Cargando…

Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10

Obesity has become a major social problem related to health and quality of life. Our previous work demonstrated that Lactobacillus plantarum FRT10 alleviated obesity in high-fat diet (HFD)-fed mice by alleviating gut dysbiosis. However, the underlying functions of FRT10 in regulating liver and cecum...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Hongying, Li, Daojie, Song, Liye, Xu, Xin, Han, Yunsheng, Meng, Kun, Wen, Zhiguo, Yang, Peilong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407591/
https://www.ncbi.nlm.nih.gov/pubmed/36010491
http://dx.doi.org/10.3390/foods11162491
_version_ 1784774401467613184
author Cai, Hongying
Li, Daojie
Song, Liye
Xu, Xin
Han, Yunsheng
Meng, Kun
Wen, Zhiguo
Yang, Peilong
author_facet Cai, Hongying
Li, Daojie
Song, Liye
Xu, Xin
Han, Yunsheng
Meng, Kun
Wen, Zhiguo
Yang, Peilong
author_sort Cai, Hongying
collection PubMed
description Obesity has become a major social problem related to health and quality of life. Our previous work demonstrated that Lactobacillus plantarum FRT10 alleviated obesity in high-fat diet (HFD)-fed mice by alleviating gut dysbiosis. However, the underlying functions of FRT10 in regulating liver and cecum contents metabolism remain unknown. Liver and cecum contents metabonomics combined with pathway analysis based on ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) were performed to evaluate the alterations of metabolic profiles between obese control mice and obese mice in FRT10-treated groups. The orthogonal partial least squares discriminant analysis (OPLS-DA) score plots showed that there were significant differences in cecum contents and liver markers between experimental groups. In total, 26 potential biomarkers were identified in the liver and 15 in cecum contents that could explain the effect of FRT10 addition in HFD-fed mice. In addition, gut–liver axis analysis indicated that there was a strong correlation between cecum contents metabolites and hepatic metabolites. The mechanism of FRT10 against obesity might be related to the alterations in glycerophospholipid metabolism, primary bile acid biosynthesis, amino metabolism, and purine and pyrimidine metabolism. Studies on these metabolites could help us better understand the role of FRT10 in obesity induced by HFD.
format Online
Article
Text
id pubmed-9407591
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94075912022-08-26 Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10 Cai, Hongying Li, Daojie Song, Liye Xu, Xin Han, Yunsheng Meng, Kun Wen, Zhiguo Yang, Peilong Foods Article Obesity has become a major social problem related to health and quality of life. Our previous work demonstrated that Lactobacillus plantarum FRT10 alleviated obesity in high-fat diet (HFD)-fed mice by alleviating gut dysbiosis. However, the underlying functions of FRT10 in regulating liver and cecum contents metabolism remain unknown. Liver and cecum contents metabonomics combined with pathway analysis based on ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) were performed to evaluate the alterations of metabolic profiles between obese control mice and obese mice in FRT10-treated groups. The orthogonal partial least squares discriminant analysis (OPLS-DA) score plots showed that there were significant differences in cecum contents and liver markers between experimental groups. In total, 26 potential biomarkers were identified in the liver and 15 in cecum contents that could explain the effect of FRT10 addition in HFD-fed mice. In addition, gut–liver axis analysis indicated that there was a strong correlation between cecum contents metabolites and hepatic metabolites. The mechanism of FRT10 against obesity might be related to the alterations in glycerophospholipid metabolism, primary bile acid biosynthesis, amino metabolism, and purine and pyrimidine metabolism. Studies on these metabolites could help us better understand the role of FRT10 in obesity induced by HFD. MDPI 2022-08-18 /pmc/articles/PMC9407591/ /pubmed/36010491 http://dx.doi.org/10.3390/foods11162491 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cai, Hongying
Li, Daojie
Song, Liye
Xu, Xin
Han, Yunsheng
Meng, Kun
Wen, Zhiguo
Yang, Peilong
Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10
title Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10
title_full Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10
title_fullStr Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10
title_full_unstemmed Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10
title_short Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10
title_sort metabolomic characteristics of liver and cecum contents in high-fat-diet-induced obese mice intervened with lactobacillus plantarum frt10
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407591/
https://www.ncbi.nlm.nih.gov/pubmed/36010491
http://dx.doi.org/10.3390/foods11162491
work_keys_str_mv AT caihongying metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT lidaojie metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT songliye metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT xuxin metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT hanyunsheng metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT mengkun metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT wenzhiguo metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10
AT yangpeilong metabolomiccharacteristicsofliverandcecumcontentsinhighfatdietinducedobesemiceintervenedwithlactobacillusplantarumfrt10