Cargando…
The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells
Autologous lipotransfer is a promising method for tissue regeneration, because white adipose tissue contains a heterogeneous cell population, including mesenchymal stem cells, endothelial cells, immune cells, and adipocytes. In order to improve the outcome, adipose tissue can be processed before app...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406387/ https://www.ncbi.nlm.nih.gov/pubmed/36010620 http://dx.doi.org/10.3390/cells11162543 |
_version_ | 1784774108505964544 |
---|---|
author | Eigenberger, Andreas Felthaus, Oliver Schratzenstaller, Thomas Haerteis, Silke Utpatel, Kirsten Prantl, Lukas |
author_facet | Eigenberger, Andreas Felthaus, Oliver Schratzenstaller, Thomas Haerteis, Silke Utpatel, Kirsten Prantl, Lukas |
author_sort | Eigenberger, Andreas |
collection | PubMed |
description | Autologous lipotransfer is a promising method for tissue regeneration, because white adipose tissue contains a heterogeneous cell population, including mesenchymal stem cells, endothelial cells, immune cells, and adipocytes. In order to improve the outcome, adipose tissue can be processed before application. In this study, we investigated changes caused by mechanical processing. Lipoaspirates were processed using sedimentation, first-time centrifugation, shear-force homogenization, and second-time centrifugation. The average adipocyte size, stromal vascular cell count, and adipocyte depot size were examined histologically at every processing step. In addition, the adipose derived stem cells (ADSCs) were isolated and differentiated osteogenically and adipogenically. While homogenization causes a disruption of adipocyte depots, the shape of the remaining adipocytes is not changed. On average, these adipocytes are smaller than the depot adipocytes, they are surrounded by the ECM, and therefore mechanically more stable. The volume loss of adipocyte depots leads to a significant enrichment of stromal vascular cells such as ADSCs. However, the mechanical processing does not change the potential of the ADSCs to differentiate adipogenically or osteogenically. It thus appears that mechanically processed lipoaspirates are promising for the reparation of even mechanically stressed tissue as that found in nasolabial folds. The changes resulting from the processing correspond more to a filtration of mechanically less stable components than to a manipulation of the tissue. |
format | Online Article Text |
id | pubmed-9406387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94063872022-08-26 The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells Eigenberger, Andreas Felthaus, Oliver Schratzenstaller, Thomas Haerteis, Silke Utpatel, Kirsten Prantl, Lukas Cells Article Autologous lipotransfer is a promising method for tissue regeneration, because white adipose tissue contains a heterogeneous cell population, including mesenchymal stem cells, endothelial cells, immune cells, and adipocytes. In order to improve the outcome, adipose tissue can be processed before application. In this study, we investigated changes caused by mechanical processing. Lipoaspirates were processed using sedimentation, first-time centrifugation, shear-force homogenization, and second-time centrifugation. The average adipocyte size, stromal vascular cell count, and adipocyte depot size were examined histologically at every processing step. In addition, the adipose derived stem cells (ADSCs) were isolated and differentiated osteogenically and adipogenically. While homogenization causes a disruption of adipocyte depots, the shape of the remaining adipocytes is not changed. On average, these adipocytes are smaller than the depot adipocytes, they are surrounded by the ECM, and therefore mechanically more stable. The volume loss of adipocyte depots leads to a significant enrichment of stromal vascular cells such as ADSCs. However, the mechanical processing does not change the potential of the ADSCs to differentiate adipogenically or osteogenically. It thus appears that mechanically processed lipoaspirates are promising for the reparation of even mechanically stressed tissue as that found in nasolabial folds. The changes resulting from the processing correspond more to a filtration of mechanically less stable components than to a manipulation of the tissue. MDPI 2022-08-16 /pmc/articles/PMC9406387/ /pubmed/36010620 http://dx.doi.org/10.3390/cells11162543 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eigenberger, Andreas Felthaus, Oliver Schratzenstaller, Thomas Haerteis, Silke Utpatel, Kirsten Prantl, Lukas The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells |
title | The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells |
title_full | The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells |
title_fullStr | The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells |
title_full_unstemmed | The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells |
title_short | The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells |
title_sort | effects of shear force-based processing of lipoaspirates on white adipose tissue and the differentiation potential of adipose derived stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406387/ https://www.ncbi.nlm.nih.gov/pubmed/36010620 http://dx.doi.org/10.3390/cells11162543 |
work_keys_str_mv | AT eigenbergerandreas theeffectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT felthausoliver theeffectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT schratzenstallerthomas theeffectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT haerteissilke theeffectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT utpatelkirsten theeffectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT prantllukas theeffectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT eigenbergerandreas effectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT felthausoliver effectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT schratzenstallerthomas effectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT haerteissilke effectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT utpatelkirsten effectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells AT prantllukas effectsofshearforcebasedprocessingoflipoaspiratesonwhiteadiposetissueandthedifferentiationpotentialofadiposederivedstemcells |