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Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM
We employed in a correlative manner an unconventional combination of methods, comprising cathodoluminescence, cryo–scanning electron microscopy (SEM), and cryo–focused ion beam (FIB)-SEM, to examine the volumes of thousands of cubed micrometers from rabbit atherosclerotic tissues, maintained in clos...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407640/ https://www.ncbi.nlm.nih.gov/pubmed/35939716 http://dx.doi.org/10.1073/pnas.2205475119 |
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author | Capua-Shenkar, Jenny Varsano, Neta Itzhak, Noya-Ruth Kaplan-Ashiri, Ifat Rechav, Katya Jin, Xueting Niimi, Manabu Fan, Jianglin Kruth, Howard S. Addadi, Lia |
author_facet | Capua-Shenkar, Jenny Varsano, Neta Itzhak, Noya-Ruth Kaplan-Ashiri, Ifat Rechav, Katya Jin, Xueting Niimi, Manabu Fan, Jianglin Kruth, Howard S. Addadi, Lia |
author_sort | Capua-Shenkar, Jenny |
collection | PubMed |
description | We employed in a correlative manner an unconventional combination of methods, comprising cathodoluminescence, cryo–scanning electron microscopy (SEM), and cryo–focused ion beam (FIB)-SEM, to examine the volumes of thousands of cubed micrometers from rabbit atherosclerotic tissues, maintained in close-to-native conditions, with a resolution of tens of nanometers. Data from three different intralesional regions, at the media–lesion interface, in the core, and toward the lumen, were analyzed following segmentation and volume or surface representation. The media–lesion interface region is rich in cells and lipid droplets, whereas the core region is markedly richer in crystals and has lower cell density. In the three regions, thin crystals appear to be associated with intracellular or extracellular lipid droplets and multilamellar bodies. Large crystals are independently positioned in the tissue, not associated with specific cellular components. This extensive evidence strongly supports the idea that the lipid droplet surfaces and the outer membranes of multilamellar bodies play a role in cholesterol crystal nucleation and growth and that crystal formation occurs, in part, inside cells. The correlative combination of methods that allowed the direct examination of cholesterol crystals and lipid deposits in the atherosclerotic lesions may be similarly used for high-resolution examination of other tissues containing pathological or physiological cholesterol deposits. |
format | Online Article Text |
id | pubmed-9407640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-94076402023-02-08 Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM Capua-Shenkar, Jenny Varsano, Neta Itzhak, Noya-Ruth Kaplan-Ashiri, Ifat Rechav, Katya Jin, Xueting Niimi, Manabu Fan, Jianglin Kruth, Howard S. Addadi, Lia Proc Natl Acad Sci U S A Physical Sciences We employed in a correlative manner an unconventional combination of methods, comprising cathodoluminescence, cryo–scanning electron microscopy (SEM), and cryo–focused ion beam (FIB)-SEM, to examine the volumes of thousands of cubed micrometers from rabbit atherosclerotic tissues, maintained in close-to-native conditions, with a resolution of tens of nanometers. Data from three different intralesional regions, at the media–lesion interface, in the core, and toward the lumen, were analyzed following segmentation and volume or surface representation. The media–lesion interface region is rich in cells and lipid droplets, whereas the core region is markedly richer in crystals and has lower cell density. In the three regions, thin crystals appear to be associated with intracellular or extracellular lipid droplets and multilamellar bodies. Large crystals are independently positioned in the tissue, not associated with specific cellular components. This extensive evidence strongly supports the idea that the lipid droplet surfaces and the outer membranes of multilamellar bodies play a role in cholesterol crystal nucleation and growth and that crystal formation occurs, in part, inside cells. The correlative combination of methods that allowed the direct examination of cholesterol crystals and lipid deposits in the atherosclerotic lesions may be similarly used for high-resolution examination of other tissues containing pathological or physiological cholesterol deposits. National Academy of Sciences 2022-08-08 2022-08-23 /pmc/articles/PMC9407640/ /pubmed/35939716 http://dx.doi.org/10.1073/pnas.2205475119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Physical Sciences Capua-Shenkar, Jenny Varsano, Neta Itzhak, Noya-Ruth Kaplan-Ashiri, Ifat Rechav, Katya Jin, Xueting Niimi, Manabu Fan, Jianglin Kruth, Howard S. Addadi, Lia Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM |
title | Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM |
title_full | Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM |
title_fullStr | Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM |
title_full_unstemmed | Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM |
title_short | Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM |
title_sort | examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-fib-sem |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407640/ https://www.ncbi.nlm.nih.gov/pubmed/35939716 http://dx.doi.org/10.1073/pnas.2205475119 |
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