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Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.

The WUSCHEL-related homeobox (WOX) proteins are a class of transcription factors exclusive to plants. They can promote cell division or inhibit stem cell differentiation to regulate plant growth and development. However, the WOX transcription factor genes in the monocotyledon Dendrobium catenatum Li...

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Autores principales: Li, Hefan, Li, Cheng, Wang, Yuhua, Qin, Xiangshi, Meng, Lihua, Sun, Xudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408443/
https://www.ncbi.nlm.nih.gov/pubmed/36011392
http://dx.doi.org/10.3390/genes13081481
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author Li, Hefan
Li, Cheng
Wang, Yuhua
Qin, Xiangshi
Meng, Lihua
Sun, Xudong
author_facet Li, Hefan
Li, Cheng
Wang, Yuhua
Qin, Xiangshi
Meng, Lihua
Sun, Xudong
author_sort Li, Hefan
collection PubMed
description The WUSCHEL-related homeobox (WOX) proteins are a class of transcription factors exclusive to plants. They can promote cell division or inhibit stem cell differentiation to regulate plant growth and development. However, the WOX transcription factor genes in the monocotyledon Dendrobium catenatum Lindl. remain relatively uncharacterized. Specifically, the effects of phytohormones on their expression levels are unclear. In this study, we identified and analyzed 10 candidate DcaWOX transcription factor genes in D. catenatum. The DcaWOX family was divided into the modern/WUS, intermediate, and ancient clades. The subcellular localization analysis detected DcaWOX-GFP fusion proteins in the tobacco epidermal leaf cell nucleus. In DcaWOX, members of the WUS clade with the WUS-box motif can significantly activate the expression of TPL in vivo, while members of the intermediate and ancient clades cannot. The expression of the DcaWOX genes varied among the examined tissues. Moreover, the DcaWOX expression patterns were differentially affected by the phytohormone treatments, with differences detected even between homologs of the same gene. Furthermore, the gene expression patterns were consistent with the predicted cis-acting elements in the promoters. The above results suggest that DcaWOX may have an important role in its growth and development and resistance to stress. The results of this comprehensive investigation of the DcaWOX gene family provide the basis for future studies on the roles of WOX genes in D. catenatum.
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spelling pubmed-94084432022-08-26 Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl. Li, Hefan Li, Cheng Wang, Yuhua Qin, Xiangshi Meng, Lihua Sun, Xudong Genes (Basel) Article The WUSCHEL-related homeobox (WOX) proteins are a class of transcription factors exclusive to plants. They can promote cell division or inhibit stem cell differentiation to regulate plant growth and development. However, the WOX transcription factor genes in the monocotyledon Dendrobium catenatum Lindl. remain relatively uncharacterized. Specifically, the effects of phytohormones on their expression levels are unclear. In this study, we identified and analyzed 10 candidate DcaWOX transcription factor genes in D. catenatum. The DcaWOX family was divided into the modern/WUS, intermediate, and ancient clades. The subcellular localization analysis detected DcaWOX-GFP fusion proteins in the tobacco epidermal leaf cell nucleus. In DcaWOX, members of the WUS clade with the WUS-box motif can significantly activate the expression of TPL in vivo, while members of the intermediate and ancient clades cannot. The expression of the DcaWOX genes varied among the examined tissues. Moreover, the DcaWOX expression patterns were differentially affected by the phytohormone treatments, with differences detected even between homologs of the same gene. Furthermore, the gene expression patterns were consistent with the predicted cis-acting elements in the promoters. The above results suggest that DcaWOX may have an important role in its growth and development and resistance to stress. The results of this comprehensive investigation of the DcaWOX gene family provide the basis for future studies on the roles of WOX genes in D. catenatum. MDPI 2022-08-19 /pmc/articles/PMC9408443/ /pubmed/36011392 http://dx.doi.org/10.3390/genes13081481 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Hefan
Li, Cheng
Wang, Yuhua
Qin, Xiangshi
Meng, Lihua
Sun, Xudong
Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.
title Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.
title_full Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.
title_fullStr Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.
title_full_unstemmed Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.
title_short Genome-Wide Analysis of the WOX Transcription Factor Genes in Dendrobium catenatum Lindl.
title_sort genome-wide analysis of the wox transcription factor genes in dendrobium catenatum lindl.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408443/
https://www.ncbi.nlm.nih.gov/pubmed/36011392
http://dx.doi.org/10.3390/genes13081481
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