Cargando…

Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model

Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein,...

Descripción completa

Detalles Bibliográficos
Autores principales: Kua, Jonah Ee Hsiang, Siow, Chun Wei, Lim, Wee Keng, Masilamani, Jeyakumar, Tjin, Monica Suryana, Yeong, Joe, Lim, Tony Kiat Hon, Phan, Toan Thang, Chua, Alvin Wen Choong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408523/
https://www.ncbi.nlm.nih.gov/pubmed/36012184
http://dx.doi.org/10.3390/ijms23168918
_version_ 1784774622073323520
author Kua, Jonah Ee Hsiang
Siow, Chun Wei
Lim, Wee Keng
Masilamani, Jeyakumar
Tjin, Monica Suryana
Yeong, Joe
Lim, Tony Kiat Hon
Phan, Toan Thang
Chua, Alvin Wen Choong
author_facet Kua, Jonah Ee Hsiang
Siow, Chun Wei
Lim, Wee Keng
Masilamani, Jeyakumar
Tjin, Monica Suryana
Yeong, Joe
Lim, Tony Kiat Hon
Phan, Toan Thang
Chua, Alvin Wen Choong
author_sort Kua, Jonah Ee Hsiang
collection PubMed
description Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds.
format Online
Article
Text
id pubmed-9408523
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94085232022-08-26 Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model Kua, Jonah Ee Hsiang Siow, Chun Wei Lim, Wee Keng Masilamani, Jeyakumar Tjin, Monica Suryana Yeong, Joe Lim, Tony Kiat Hon Phan, Toan Thang Chua, Alvin Wen Choong Int J Mol Sci Article Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds. MDPI 2022-08-10 /pmc/articles/PMC9408523/ /pubmed/36012184 http://dx.doi.org/10.3390/ijms23168918 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kua, Jonah Ee Hsiang
Siow, Chun Wei
Lim, Wee Keng
Masilamani, Jeyakumar
Tjin, Monica Suryana
Yeong, Joe
Lim, Tony Kiat Hon
Phan, Toan Thang
Chua, Alvin Wen Choong
Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
title Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
title_full Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
title_fullStr Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
title_full_unstemmed Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
title_short Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
title_sort human umbilical cord lining-derived epithelial cells: a potential source of non-native epithelial cells that accelerate healing in a porcine cutaneous wound model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408523/
https://www.ncbi.nlm.nih.gov/pubmed/36012184
http://dx.doi.org/10.3390/ijms23168918
work_keys_str_mv AT kuajonaheehsiang humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT siowchunwei humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT limweekeng humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT masilamanijeyakumar humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT tjinmonicasuryana humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT yeongjoe humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT limtonykiathon humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT phantoanthang humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel
AT chuaalvinwenchoong humanumbilicalcordliningderivedepithelialcellsapotentialsourceofnonnativeepithelialcellsthatacceleratehealinginaporcinecutaneouswoundmodel