Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells

Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, strom...

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Autores principales: Cid-Diaz, Tania, Lodeiro, Andrea C., Duran, Angeles, Moscat, Jorge, Diaz-Meco, Maria T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405995/
https://www.ncbi.nlm.nih.gov/pubmed/36035805
http://dx.doi.org/10.1016/j.xpro.2022.101635
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author Cid-Diaz, Tania
Lodeiro, Andrea C.
Duran, Angeles
Moscat, Jorge
Diaz-Meco, Maria T.
author_facet Cid-Diaz, Tania
Lodeiro, Andrea C.
Duran, Angeles
Moscat, Jorge
Diaz-Meco, Maria T.
author_sort Cid-Diaz, Tania
collection PubMed
description Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, stromal components, or ECM composition. Here, we detail the steps to build an organotypic culture including the preparation of the organotypic structure, organotypic gels, cell seeding, gel casting, membrane processing, and image and data analysis. For complete details on the use and execution of this protocol, please refer to Linares et al. (2022).
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spelling pubmed-94059952022-08-26 Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells Cid-Diaz, Tania Lodeiro, Andrea C. Duran, Angeles Moscat, Jorge Diaz-Meco, Maria T. STAR Protoc Protocol Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, stromal components, or ECM composition. Here, we detail the steps to build an organotypic culture including the preparation of the organotypic structure, organotypic gels, cell seeding, gel casting, membrane processing, and image and data analysis. For complete details on the use and execution of this protocol, please refer to Linares et al. (2022). Elsevier 2022-08-18 /pmc/articles/PMC9405995/ /pubmed/36035805 http://dx.doi.org/10.1016/j.xpro.2022.101635 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Cid-Diaz, Tania
Lodeiro, Andrea C.
Duran, Angeles
Moscat, Jorge
Diaz-Meco, Maria T.
Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
title Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
title_full Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
title_fullStr Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
title_full_unstemmed Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
title_short Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
title_sort air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405995/
https://www.ncbi.nlm.nih.gov/pubmed/36035805
http://dx.doi.org/10.1016/j.xpro.2022.101635
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