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On the accuracy of cell-attached current-clamp recordings from cortical neurons

Cell-attached current-clamp (CA/CC) recordings have been proposed to measure resting membrane potential and synaptic/agonist responses in neurons without disrupting the cell membrane, thus avoiding the intracellular dialysis that occurs in conventional whole-cell recordings (WC). However, the accura...

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Autores principales: Vazetdinova, Alina, Valiullina-Rakhmatullina, Fliza, Rozov, Andrei, Evstifeev, Alexander, Khazipov, Roustem, Nasretdinov, Azat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405422/
https://www.ncbi.nlm.nih.gov/pubmed/36034500
http://dx.doi.org/10.3389/fnmol.2022.979479
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author Vazetdinova, Alina
Valiullina-Rakhmatullina, Fliza
Rozov, Andrei
Evstifeev, Alexander
Khazipov, Roustem
Nasretdinov, Azat
author_facet Vazetdinova, Alina
Valiullina-Rakhmatullina, Fliza
Rozov, Andrei
Evstifeev, Alexander
Khazipov, Roustem
Nasretdinov, Azat
author_sort Vazetdinova, Alina
collection PubMed
description Cell-attached current-clamp (CA/CC) recordings have been proposed to measure resting membrane potential and synaptic/agonist responses in neurons without disrupting the cell membrane, thus avoiding the intracellular dialysis that occurs in conventional whole-cell recordings (WC). However, the accuracy of CA/CC recordings in neurons has not been directly assessed. Here, we used concomitant CA and WC current clamp recordings from cortical neurons in brain slices. Resting membrane potential values and slow voltage shifts showed variability and were typically attenuated during CA/CC recordings by ~10–20% relative to WC values. Fast signals were slowed down and their amplitude was greatly reduced: synaptic potentials by nearly 2-fold, and action potentials by nearly 10-fold in CA/CC mode compared to WC. The polarity of GABAergic postsynaptic responses in CA/CC mode matched the responses in WC, and depolarising GABAergic potentials were predominantly observed during CA/CC recordings of intact neonatal CA3 hippocampal pyramidal neurons. Similarly, CA/CC recordings reliably detected neuronal depolarization and excitation during network-induced giant depolarizing potentials in the neonatal CA3 hippocampus, and revealed variable changes, from depolarization to hyperpolarization, in CA1 pyramidal cells during sharp wave ripples in the adult hippocampus. Thus, CA/CC recordings are suitable for assessing membrane potential but signal distortion, probably caused by leakage via the seal contact and RC filtering should be considered.
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spelling pubmed-94054222022-08-26 On the accuracy of cell-attached current-clamp recordings from cortical neurons Vazetdinova, Alina Valiullina-Rakhmatullina, Fliza Rozov, Andrei Evstifeev, Alexander Khazipov, Roustem Nasretdinov, Azat Front Mol Neurosci Molecular Neuroscience Cell-attached current-clamp (CA/CC) recordings have been proposed to measure resting membrane potential and synaptic/agonist responses in neurons without disrupting the cell membrane, thus avoiding the intracellular dialysis that occurs in conventional whole-cell recordings (WC). However, the accuracy of CA/CC recordings in neurons has not been directly assessed. Here, we used concomitant CA and WC current clamp recordings from cortical neurons in brain slices. Resting membrane potential values and slow voltage shifts showed variability and were typically attenuated during CA/CC recordings by ~10–20% relative to WC values. Fast signals were slowed down and their amplitude was greatly reduced: synaptic potentials by nearly 2-fold, and action potentials by nearly 10-fold in CA/CC mode compared to WC. The polarity of GABAergic postsynaptic responses in CA/CC mode matched the responses in WC, and depolarising GABAergic potentials were predominantly observed during CA/CC recordings of intact neonatal CA3 hippocampal pyramidal neurons. Similarly, CA/CC recordings reliably detected neuronal depolarization and excitation during network-induced giant depolarizing potentials in the neonatal CA3 hippocampus, and revealed variable changes, from depolarization to hyperpolarization, in CA1 pyramidal cells during sharp wave ripples in the adult hippocampus. Thus, CA/CC recordings are suitable for assessing membrane potential but signal distortion, probably caused by leakage via the seal contact and RC filtering should be considered. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9405422/ /pubmed/36034500 http://dx.doi.org/10.3389/fnmol.2022.979479 Text en Copyright © 2022 Vazetdinova, Valiullina-Rakhmatullina, Rozov, Evstifeev, Khazipov and Nasretdinov. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Vazetdinova, Alina
Valiullina-Rakhmatullina, Fliza
Rozov, Andrei
Evstifeev, Alexander
Khazipov, Roustem
Nasretdinov, Azat
On the accuracy of cell-attached current-clamp recordings from cortical neurons
title On the accuracy of cell-attached current-clamp recordings from cortical neurons
title_full On the accuracy of cell-attached current-clamp recordings from cortical neurons
title_fullStr On the accuracy of cell-attached current-clamp recordings from cortical neurons
title_full_unstemmed On the accuracy of cell-attached current-clamp recordings from cortical neurons
title_short On the accuracy of cell-attached current-clamp recordings from cortical neurons
title_sort on the accuracy of cell-attached current-clamp recordings from cortical neurons
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405422/
https://www.ncbi.nlm.nih.gov/pubmed/36034500
http://dx.doi.org/10.3389/fnmol.2022.979479
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