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Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase

The advantages of cryogels for enzyme immobilization applications include their mechanical and chemical robustness, ease of production, superior porosity, and low cost. Currently, many researchers are exploring porous material-based systems for enzyme immobilization that are more efficient and econo...

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Autores principales: Altunbaş, Canan, Aslan, Ahmet, Kuşat, Kevser, Sahiner, Mehtap, Akgöl, Sinan, Sahiner, Nurettin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407055/
https://www.ncbi.nlm.nih.gov/pubmed/36005102
http://dx.doi.org/10.3390/gels8080501
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author Altunbaş, Canan
Aslan, Ahmet
Kuşat, Kevser
Sahiner, Mehtap
Akgöl, Sinan
Sahiner, Nurettin
author_facet Altunbaş, Canan
Aslan, Ahmet
Kuşat, Kevser
Sahiner, Mehtap
Akgöl, Sinan
Sahiner, Nurettin
author_sort Altunbaş, Canan
collection PubMed
description The advantages of cryogels for enzyme immobilization applications include their mechanical and chemical robustness, ease of production, superior porosity, and low cost. Currently, many researchers are exploring porous material-based systems for enzyme immobilization that are more efficient and economically viable. Here, poly(2-Hydroxyethyl methacrylate-co-allyl glycidyl ether) (p(HEMA-co-AGE)) cryogel matrices were synthesized via the free radical cryopolymerization method to be employed as the support material. For the immobilization of the catalase enzyme onto the p(HEMA-co-AGE) cryogel matrix (catalase@p(HEMA-co-AGE), the best possible reaction conditions were determined by altering parameters such as pH, catalase initial concentration, and flow rate. The maximum catalase immobilization amount onto the p(HEMA-co-AGE) cryogel was found to be 48 mg/g cryogel. To determine the advantages of the cryogel matrix, e.g., the stability and reusability of the cryogel matrix, the adsorption–desorption cycles for the catalase enzyme were repeated five times using the same cryogel matrix. At the end of the reusability tests, it was found that the cryogel was very stable and maintained its adsorption capacity with the recovery ratio of 93.8 ± 1.2%. Therefore, the p(HEMA-co-AGE) cryogel matrix affords repeated useability, e.g., up to five times, without decreasing its catalase binding capacities significantly and has promising potential for many industrial applications. Cryogels offer clear distinctive advantages over common materials, e.g., micro/nano particles, hydrogels, films, and composites for these applications. At present, many researchers are working on the design of more effective and economically feasible, porous material-based systems for enzyme immobilization
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spelling pubmed-94070552022-08-26 Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase Altunbaş, Canan Aslan, Ahmet Kuşat, Kevser Sahiner, Mehtap Akgöl, Sinan Sahiner, Nurettin Gels Article The advantages of cryogels for enzyme immobilization applications include their mechanical and chemical robustness, ease of production, superior porosity, and low cost. Currently, many researchers are exploring porous material-based systems for enzyme immobilization that are more efficient and economically viable. Here, poly(2-Hydroxyethyl methacrylate-co-allyl glycidyl ether) (p(HEMA-co-AGE)) cryogel matrices were synthesized via the free radical cryopolymerization method to be employed as the support material. For the immobilization of the catalase enzyme onto the p(HEMA-co-AGE) cryogel matrix (catalase@p(HEMA-co-AGE), the best possible reaction conditions were determined by altering parameters such as pH, catalase initial concentration, and flow rate. The maximum catalase immobilization amount onto the p(HEMA-co-AGE) cryogel was found to be 48 mg/g cryogel. To determine the advantages of the cryogel matrix, e.g., the stability and reusability of the cryogel matrix, the adsorption–desorption cycles for the catalase enzyme were repeated five times using the same cryogel matrix. At the end of the reusability tests, it was found that the cryogel was very stable and maintained its adsorption capacity with the recovery ratio of 93.8 ± 1.2%. Therefore, the p(HEMA-co-AGE) cryogel matrix affords repeated useability, e.g., up to five times, without decreasing its catalase binding capacities significantly and has promising potential for many industrial applications. Cryogels offer clear distinctive advantages over common materials, e.g., micro/nano particles, hydrogels, films, and composites for these applications. At present, many researchers are working on the design of more effective and economically feasible, porous material-based systems for enzyme immobilization MDPI 2022-08-12 /pmc/articles/PMC9407055/ /pubmed/36005102 http://dx.doi.org/10.3390/gels8080501 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Altunbaş, Canan
Aslan, Ahmet
Kuşat, Kevser
Sahiner, Mehtap
Akgöl, Sinan
Sahiner, Nurettin
Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
title Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
title_full Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
title_fullStr Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
title_full_unstemmed Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
title_short Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
title_sort synthesis and characterization of a new cryogel matrix for covalent immobilization of catalase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407055/
https://www.ncbi.nlm.nih.gov/pubmed/36005102
http://dx.doi.org/10.3390/gels8080501
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