlamydia suis, a swine pathogen. In this study, we analyzed the bacteria’s resistance to tetracycline, the number of copies of the plasmids, and the growth of the strains that carry five of the pRAS3 variants (pRAS3.3 to 3.5, pRAS3-3432, and pRAS3-3759). The results show no particular trend despite the differences between the plasmids, except for the resistance to tetracycline when analyzed in an isogenic background. Blast analysis also revealed the presence of pRAS3 plasmids in other bacterial species, which suggests that this plasmid family has widely spread. This study once again highlights the ability of A. salmonicida subsp. salmonicida to adapt to furunculosis antibiotic treatments, and the still-growing family of pRAS3 plasmids.
format Online
Article
Text
id pubmed-9405359
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94053592022-08-26 Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids Fournier, Kim C. Paquet, Valérie E. Attéré, Sabrina A. Farley, Judith Marquis, Hélène Gantelet, Hubert Ravaille, Christian Vincent, Antony T. Charette, Steve J. Antibiotics (Basel) Article Aeromonas salmonicida subsp. salmonicida is a pathogenic bacterium responsible for furunculosis in salmonids. Following an outbreak of furunculosis, the infection can be treated with antibiotics, but it is common to observe ineffective treatment due to antibiotic resistance. This bacterium has a wide variety of plasmids responsible for this resistance. Among them, pRAS3 carries a tetracycline resistance gene. Several variants of this plasmid have been discovered over the years (pRAS3-3432 and pRAS3.1 to 3.4). During the present study, two new variants of the plasmid pRAS3 were identified (pRAS3.5 and pRAS3-3759) in strains of A. salmonicida subsp. salmonicida. Plasmid pRAS3-3759, which has been found in many strains from the same region over the past three years, has an additional genetic element identical to one found in pRAS3-3432. This genetic element was also found in Chlamydia suis, a swine pathogen. In this study, we analyzed the bacteria’s resistance to tetracycline, the number of copies of the plasmids, and the growth of the strains that carry five of the pRAS3 variants (pRAS3.3 to 3.5, pRAS3-3432, and pRAS3-3759). The results show no particular trend despite the differences between the plasmids, except for the resistance to tetracycline when analyzed in an isogenic background. Blast analysis also revealed the presence of pRAS3 plasmids in other bacterial species, which suggests that this plasmid family has widely spread. This study once again highlights the ability of A. salmonicida subsp. salmonicida to adapt to furunculosis antibiotic treatments, and the still-growing family of pRAS3 plasmids. MDPI 2022-08-03 /pmc/articles/PMC9405359/ /pubmed/36009916 http://dx.doi.org/10.3390/antibiotics11081047 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fournier, Kim C.
Paquet, Valérie E.
Attéré, Sabrina A.
Farley, Judith
Marquis, Hélène
Gantelet, Hubert
Ravaille, Christian
Vincent, Antony T.
Charette, Steve J.
Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids
title Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids
title_full Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids
title_fullStr Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids
title_full_unstemmed Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids
title_short Expansion of the pRAS3 Plasmid Family in Aeromonas salmonicida subsp. salmonicida and Growing Evidence of Interspecies Connections for These Plasmids
title_sort expansion of the pras3 plasmid family in aeromonas salmonicida subsp. salmonicida and growing evidence of interspecies connections for these plasmids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405359/
https://www.ncbi.nlm.nih.gov/pubmed/36009916
http://dx.doi.org/10.3390/antibiotics11081047
work_keys_str_mv AT fournierkimc expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT paquetvaleriee expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT atteresabrinaa expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT farleyjudith expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT marquishelene expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT gantelethubert expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT ravaillechristian expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT vincentantonyt expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids
AT charettestevej expansionofthepras3plasmidfamilyinaeromonassalmonicidasubspsalmonicidaandgrowingevidenceofinterspeciesconnectionsfortheseplasmids