Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO

Visualization of mRNA molecules in single cells has revealed their core mechanisms as well as sources of cell-to-cell and spatiotemporal heterogeneity. Here, we describe a protocol to label, visualize, and quantify mRNA molecules by time-lapse imaging with the capability of resolving mRNA molecules...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Yue, Kowalczyk, Gabriel J., Lee, Robin E.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405536/
https://www.ncbi.nlm.nih.gov/pubmed/36035802
http://dx.doi.org/10.1016/j.xpro.2022.101630
_version_ 1784773902264696832
author Guo, Yue
Kowalczyk, Gabriel J.
Lee, Robin E.C.
author_facet Guo, Yue
Kowalczyk, Gabriel J.
Lee, Robin E.C.
author_sort Guo, Yue
collection PubMed
description Visualization of mRNA molecules in single cells has revealed their core mechanisms as well as sources of cell-to-cell and spatiotemporal heterogeneity. Here, we describe a protocol to label, visualize, and quantify mRNA molecules by time-lapse imaging with the capability of resolving mRNA molecules over durations of hours to days. We provide links to mRNA-labeling plasmids as well as free software for a semi-automated image analysis pipeline. For complete details on the use and execution of this protocol, please refer to Guo and Lee (2022) and Kowalczyk et al. (2021).
format Online
Article
Text
id pubmed-9405536
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-94055362022-08-26 Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO Guo, Yue Kowalczyk, Gabriel J. Lee, Robin E.C. STAR Protoc Protocol Visualization of mRNA molecules in single cells has revealed their core mechanisms as well as sources of cell-to-cell and spatiotemporal heterogeneity. Here, we describe a protocol to label, visualize, and quantify mRNA molecules by time-lapse imaging with the capability of resolving mRNA molecules over durations of hours to days. We provide links to mRNA-labeling plasmids as well as free software for a semi-automated image analysis pipeline. For complete details on the use and execution of this protocol, please refer to Guo and Lee (2022) and Kowalczyk et al. (2021). Elsevier 2022-08-18 /pmc/articles/PMC9405536/ /pubmed/36035802 http://dx.doi.org/10.1016/j.xpro.2022.101630 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Guo, Yue
Kowalczyk, Gabriel J.
Lee, Robin E.C.
Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
title Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
title_full Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
title_fullStr Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
title_full_unstemmed Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
title_short Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
title_sort label and quantify mrna molecules in live cell experiments using sunriser and dnemo
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405536/
https://www.ncbi.nlm.nih.gov/pubmed/36035802
http://dx.doi.org/10.1016/j.xpro.2022.101630
work_keys_str_mv AT guoyue labelandquantifymrnamoleculesinlivecellexperimentsusingsunriseranddnemo
AT kowalczykgabrielj labelandquantifymrnamoleculesinlivecellexperimentsusingsunriseranddnemo
AT leerobinec labelandquantifymrnamoleculesinlivecellexperimentsusingsunriseranddnemo

Ejemplares similares