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Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model
Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408523/ https://www.ncbi.nlm.nih.gov/pubmed/36012184 http://dx.doi.org/10.3390/ijms23168918 |
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author | Kua, Jonah Ee Hsiang Siow, Chun Wei Lim, Wee Keng Masilamani, Jeyakumar Tjin, Monica Suryana Yeong, Joe Lim, Tony Kiat Hon Phan, Toan Thang Chua, Alvin Wen Choong |
author_facet | Kua, Jonah Ee Hsiang Siow, Chun Wei Lim, Wee Keng Masilamani, Jeyakumar Tjin, Monica Suryana Yeong, Joe Lim, Tony Kiat Hon Phan, Toan Thang Chua, Alvin Wen Choong |
author_sort | Kua, Jonah Ee Hsiang |
collection | PubMed |
description | Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds. |
format | Online Article Text |
id | pubmed-9408523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94085232022-08-26 Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model Kua, Jonah Ee Hsiang Siow, Chun Wei Lim, Wee Keng Masilamani, Jeyakumar Tjin, Monica Suryana Yeong, Joe Lim, Tony Kiat Hon Phan, Toan Thang Chua, Alvin Wen Choong Int J Mol Sci Article Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds. MDPI 2022-08-10 /pmc/articles/PMC9408523/ /pubmed/36012184 http://dx.doi.org/10.3390/ijms23168918 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kua, Jonah Ee Hsiang Siow, Chun Wei Lim, Wee Keng Masilamani, Jeyakumar Tjin, Monica Suryana Yeong, Joe Lim, Tony Kiat Hon Phan, Toan Thang Chua, Alvin Wen Choong Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model |
title | Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model |
title_full | Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model |
title_fullStr | Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model |
title_full_unstemmed | Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model |
title_short | Human Umbilical Cord Lining-Derived Epithelial Cells: A Potential Source of Non-Native Epithelial Cells That Accelerate Healing in a Porcine Cutaneous Wound Model |
title_sort | human umbilical cord lining-derived epithelial cells: a potential source of non-native epithelial cells that accelerate healing in a porcine cutaneous wound model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408523/ https://www.ncbi.nlm.nih.gov/pubmed/36012184 http://dx.doi.org/10.3390/ijms23168918 |
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