Cargando…

Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation

High-Pressure Processing’s (HPP) non-thermal inactivation of cells has been largely incompatible with food products in which the activity of selected cultures is intended (e.g., bio-preservation). This work aims to overcome this limitation using a cocoa butter encapsulation system for freeze-dried c...

Descripción completa

Detalles Bibliográficos
Autores principales: McGillin, Meghan R., deRiancho, Dana L., DeMarsh, Timothy A., Hsu, Ella D., Alcaine, Samuel D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407356/
https://www.ncbi.nlm.nih.gov/pubmed/36010466
http://dx.doi.org/10.3390/foods11162465
_version_ 1784774344333852672
author McGillin, Meghan R.
deRiancho, Dana L.
DeMarsh, Timothy A.
Hsu, Ella D.
Alcaine, Samuel D.
author_facet McGillin, Meghan R.
deRiancho, Dana L.
DeMarsh, Timothy A.
Hsu, Ella D.
Alcaine, Samuel D.
author_sort McGillin, Meghan R.
collection PubMed
description High-Pressure Processing’s (HPP) non-thermal inactivation of cells has been largely incompatible with food products in which the activity of selected cultures is intended (e.g., bio-preservation). This work aims to overcome this limitation using a cocoa butter encapsulation system for freeze-dried cultures that can be integrated with HPP technology with minimal detrimental effects on cell viability or activity capabilities. Using commercially available freeze-dried protective cultures, the desiccated cells survived HPP (600 MPa, 5 °C, 3 min) and subsequently experienced a 0.66-log increase in cell counts during 2 h of incubation. When the same culture was rehydrated prior to HPP, it underwent a >6.07-log decrease. Phosphate-buffered saline or skim milk inoculated with cocoa butter-encapsulated culture up to 24 h before HPP displayed robust cell counts after HPP and subsequent plating (8.37–9.16 CFU/mL). In addition to assessing viability following HPP, the study sought to test the applicability in a product in which post-HPP fermentation is desired While HPP-treated encapsulated cultures initially exhibited significantly delayed fermentative processes compared to the positive controls, by 48 h following inoculation, the HPP samples’ pH values bore no significant difference from those of the positive controls (encapsulated samples: pH 3.83 to 3.92; positive controls: pH 3.81 to 3.85). The HPP encapsulated cultures also maintained high cell counts throughout the fermentation (≥8.95 log CFU/mL).
format Online
Article
Text
id pubmed-9407356
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94073562022-08-26 Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation McGillin, Meghan R. deRiancho, Dana L. DeMarsh, Timothy A. Hsu, Ella D. Alcaine, Samuel D. Foods Article High-Pressure Processing’s (HPP) non-thermal inactivation of cells has been largely incompatible with food products in which the activity of selected cultures is intended (e.g., bio-preservation). This work aims to overcome this limitation using a cocoa butter encapsulation system for freeze-dried cultures that can be integrated with HPP technology with minimal detrimental effects on cell viability or activity capabilities. Using commercially available freeze-dried protective cultures, the desiccated cells survived HPP (600 MPa, 5 °C, 3 min) and subsequently experienced a 0.66-log increase in cell counts during 2 h of incubation. When the same culture was rehydrated prior to HPP, it underwent a >6.07-log decrease. Phosphate-buffered saline or skim milk inoculated with cocoa butter-encapsulated culture up to 24 h before HPP displayed robust cell counts after HPP and subsequent plating (8.37–9.16 CFU/mL). In addition to assessing viability following HPP, the study sought to test the applicability in a product in which post-HPP fermentation is desired While HPP-treated encapsulated cultures initially exhibited significantly delayed fermentative processes compared to the positive controls, by 48 h following inoculation, the HPP samples’ pH values bore no significant difference from those of the positive controls (encapsulated samples: pH 3.83 to 3.92; positive controls: pH 3.81 to 3.85). The HPP encapsulated cultures also maintained high cell counts throughout the fermentation (≥8.95 log CFU/mL). MDPI 2022-08-16 /pmc/articles/PMC9407356/ /pubmed/36010466 http://dx.doi.org/10.3390/foods11162465 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
McGillin, Meghan R.
deRiancho, Dana L.
DeMarsh, Timothy A.
Hsu, Ella D.
Alcaine, Samuel D.
Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation
title Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation
title_full Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation
title_fullStr Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation
title_full_unstemmed Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation
title_short Selective Survival of Protective Cultures during High-Pressure Processing by Leveraging Freeze-Drying and Encapsulation
title_sort selective survival of protective cultures during high-pressure processing by leveraging freeze-drying and encapsulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407356/
https://www.ncbi.nlm.nih.gov/pubmed/36010466
http://dx.doi.org/10.3390/foods11162465
work_keys_str_mv AT mcgillinmeghanr selectivesurvivalofprotectiveculturesduringhighpressureprocessingbyleveragingfreezedryingandencapsulation
AT derianchodanal selectivesurvivalofprotectiveculturesduringhighpressureprocessingbyleveragingfreezedryingandencapsulation
AT demarshtimothya selectivesurvivalofprotectiveculturesduringhighpressureprocessingbyleveragingfreezedryingandencapsulation
AT hsuellad selectivesurvivalofprotectiveculturesduringhighpressureprocessingbyleveragingfreezedryingandencapsulation
AT alcainesamueld selectivesurvivalofprotectiveculturesduringhighpressureprocessingbyleveragingfreezedryingandencapsulation