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Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity
BACKGROUND: Little is known about the impact of mango consumption on metabolic pathways assessed by changes in gene expression. METHODS: In this single-arm clinical trial, cardiometabolic outcomes and gene expression levels in whole blood samples from 26 men and women were examined at baseline and a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407242/ https://www.ncbi.nlm.nih.gov/pubmed/36034894 http://dx.doi.org/10.3389/fnut.2022.918844 |
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author | Keathley, Justine de Toro-Martín, Juan Kearney, Michèle Garneau, Véronique Pilon, Geneviève Couture, Patrick Marette, André Vohl, Marie-Claude Couillard, Charles |
author_facet | Keathley, Justine de Toro-Martín, Juan Kearney, Michèle Garneau, Véronique Pilon, Geneviève Couture, Patrick Marette, André Vohl, Marie-Claude Couillard, Charles |
author_sort | Keathley, Justine |
collection | PubMed |
description | BACKGROUND: Little is known about the impact of mango consumption on metabolic pathways assessed by changes in gene expression. METHODS: In this single-arm clinical trial, cardiometabolic outcomes and gene expression levels in whole blood samples from 26 men and women were examined at baseline and after 8 weeks of mango consumption and differential gene expression changes were determined. Based on changes in gene expression profiles, partial least squares discriminant analysis followed by hierarchical clustering were used to classify participants into subgroups of response and differences in gene expression changes and in cardiometabolic clinical outcomes following the intervention were tested. RESULTS: Two subgroups of participants were separated based on the resemblance of gene expression profiles in response to the intervention and as responders (n = 8) and non-responders (n = 18). A total of 280 transcripts were significantly up-regulated and 603 transcripts down-regulated following the intervention in responders, as compared to non-responders. Several metabolic pathways, mainly related to oxygen and carbon dioxide transport as well as oxidative stress, were found to be significantly enriched with differentially expressed genes. In addition, significantly beneficial changes in hip and waist circumference, c-reactive protein, HOMA-IR and QUICKI indices were observed in responders vs. non-responders, following the intervention. CONCLUSION: The impact of mango consumption on cardiometabolic health appears to largely rely on interindividual variability. The novel transcriptomic-based clustering analysis used herein can provide insights for future research focused on unveiling the origins of heterogeneous responses to dietary interventions. CLINICAL TRIAL REGISTRATION: [clinicaltrials.gov], identifier [NCT03825276]. |
format | Online Article Text |
id | pubmed-9407242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94072422022-08-26 Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity Keathley, Justine de Toro-Martín, Juan Kearney, Michèle Garneau, Véronique Pilon, Geneviève Couture, Patrick Marette, André Vohl, Marie-Claude Couillard, Charles Front Nutr Nutrition BACKGROUND: Little is known about the impact of mango consumption on metabolic pathways assessed by changes in gene expression. METHODS: In this single-arm clinical trial, cardiometabolic outcomes and gene expression levels in whole blood samples from 26 men and women were examined at baseline and after 8 weeks of mango consumption and differential gene expression changes were determined. Based on changes in gene expression profiles, partial least squares discriminant analysis followed by hierarchical clustering were used to classify participants into subgroups of response and differences in gene expression changes and in cardiometabolic clinical outcomes following the intervention were tested. RESULTS: Two subgroups of participants were separated based on the resemblance of gene expression profiles in response to the intervention and as responders (n = 8) and non-responders (n = 18). A total of 280 transcripts were significantly up-regulated and 603 transcripts down-regulated following the intervention in responders, as compared to non-responders. Several metabolic pathways, mainly related to oxygen and carbon dioxide transport as well as oxidative stress, were found to be significantly enriched with differentially expressed genes. In addition, significantly beneficial changes in hip and waist circumference, c-reactive protein, HOMA-IR and QUICKI indices were observed in responders vs. non-responders, following the intervention. CONCLUSION: The impact of mango consumption on cardiometabolic health appears to largely rely on interindividual variability. The novel transcriptomic-based clustering analysis used herein can provide insights for future research focused on unveiling the origins of heterogeneous responses to dietary interventions. CLINICAL TRIAL REGISTRATION: [clinicaltrials.gov], identifier [NCT03825276]. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9407242/ /pubmed/36034894 http://dx.doi.org/10.3389/fnut.2022.918844 Text en Copyright © 2022 Keathley, de Toro-Martín, Kearney, Garneau, Pilon, Couture, Marette, Vohl and Couillard. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Keathley, Justine de Toro-Martín, Juan Kearney, Michèle Garneau, Véronique Pilon, Geneviève Couture, Patrick Marette, André Vohl, Marie-Claude Couillard, Charles Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
title | Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
title_full | Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
title_fullStr | Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
title_full_unstemmed | Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
title_short | Gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
title_sort | gene expression signatures and cardiometabolic outcomes following 8-week mango consumption in individuals with overweight/obesity |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407242/ https://www.ncbi.nlm.nih.gov/pubmed/36034894 http://dx.doi.org/10.3389/fnut.2022.918844 |
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