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Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome

The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact t...

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Autores principales: Kumar, Mukesh, Has, Canan, Lam-Kamath, Khanh, Ayciriex, Sophie, Dewett, Deepshe, Bashir, Mhamed, Poupault, Clara, Schuhmann, Kai, Knittelfelder, Oskar, Raghuraman, Bharath Kumar, Ahrends, Robert, Rister, Jens, Shevchenko, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405971/
https://www.ncbi.nlm.nih.gov/pubmed/36008977
http://dx.doi.org/10.3390/biom12081083
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author Kumar, Mukesh
Has, Canan
Lam-Kamath, Khanh
Ayciriex, Sophie
Dewett, Deepshe
Bashir, Mhamed
Poupault, Clara
Schuhmann, Kai
Knittelfelder, Oskar
Raghuraman, Bharath Kumar
Ahrends, Robert
Rister, Jens
Shevchenko, Andrej
author_facet Kumar, Mukesh
Has, Canan
Lam-Kamath, Khanh
Ayciriex, Sophie
Dewett, Deepshe
Bashir, Mhamed
Poupault, Clara
Schuhmann, Kai
Knittelfelder, Oskar
Raghuraman, Bharath Kumar
Ahrends, Robert
Rister, Jens
Shevchenko, Andrej
author_sort Kumar, Mukesh
collection PubMed
description The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated.
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spelling pubmed-94059712022-08-26 Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome Kumar, Mukesh Has, Canan Lam-Kamath, Khanh Ayciriex, Sophie Dewett, Deepshe Bashir, Mhamed Poupault, Clara Schuhmann, Kai Knittelfelder, Oskar Raghuraman, Bharath Kumar Ahrends, Robert Rister, Jens Shevchenko, Andrej Biomolecules Article The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated. MDPI 2022-08-06 /pmc/articles/PMC9405971/ /pubmed/36008977 http://dx.doi.org/10.3390/biom12081083 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kumar, Mukesh
Has, Canan
Lam-Kamath, Khanh
Ayciriex, Sophie
Dewett, Deepshe
Bashir, Mhamed
Poupault, Clara
Schuhmann, Kai
Knittelfelder, Oskar
Raghuraman, Bharath Kumar
Ahrends, Robert
Rister, Jens
Shevchenko, Andrej
Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
title Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
title_full Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
title_fullStr Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
title_full_unstemmed Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
title_short Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
title_sort vitamin a deficiency alters the phototransduction machinery and distinct non-vision-specific pathways in the drosophila eye proteome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405971/
https://www.ncbi.nlm.nih.gov/pubmed/36008977
http://dx.doi.org/10.3390/biom12081083
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