Derivation and culturing of neural stem cells from human embryonic brain tissue

Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We desc...

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Autores principales: Suk, Yujin, Kieliszek, Agata, Mobilio, Daniel, Venugopal, Chitra, Singh, Sheila K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405532/
https://www.ncbi.nlm.nih.gov/pubmed/36035806
http://dx.doi.org/10.1016/j.xpro.2022.101628
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author Suk, Yujin
Kieliszek, Agata
Mobilio, Daniel
Venugopal, Chitra
Singh, Sheila K.
author_facet Suk, Yujin
Kieliszek, Agata
Mobilio, Daniel
Venugopal, Chitra
Singh, Sheila K.
author_sort Suk, Yujin
collection PubMed
description Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).
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spelling pubmed-94055322022-08-26 Derivation and culturing of neural stem cells from human embryonic brain tissue Suk, Yujin Kieliszek, Agata Mobilio, Daniel Venugopal, Chitra Singh, Sheila K. STAR Protoc Protocol Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a). Elsevier 2022-08-18 /pmc/articles/PMC9405532/ /pubmed/36035806 http://dx.doi.org/10.1016/j.xpro.2022.101628 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Suk, Yujin
Kieliszek, Agata
Mobilio, Daniel
Venugopal, Chitra
Singh, Sheila K.
Derivation and culturing of neural stem cells from human embryonic brain tissue
title Derivation and culturing of neural stem cells from human embryonic brain tissue
title_full Derivation and culturing of neural stem cells from human embryonic brain tissue
title_fullStr Derivation and culturing of neural stem cells from human embryonic brain tissue
title_full_unstemmed Derivation and culturing of neural stem cells from human embryonic brain tissue
title_short Derivation and culturing of neural stem cells from human embryonic brain tissue
title_sort derivation and culturing of neural stem cells from human embryonic brain tissue
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405532/
https://www.ncbi.nlm.nih.gov/pubmed/36035806
http://dx.doi.org/10.1016/j.xpro.2022.101628
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