Cargando…
Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407060/ https://www.ncbi.nlm.nih.gov/pubmed/36010314 http://dx.doi.org/10.3390/diagnostics12081964 |
_version_ | 1784774272202309632 |
---|---|
author | Liu, Juan-Juan Teng, Li-Ping Hua, Chun-Zhen Xie, Yong-Ping Pan, Yan-Xiang Hu, Bo-Fei Hu, Wei-Lin Wang, Wei-Jian |
author_facet | Liu, Juan-Juan Teng, Li-Ping Hua, Chun-Zhen Xie, Yong-Ping Pan, Yan-Xiang Hu, Bo-Fei Hu, Wei-Lin Wang, Wei-Jian |
author_sort | Liu, Juan-Juan |
collection | PubMed |
description | Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province from January 2018 to December 2019. The samples were tested using multiplex and singleplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with primers specific to enterovirus (EV), varicella-zoster virus (VZV), mumps virus (MuV), cytomegalovirus (CMV), herpes simplex virus type 1 (HSV-1)/type 2 (HSV-2), Epstein–Barr virus (EBV), and human herpesvirus 6 (HHV-6). The data of the two analyses were compared and then verified using Sanger sequencing. Results: Of the 227 CSF samples, 90 were shown to be positive for multiplex RT-PCR with a positivity rate of 39.65% and a 95% confidence interval (33.2%, 46.1%). EV was the most common cause of VE, followed by EBV, HHV-6, MuV, CMV, VZV, and HSV-1. Most included cases occurred in summer, accounting for 49.78% of all cases. For EV, EBV, and HSV-2, multiplex RT-PCR showed a positivity rate of 34.36%, which was not statistically different from that of 30.4% shown by singleplex RT-PCR. The sequences of EV, EBV, VZV, MuV, CMV, HSV-1, HHV-6, and HSV-2 were confirmed by sequencing the PCR products obtained from multiplex and singleplex PCR. Conclusions: In children, VE is more prevalent in the summer than in other seasons in Zhejiang province, and EV may be the most common causative pathogen. |
format | Online Article Text |
id | pubmed-9407060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94070602022-08-26 Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 Liu, Juan-Juan Teng, Li-Ping Hua, Chun-Zhen Xie, Yong-Ping Pan, Yan-Xiang Hu, Bo-Fei Hu, Wei-Lin Wang, Wei-Jian Diagnostics (Basel) Article Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province from January 2018 to December 2019. The samples were tested using multiplex and singleplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with primers specific to enterovirus (EV), varicella-zoster virus (VZV), mumps virus (MuV), cytomegalovirus (CMV), herpes simplex virus type 1 (HSV-1)/type 2 (HSV-2), Epstein–Barr virus (EBV), and human herpesvirus 6 (HHV-6). The data of the two analyses were compared and then verified using Sanger sequencing. Results: Of the 227 CSF samples, 90 were shown to be positive for multiplex RT-PCR with a positivity rate of 39.65% and a 95% confidence interval (33.2%, 46.1%). EV was the most common cause of VE, followed by EBV, HHV-6, MuV, CMV, VZV, and HSV-1. Most included cases occurred in summer, accounting for 49.78% of all cases. For EV, EBV, and HSV-2, multiplex RT-PCR showed a positivity rate of 34.36%, which was not statistically different from that of 30.4% shown by singleplex RT-PCR. The sequences of EV, EBV, VZV, MuV, CMV, HSV-1, HHV-6, and HSV-2 were confirmed by sequencing the PCR products obtained from multiplex and singleplex PCR. Conclusions: In children, VE is more prevalent in the summer than in other seasons in Zhejiang province, and EV may be the most common causative pathogen. MDPI 2022-08-14 /pmc/articles/PMC9407060/ /pubmed/36010314 http://dx.doi.org/10.3390/diagnostics12081964 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Juan-Juan Teng, Li-Ping Hua, Chun-Zhen Xie, Yong-Ping Pan, Yan-Xiang Hu, Bo-Fei Hu, Wei-Lin Wang, Wei-Jian Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 |
title | Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 |
title_full | Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 |
title_fullStr | Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 |
title_full_unstemmed | Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 |
title_short | Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 |
title_sort | etiological analysis of viral encephalitis in children in zhejiang province from 2018 to 2019 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407060/ https://www.ncbi.nlm.nih.gov/pubmed/36010314 http://dx.doi.org/10.3390/diagnostics12081964 |
work_keys_str_mv | AT liujuanjuan etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT tengliping etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT huachunzhen etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT xieyongping etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT panyanxiang etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT hubofei etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT huweilin etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 AT wangweijian etiologicalanalysisofviralencephalitisinchildreninzhejiangprovincefrom2018to2019 |