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Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019

Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province...

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Autores principales: Liu, Juan-Juan, Teng, Li-Ping, Hua, Chun-Zhen, Xie, Yong-Ping, Pan, Yan-Xiang, Hu, Bo-Fei, Hu, Wei-Lin, Wang, Wei-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407060/
https://www.ncbi.nlm.nih.gov/pubmed/36010314
http://dx.doi.org/10.3390/diagnostics12081964
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author Liu, Juan-Juan
Teng, Li-Ping
Hua, Chun-Zhen
Xie, Yong-Ping
Pan, Yan-Xiang
Hu, Bo-Fei
Hu, Wei-Lin
Wang, Wei-Jian
author_facet Liu, Juan-Juan
Teng, Li-Ping
Hua, Chun-Zhen
Xie, Yong-Ping
Pan, Yan-Xiang
Hu, Bo-Fei
Hu, Wei-Lin
Wang, Wei-Jian
author_sort Liu, Juan-Juan
collection PubMed
description Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province from January 2018 to December 2019. The samples were tested using multiplex and singleplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with primers specific to enterovirus (EV), varicella-zoster virus (VZV), mumps virus (MuV), cytomegalovirus (CMV), herpes simplex virus type 1 (HSV-1)/type 2 (HSV-2), Epstein–Barr virus (EBV), and human herpesvirus 6 (HHV-6). The data of the two analyses were compared and then verified using Sanger sequencing. Results: Of the 227 CSF samples, 90 were shown to be positive for multiplex RT-PCR with a positivity rate of 39.65% and a 95% confidence interval (33.2%, 46.1%). EV was the most common cause of VE, followed by EBV, HHV-6, MuV, CMV, VZV, and HSV-1. Most included cases occurred in summer, accounting for 49.78% of all cases. For EV, EBV, and HSV-2, multiplex RT-PCR showed a positivity rate of 34.36%, which was not statistically different from that of 30.4% shown by singleplex RT-PCR. The sequences of EV, EBV, VZV, MuV, CMV, HSV-1, HHV-6, and HSV-2 were confirmed by sequencing the PCR products obtained from multiplex and singleplex PCR. Conclusions: In children, VE is more prevalent in the summer than in other seasons in Zhejiang province, and EV may be the most common causative pathogen.
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spelling pubmed-94070602022-08-26 Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019 Liu, Juan-Juan Teng, Li-Ping Hua, Chun-Zhen Xie, Yong-Ping Pan, Yan-Xiang Hu, Bo-Fei Hu, Wei-Lin Wang, Wei-Jian Diagnostics (Basel) Article Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province from January 2018 to December 2019. The samples were tested using multiplex and singleplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with primers specific to enterovirus (EV), varicella-zoster virus (VZV), mumps virus (MuV), cytomegalovirus (CMV), herpes simplex virus type 1 (HSV-1)/type 2 (HSV-2), Epstein–Barr virus (EBV), and human herpesvirus 6 (HHV-6). The data of the two analyses were compared and then verified using Sanger sequencing. Results: Of the 227 CSF samples, 90 were shown to be positive for multiplex RT-PCR with a positivity rate of 39.65% and a 95% confidence interval (33.2%, 46.1%). EV was the most common cause of VE, followed by EBV, HHV-6, MuV, CMV, VZV, and HSV-1. Most included cases occurred in summer, accounting for 49.78% of all cases. For EV, EBV, and HSV-2, multiplex RT-PCR showed a positivity rate of 34.36%, which was not statistically different from that of 30.4% shown by singleplex RT-PCR. The sequences of EV, EBV, VZV, MuV, CMV, HSV-1, HHV-6, and HSV-2 were confirmed by sequencing the PCR products obtained from multiplex and singleplex PCR. Conclusions: In children, VE is more prevalent in the summer than in other seasons in Zhejiang province, and EV may be the most common causative pathogen. MDPI 2022-08-14 /pmc/articles/PMC9407060/ /pubmed/36010314 http://dx.doi.org/10.3390/diagnostics12081964 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Juan-Juan
Teng, Li-Ping
Hua, Chun-Zhen
Xie, Yong-Ping
Pan, Yan-Xiang
Hu, Bo-Fei
Hu, Wei-Lin
Wang, Wei-Jian
Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
title Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
title_full Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
title_fullStr Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
title_full_unstemmed Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
title_short Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019
title_sort etiological analysis of viral encephalitis in children in zhejiang province from 2018 to 2019
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407060/
https://www.ncbi.nlm.nih.gov/pubmed/36010314
http://dx.doi.org/10.3390/diagnostics12081964
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