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Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues

Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of ma...

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Autores principales: Kobayashi, Mako, Ishida, Naoki, Hashimoto, Yoshihide, Negishi, Jun, Saga, Hideki, Sasaki, Yoshihiro, Akiyoshi, Kazunari, Kimura, Tsuyoshi, Kishida, Akio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407827/
https://www.ncbi.nlm.nih.gov/pubmed/36012126
http://dx.doi.org/10.3390/ijms23168868
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author Kobayashi, Mako
Ishida, Naoki
Hashimoto, Yoshihide
Negishi, Jun
Saga, Hideki
Sasaki, Yoshihiro
Akiyoshi, Kazunari
Kimura, Tsuyoshi
Kishida, Akio
author_facet Kobayashi, Mako
Ishida, Naoki
Hashimoto, Yoshihide
Negishi, Jun
Saga, Hideki
Sasaki, Yoshihiro
Akiyoshi, Kazunari
Kimura, Tsuyoshi
Kishida, Akio
author_sort Kobayashi, Mako
collection PubMed
description Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.
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spelling pubmed-94078272022-08-26 Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues Kobayashi, Mako Ishida, Naoki Hashimoto, Yoshihide Negishi, Jun Saga, Hideki Sasaki, Yoshihiro Akiyoshi, Kazunari Kimura, Tsuyoshi Kishida, Akio Int J Mol Sci Article Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling. MDPI 2022-08-09 /pmc/articles/PMC9407827/ /pubmed/36012126 http://dx.doi.org/10.3390/ijms23168868 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kobayashi, Mako
Ishida, Naoki
Hashimoto, Yoshihide
Negishi, Jun
Saga, Hideki
Sasaki, Yoshihiro
Akiyoshi, Kazunari
Kimura, Tsuyoshi
Kishida, Akio
Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
title Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
title_full Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
title_fullStr Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
title_full_unstemmed Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
title_short Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
title_sort extraction and biological evaluation of matrix-bound nanovesicles (mbvs) from high-hydrostatic pressure-decellularized tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407827/
https://www.ncbi.nlm.nih.gov/pubmed/36012126
http://dx.doi.org/10.3390/ijms23168868
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