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Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity

Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genome-based...

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Autores principales: Ashraf, Neelma, Zafar, Sana, Makitrynskyy, Roman, Bechthold, Andreas, Spiteller, Dieter, Song, Lijiang, Anwar, Munir Ahmad, Luzhetskyy, Andriy, Khan, Ali Nisar, Akhtar, Kalsoom, Khaliq, Shazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405382/
https://www.ncbi.nlm.nih.gov/pubmed/36009926
http://dx.doi.org/10.3390/antibiotics11081057
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author Ashraf, Neelma
Zafar, Sana
Makitrynskyy, Roman
Bechthold, Andreas
Spiteller, Dieter
Song, Lijiang
Anwar, Munir Ahmad
Luzhetskyy, Andriy
Khan, Ali Nisar
Akhtar, Kalsoom
Khaliq, Shazia
author_facet Ashraf, Neelma
Zafar, Sana
Makitrynskyy, Roman
Bechthold, Andreas
Spiteller, Dieter
Song, Lijiang
Anwar, Munir Ahmad
Luzhetskyy, Andriy
Khan, Ali Nisar
Akhtar, Kalsoom
Khaliq, Shazia
author_sort Ashraf, Neelma
collection PubMed
description Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genome-based evaluation of Streptomyces sp. isolate BR123 was analyzed to determine its biosynthetic potential, based on its in vitro antimicrobial activity against a broad range of microbial pathogens, including gram-positive and gram-negative bacteria and fungi. A draft genome sequence of 8.15 Mb of Streptomyces sp. isolate BR123 was attained, containing a GC content of 72.63% and 8103 protein coding genes. Many antimicrobial, antiparasitic, and anticancerous compounds were detected by the presence of multiple biosynthetic gene clusters, which was predicted by in silico analysis. A novel metabolite with a molecular mass of 1271.7773 in positive ion mode was detected through a high-performance liquid chromatography linked with mass spectrometry (HPLC-MS) analysis. In addition, another compound, meridamycin, was also identified through a HPLC-MS analysis. The current study reveals the biosynthetic potential of Streptomyces sp. isolate BR123, with respect to the synthesis of bioactive secondary metabolites through genomic and spectrometric analysis. Moreover, the comparative genome study compared the isolate BR123 with other Streptomyces strains, which may expand the knowledge concerning the mechanism involved in novel antimicrobial metabolite synthesis.
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spelling pubmed-94053822022-08-26 Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity Ashraf, Neelma Zafar, Sana Makitrynskyy, Roman Bechthold, Andreas Spiteller, Dieter Song, Lijiang Anwar, Munir Ahmad Luzhetskyy, Andriy Khan, Ali Nisar Akhtar, Kalsoom Khaliq, Shazia Antibiotics (Basel) Article Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genome-based evaluation of Streptomyces sp. isolate BR123 was analyzed to determine its biosynthetic potential, based on its in vitro antimicrobial activity against a broad range of microbial pathogens, including gram-positive and gram-negative bacteria and fungi. A draft genome sequence of 8.15 Mb of Streptomyces sp. isolate BR123 was attained, containing a GC content of 72.63% and 8103 protein coding genes. Many antimicrobial, antiparasitic, and anticancerous compounds were detected by the presence of multiple biosynthetic gene clusters, which was predicted by in silico analysis. A novel metabolite with a molecular mass of 1271.7773 in positive ion mode was detected through a high-performance liquid chromatography linked with mass spectrometry (HPLC-MS) analysis. In addition, another compound, meridamycin, was also identified through a HPLC-MS analysis. The current study reveals the biosynthetic potential of Streptomyces sp. isolate BR123, with respect to the synthesis of bioactive secondary metabolites through genomic and spectrometric analysis. Moreover, the comparative genome study compared the isolate BR123 with other Streptomyces strains, which may expand the knowledge concerning the mechanism involved in novel antimicrobial metabolite synthesis. MDPI 2022-08-04 /pmc/articles/PMC9405382/ /pubmed/36009926 http://dx.doi.org/10.3390/antibiotics11081057 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ashraf, Neelma
Zafar, Sana
Makitrynskyy, Roman
Bechthold, Andreas
Spiteller, Dieter
Song, Lijiang
Anwar, Munir Ahmad
Luzhetskyy, Andriy
Khan, Ali Nisar
Akhtar, Kalsoom
Khaliq, Shazia
Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
title Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
title_full Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
title_fullStr Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
title_full_unstemmed Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
title_short Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
title_sort revealing genome-based biosynthetic potential of streptomyces sp. br123 isolated from sunflower rhizosphere with broad spectrum antimicrobial activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405382/
https://www.ncbi.nlm.nih.gov/pubmed/36009926
http://dx.doi.org/10.3390/antibiotics11081057
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