Cargando…
Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens
Excessive lipid deposition in layer chickens due to inappropriate feeding adversely affects egg production; however, nutritional manipulation methods to deal with this issue are still limited. β-hydroxy-β-methylbutyrate (HMB), a metabolite of L-leucine, was recently reported as a lipid-lowering nutr...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406763/ https://www.ncbi.nlm.nih.gov/pubmed/36005131 http://dx.doi.org/10.3390/cimb44080235 |
_version_ | 1784774200495439872 |
---|---|
author | Liao, Qichao Wu, Tian Fu, Qinghua Wang, Peng Zhao, Yameng Li, Yan Xiao, Haihan Zhou, Lei Song, Ziyi |
author_facet | Liao, Qichao Wu, Tian Fu, Qinghua Wang, Peng Zhao, Yameng Li, Yan Xiao, Haihan Zhou, Lei Song, Ziyi |
author_sort | Liao, Qichao |
collection | PubMed |
description | Excessive lipid deposition in layer chickens due to inappropriate feeding adversely affects egg production; however, nutritional manipulation methods to deal with this issue are still limited. β-hydroxy-β-methylbutyrate (HMB), a metabolite of L-leucine, was recently reported as a lipid-lowering nutrient in mice and pigs, although its role in layers had not been investigated. Here, we employed high-fat and high-cholesterol diet (HFHCD)−challenged growing layers as an obese model to explore HMB function in the regulation of lipid metabolism and the potential mechanisms involved. We found that dietary supplementation with (0.05% or 0.10%) HMB significantly reduced HFHCD−induced bodyweight growth in layers, mainly due to reduction in abdominal fat deposition. Mechanistically, HMB supplementation enhanced hepatic bile acid synthesis from cholesterol through elevating expression of Cyp7a1, a gene coding a key enzyme in bile acid synthesis. Furthermore, 16S rRNA gene sequencing revealed that HMB supplementation remodeled the diversity and composition of the layers’ cecal microbiota, and the abundance of Bacteroidetes at the phylum level were especially affected. Correlation analysis further indicated a strong negative association between Bacteroidetes abundance and lipid metabolism−related parameters. Taken together, these data suggest that dietary HMB supplementation could improve abdominal fat deposition in layers, probably through modulating hepatic bile acid synthesis and gut microbiota function. |
format | Online Article Text |
id | pubmed-9406763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94067632022-08-26 Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens Liao, Qichao Wu, Tian Fu, Qinghua Wang, Peng Zhao, Yameng Li, Yan Xiao, Haihan Zhou, Lei Song, Ziyi Curr Issues Mol Biol Article Excessive lipid deposition in layer chickens due to inappropriate feeding adversely affects egg production; however, nutritional manipulation methods to deal with this issue are still limited. β-hydroxy-β-methylbutyrate (HMB), a metabolite of L-leucine, was recently reported as a lipid-lowering nutrient in mice and pigs, although its role in layers had not been investigated. Here, we employed high-fat and high-cholesterol diet (HFHCD)−challenged growing layers as an obese model to explore HMB function in the regulation of lipid metabolism and the potential mechanisms involved. We found that dietary supplementation with (0.05% or 0.10%) HMB significantly reduced HFHCD−induced bodyweight growth in layers, mainly due to reduction in abdominal fat deposition. Mechanistically, HMB supplementation enhanced hepatic bile acid synthesis from cholesterol through elevating expression of Cyp7a1, a gene coding a key enzyme in bile acid synthesis. Furthermore, 16S rRNA gene sequencing revealed that HMB supplementation remodeled the diversity and composition of the layers’ cecal microbiota, and the abundance of Bacteroidetes at the phylum level were especially affected. Correlation analysis further indicated a strong negative association between Bacteroidetes abundance and lipid metabolism−related parameters. Taken together, these data suggest that dietary HMB supplementation could improve abdominal fat deposition in layers, probably through modulating hepatic bile acid synthesis and gut microbiota function. MDPI 2022-07-30 /pmc/articles/PMC9406763/ /pubmed/36005131 http://dx.doi.org/10.3390/cimb44080235 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liao, Qichao Wu, Tian Fu, Qinghua Wang, Peng Zhao, Yameng Li, Yan Xiao, Haihan Zhou, Lei Song, Ziyi Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens |
title | Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens |
title_full | Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens |
title_fullStr | Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens |
title_full_unstemmed | Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens |
title_short | Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens |
title_sort | effects of dietary inclusion of β-hydroxy-β-methylbutyrate on growth performance, fat deposition, bile acid metabolism, and gut microbiota function in high-fat and high-cholesterol diet-challenged layer chickens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406763/ https://www.ncbi.nlm.nih.gov/pubmed/36005131 http://dx.doi.org/10.3390/cimb44080235 |
work_keys_str_mv | AT liaoqichao effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT wutian effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT fuqinghua effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT wangpeng effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT zhaoyameng effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT liyan effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT xiaohaihan effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT zhoulei effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens AT songziyi effectsofdietaryinclusionofbhydroxybmethylbutyrateongrowthperformancefatdepositionbileacidmetabolismandgutmicrobiotafunctioninhighfatandhighcholesteroldietchallengedlayerchickens |