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On the formation of ordered protein assemblies in cell–cell interfaces
Crystal structures of many cell–cell adhesion receptors reveal the formation of linear “molecular zippers” comprising an ordered one-dimensional array of proteins that form both intercellular (trans) and intracellular (cis) interactions. The clustered protocadherins (cPcdhs) provide an exemplar of t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407605/ https://www.ncbi.nlm.nih.gov/pubmed/35969779 http://dx.doi.org/10.1073/pnas.2206175119 |
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author | Boni, Nadir Shapiro, Lawrence Honig, Barry Wu, Yinghao Rubinstein, Rotem |
author_facet | Boni, Nadir Shapiro, Lawrence Honig, Barry Wu, Yinghao Rubinstein, Rotem |
author_sort | Boni, Nadir |
collection | PubMed |
description | Crystal structures of many cell–cell adhesion receptors reveal the formation of linear “molecular zippers” comprising an ordered one-dimensional array of proteins that form both intercellular (trans) and intracellular (cis) interactions. The clustered protocadherins (cPcdhs) provide an exemplar of this phenomenon and use it as a basis of barcoding of vertebrate neurons. Here, we report both Metropolis and kinetic Monte Carlo simulations of cPcdh zipper formation using simplified models of cPcdhs that nevertheless capture essential features of their three-dimensional structure. The simulations reveal that the formation of long zippers is an implicit feature of cPcdh structure and is driven by their cis and trans interactions that have been quantitatively characterized in previous work. Moreover, in agreement with cryo-electron tomography studies, the zippers are found to organize into two-dimensional arrays even in the absence of attractive interactions between individual zippers. Our results suggest that the formation of ordered two-dimensional arrays of linear zippers of adhesion proteins is a common feature of cell–cell interfaces. From the perspective of simulations, they demonstrate the importance of a realistic depiction of adhesion protein structure and interactions if important biological phenomena are to be properly captured. |
format | Online Article Text |
id | pubmed-9407605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-94076052023-02-15 On the formation of ordered protein assemblies in cell–cell interfaces Boni, Nadir Shapiro, Lawrence Honig, Barry Wu, Yinghao Rubinstein, Rotem Proc Natl Acad Sci U S A Biological Sciences Crystal structures of many cell–cell adhesion receptors reveal the formation of linear “molecular zippers” comprising an ordered one-dimensional array of proteins that form both intercellular (trans) and intracellular (cis) interactions. The clustered protocadherins (cPcdhs) provide an exemplar of this phenomenon and use it as a basis of barcoding of vertebrate neurons. Here, we report both Metropolis and kinetic Monte Carlo simulations of cPcdh zipper formation using simplified models of cPcdhs that nevertheless capture essential features of their three-dimensional structure. The simulations reveal that the formation of long zippers is an implicit feature of cPcdh structure and is driven by their cis and trans interactions that have been quantitatively characterized in previous work. Moreover, in agreement with cryo-electron tomography studies, the zippers are found to organize into two-dimensional arrays even in the absence of attractive interactions between individual zippers. Our results suggest that the formation of ordered two-dimensional arrays of linear zippers of adhesion proteins is a common feature of cell–cell interfaces. From the perspective of simulations, they demonstrate the importance of a realistic depiction of adhesion protein structure and interactions if important biological phenomena are to be properly captured. National Academy of Sciences 2022-08-15 2022-08-23 /pmc/articles/PMC9407605/ /pubmed/35969779 http://dx.doi.org/10.1073/pnas.2206175119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Boni, Nadir Shapiro, Lawrence Honig, Barry Wu, Yinghao Rubinstein, Rotem On the formation of ordered protein assemblies in cell–cell interfaces |
title | On the formation of ordered protein assemblies in cell–cell interfaces |
title_full | On the formation of ordered protein assemblies in cell–cell interfaces |
title_fullStr | On the formation of ordered protein assemblies in cell–cell interfaces |
title_full_unstemmed | On the formation of ordered protein assemblies in cell–cell interfaces |
title_short | On the formation of ordered protein assemblies in cell–cell interfaces |
title_sort | on the formation of ordered protein assemblies in cell–cell interfaces |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407605/ https://www.ncbi.nlm.nih.gov/pubmed/35969779 http://dx.doi.org/10.1073/pnas.2206175119 |
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