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Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine

Idiopathic epiretinal membranes (iERMs) are fibrocellular sheets of tissue that develop at the vitreoretinal interface. The iERMs consist of cells and an extracellular matrix (ECM) formed by a complex array of structural proteins and a large number of proteins that regulate cell–matrix interaction,...

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Autores principales: Bianchi, Laura, Altera, Annalisa, Barone, Virginia, Bonente, Denise, Bacci, Tommaso, De Benedetto, Elena, Bini, Luca, Tosi, Gian Marco, Galvagni, Federico, Bertelli, Eugenio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406781/
https://www.ncbi.nlm.nih.gov/pubmed/36010606
http://dx.doi.org/10.3390/cells11162531
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author Bianchi, Laura
Altera, Annalisa
Barone, Virginia
Bonente, Denise
Bacci, Tommaso
De Benedetto, Elena
Bini, Luca
Tosi, Gian Marco
Galvagni, Federico
Bertelli, Eugenio
author_facet Bianchi, Laura
Altera, Annalisa
Barone, Virginia
Bonente, Denise
Bacci, Tommaso
De Benedetto, Elena
Bini, Luca
Tosi, Gian Marco
Galvagni, Federico
Bertelli, Eugenio
author_sort Bianchi, Laura
collection PubMed
description Idiopathic epiretinal membranes (iERMs) are fibrocellular sheets of tissue that develop at the vitreoretinal interface. The iERMs consist of cells and an extracellular matrix (ECM) formed by a complex array of structural proteins and a large number of proteins that regulate cell–matrix interaction, matrix deposition and remodelling. Many components of the ECM tend to produce a layered pattern that can influence the tractional properties of the membranes. We applied a bioinformatics approach on a list of proteins previously identified with an MS-based proteomic analysis on samples of iERM to report the interactome of some key proteins. The performed pathway analysis highlights interactions occurring among ECM molecules, their cell receptors and intra- or extracellular proteins that may play a role in matrix biology in this special context. In particular, integrin β1, cathepsin B, epidermal growth factor receptor, protein-glutamine gamma-glutamyltransferase 2 and prolow-density lipoprotein receptor-related protein 1 are key hubs in the outlined protein–protein cross-talks. A section on the biomarkers that can be found in the vitreous humor of patients affected by iERM and that can modulate matrix deposition is also presented. Finally, translational medicine in iERM treatment has been summed up taking stock of the techniques that have been proposed for pharmacologic vitreolysis.
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spelling pubmed-94067812022-08-26 Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine Bianchi, Laura Altera, Annalisa Barone, Virginia Bonente, Denise Bacci, Tommaso De Benedetto, Elena Bini, Luca Tosi, Gian Marco Galvagni, Federico Bertelli, Eugenio Cells Review Idiopathic epiretinal membranes (iERMs) are fibrocellular sheets of tissue that develop at the vitreoretinal interface. The iERMs consist of cells and an extracellular matrix (ECM) formed by a complex array of structural proteins and a large number of proteins that regulate cell–matrix interaction, matrix deposition and remodelling. Many components of the ECM tend to produce a layered pattern that can influence the tractional properties of the membranes. We applied a bioinformatics approach on a list of proteins previously identified with an MS-based proteomic analysis on samples of iERM to report the interactome of some key proteins. The performed pathway analysis highlights interactions occurring among ECM molecules, their cell receptors and intra- or extracellular proteins that may play a role in matrix biology in this special context. In particular, integrin β1, cathepsin B, epidermal growth factor receptor, protein-glutamine gamma-glutamyltransferase 2 and prolow-density lipoprotein receptor-related protein 1 are key hubs in the outlined protein–protein cross-talks. A section on the biomarkers that can be found in the vitreous humor of patients affected by iERM and that can modulate matrix deposition is also presented. Finally, translational medicine in iERM treatment has been summed up taking stock of the techniques that have been proposed for pharmacologic vitreolysis. MDPI 2022-08-15 /pmc/articles/PMC9406781/ /pubmed/36010606 http://dx.doi.org/10.3390/cells11162531 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Bianchi, Laura
Altera, Annalisa
Barone, Virginia
Bonente, Denise
Bacci, Tommaso
De Benedetto, Elena
Bini, Luca
Tosi, Gian Marco
Galvagni, Federico
Bertelli, Eugenio
Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine
title Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine
title_full Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine
title_fullStr Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine
title_full_unstemmed Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine
title_short Untangling the Extracellular Matrix of Idiopathic Epiretinal Membrane: A Path Winding among Structure, Interactomics and Translational Medicine
title_sort untangling the extracellular matrix of idiopathic epiretinal membrane: a path winding among structure, interactomics and translational medicine
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406781/
https://www.ncbi.nlm.nih.gov/pubmed/36010606
http://dx.doi.org/10.3390/cells11162531
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