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Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact t...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405971/ https://www.ncbi.nlm.nih.gov/pubmed/36008977 http://dx.doi.org/10.3390/biom12081083 |
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author | Kumar, Mukesh Has, Canan Lam-Kamath, Khanh Ayciriex, Sophie Dewett, Deepshe Bashir, Mhamed Poupault, Clara Schuhmann, Kai Knittelfelder, Oskar Raghuraman, Bharath Kumar Ahrends, Robert Rister, Jens Shevchenko, Andrej |
author_facet | Kumar, Mukesh Has, Canan Lam-Kamath, Khanh Ayciriex, Sophie Dewett, Deepshe Bashir, Mhamed Poupault, Clara Schuhmann, Kai Knittelfelder, Oskar Raghuraman, Bharath Kumar Ahrends, Robert Rister, Jens Shevchenko, Andrej |
author_sort | Kumar, Mukesh |
collection | PubMed |
description | The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated. |
format | Online Article Text |
id | pubmed-9405971 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94059712022-08-26 Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome Kumar, Mukesh Has, Canan Lam-Kamath, Khanh Ayciriex, Sophie Dewett, Deepshe Bashir, Mhamed Poupault, Clara Schuhmann, Kai Knittelfelder, Oskar Raghuraman, Bharath Kumar Ahrends, Robert Rister, Jens Shevchenko, Andrej Biomolecules Article The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated. MDPI 2022-08-06 /pmc/articles/PMC9405971/ /pubmed/36008977 http://dx.doi.org/10.3390/biom12081083 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kumar, Mukesh Has, Canan Lam-Kamath, Khanh Ayciriex, Sophie Dewett, Deepshe Bashir, Mhamed Poupault, Clara Schuhmann, Kai Knittelfelder, Oskar Raghuraman, Bharath Kumar Ahrends, Robert Rister, Jens Shevchenko, Andrej Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome |
title | Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome |
title_full | Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome |
title_fullStr | Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome |
title_full_unstemmed | Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome |
title_short | Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome |
title_sort | vitamin a deficiency alters the phototransduction machinery and distinct non-vision-specific pathways in the drosophila eye proteome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405971/ https://www.ncbi.nlm.nih.gov/pubmed/36008977 http://dx.doi.org/10.3390/biom12081083 |
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