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Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery

Photoacoustic (PA) endoscopy has shown significant potential for clinical diagnosis and surgical guidance. Multimode fibres (MMFs) are becoming increasingly attractive for the development of miniature endoscopy probes owing to their ultrathin size, low cost and diffraction-limited spatial resolution...

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Detalles Bibliográficos
Autores principales: Zhao, Tianrui, Pham, Truc Thuy, Baker, Christian, Ma, Michelle T., Ourselin, Sebastien, Vercauteren, Tom, Zhang, Edward, Beard, Paul C., Xia, Wenfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optica Publishing Group 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408236/
https://www.ncbi.nlm.nih.gov/pubmed/36032566
http://dx.doi.org/10.1364/BOE.463057
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author Zhao, Tianrui
Pham, Truc Thuy
Baker, Christian
Ma, Michelle T.
Ourselin, Sebastien
Vercauteren, Tom
Zhang, Edward
Beard, Paul C.
Xia, Wenfeng
author_facet Zhao, Tianrui
Pham, Truc Thuy
Baker, Christian
Ma, Michelle T.
Ourselin, Sebastien
Vercauteren, Tom
Zhang, Edward
Beard, Paul C.
Xia, Wenfeng
author_sort Zhao, Tianrui
collection PubMed
description Photoacoustic (PA) endoscopy has shown significant potential for clinical diagnosis and surgical guidance. Multimode fibres (MMFs) are becoming increasingly attractive for the development of miniature endoscopy probes owing to their ultrathin size, low cost and diffraction-limited spatial resolution enabled by wavefront shaping. However, current MMF-based PA endomicroscopy probes are either limited by a bulky ultrasound detector or a low imaging speed that hindered their usability. In this work, we report the development of a highly miniaturised and high-speed PA endomicroscopy probe that is integrated within the cannula of a 20 gauge medical needle. This probe comprises a MMF for delivering the PA excitation light and a single-mode optical fibre with a plano-concave microresonator for ultrasound detection. Wavefront shaping with a digital micromirror device enabled rapid raster-scanning of a focused light spot at the distal end of the MMF for tissue interrogation. High-resolution PA imaging of mouse red blood cells covering an area 100 µm in diameter was achieved with the needle probe at ∼3 frames per second. Mosaicing imaging was performed after fibre characterisation by translating the needle probe to enlarge the field-of-view in real-time. The developed ultrathin PA endomicroscopy probe is promising for guiding minimally invasive surgery by providing functional, molecular and microstructural information of tissue in real-time.
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spelling pubmed-94082362022-08-26 Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery Zhao, Tianrui Pham, Truc Thuy Baker, Christian Ma, Michelle T. Ourselin, Sebastien Vercauteren, Tom Zhang, Edward Beard, Paul C. Xia, Wenfeng Biomed Opt Express Article Photoacoustic (PA) endoscopy has shown significant potential for clinical diagnosis and surgical guidance. Multimode fibres (MMFs) are becoming increasingly attractive for the development of miniature endoscopy probes owing to their ultrathin size, low cost and diffraction-limited spatial resolution enabled by wavefront shaping. However, current MMF-based PA endomicroscopy probes are either limited by a bulky ultrasound detector or a low imaging speed that hindered their usability. In this work, we report the development of a highly miniaturised and high-speed PA endomicroscopy probe that is integrated within the cannula of a 20 gauge medical needle. This probe comprises a MMF for delivering the PA excitation light and a single-mode optical fibre with a plano-concave microresonator for ultrasound detection. Wavefront shaping with a digital micromirror device enabled rapid raster-scanning of a focused light spot at the distal end of the MMF for tissue interrogation. High-resolution PA imaging of mouse red blood cells covering an area 100 µm in diameter was achieved with the needle probe at ∼3 frames per second. Mosaicing imaging was performed after fibre characterisation by translating the needle probe to enlarge the field-of-view in real-time. The developed ultrathin PA endomicroscopy probe is promising for guiding minimally invasive surgery by providing functional, molecular and microstructural information of tissue in real-time. Optica Publishing Group 2022-07-27 /pmc/articles/PMC9408236/ /pubmed/36032566 http://dx.doi.org/10.1364/BOE.463057 Text en Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhao, Tianrui
Pham, Truc Thuy
Baker, Christian
Ma, Michelle T.
Ourselin, Sebastien
Vercauteren, Tom
Zhang, Edward
Beard, Paul C.
Xia, Wenfeng
Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
title Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
title_full Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
title_fullStr Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
title_full_unstemmed Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
title_short Ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
title_sort ultrathin, high-speed, all-optical photoacoustic endomicroscopy probe for guiding minimally invasive surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408236/
https://www.ncbi.nlm.nih.gov/pubmed/36032566
http://dx.doi.org/10.1364/BOE.463057
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