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Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ
A critical issue to understanding how populations adapt to new selective pressures is the relative contribution of the initial standing genetic diversity versus that generated de novo. RNA viruses are an excellent model to study this question, as they form highly heterogeneous populations whose gene...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408265/ https://www.ncbi.nlm.nih.gov/pubmed/36012143 http://dx.doi.org/10.3390/ijms23168876 |
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author | Somovilla, Pilar Rodríguez-Moreno, Alicia Arribas, María Manrubia, Susanna Lázaro, Ester |
author_facet | Somovilla, Pilar Rodríguez-Moreno, Alicia Arribas, María Manrubia, Susanna Lázaro, Ester |
author_sort | Somovilla, Pilar |
collection | PubMed |
description | A critical issue to understanding how populations adapt to new selective pressures is the relative contribution of the initial standing genetic diversity versus that generated de novo. RNA viruses are an excellent model to study this question, as they form highly heterogeneous populations whose genetic diversity can be modulated by factors such as the number of generations, the size of population bottlenecks, or exposure to new environment conditions. In this work, we propagated at nonoptimal temperature (43 °C) two bacteriophage Qβ populations differing in their degree of heterogeneity. Deep sequencing analysis showed that, prior to the temperature change, the most heterogeneous population contained some low-frequency mutations that had previously been detected in the consensus sequences of other Qβ populations adapted to 43 °C. Evolved populations with origin in this ancestor reached similar growth rates, but the adaptive pathways depended on the frequency of these standing mutations and the transmission bottleneck size. In contrast, the growth rate achieved by populations with origin in the less heterogeneous ancestor did depend on the transmission bottleneck size. The conclusion is that viral diversification in a particular environment may lead to the emergence of mutants capable of accelerating adaptation when the environment changes. |
format | Online Article Text |
id | pubmed-9408265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94082652022-08-26 Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ Somovilla, Pilar Rodríguez-Moreno, Alicia Arribas, María Manrubia, Susanna Lázaro, Ester Int J Mol Sci Article A critical issue to understanding how populations adapt to new selective pressures is the relative contribution of the initial standing genetic diversity versus that generated de novo. RNA viruses are an excellent model to study this question, as they form highly heterogeneous populations whose genetic diversity can be modulated by factors such as the number of generations, the size of population bottlenecks, or exposure to new environment conditions. In this work, we propagated at nonoptimal temperature (43 °C) two bacteriophage Qβ populations differing in their degree of heterogeneity. Deep sequencing analysis showed that, prior to the temperature change, the most heterogeneous population contained some low-frequency mutations that had previously been detected in the consensus sequences of other Qβ populations adapted to 43 °C. Evolved populations with origin in this ancestor reached similar growth rates, but the adaptive pathways depended on the frequency of these standing mutations and the transmission bottleneck size. In contrast, the growth rate achieved by populations with origin in the less heterogeneous ancestor did depend on the transmission bottleneck size. The conclusion is that viral diversification in a particular environment may lead to the emergence of mutants capable of accelerating adaptation when the environment changes. MDPI 2022-08-09 /pmc/articles/PMC9408265/ /pubmed/36012143 http://dx.doi.org/10.3390/ijms23168876 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Somovilla, Pilar Rodríguez-Moreno, Alicia Arribas, María Manrubia, Susanna Lázaro, Ester Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ |
title | Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ |
title_full | Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ |
title_fullStr | Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ |
title_full_unstemmed | Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ |
title_short | Standing Genetic Diversity and Transmission Bottleneck Size Drive Adaptation in Bacteriophage Qβ |
title_sort | standing genetic diversity and transmission bottleneck size drive adaptation in bacteriophage qβ |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408265/ https://www.ncbi.nlm.nih.gov/pubmed/36012143 http://dx.doi.org/10.3390/ijms23168876 |
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