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The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis
Background: The detection rate of viral RNA in tick-borne encephalitis (TBE) is low and variable between studies, and its diagnostic/prognostic potential is not well defined. We attempted to detect RNA of TBE virus (TBEV) in body fluids of TBE patients. Methods: We studied 98 adults and 12 children...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408829/ https://www.ncbi.nlm.nih.gov/pubmed/36012596 http://dx.doi.org/10.3390/ijms23169332 |
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author | Grygorczuk, Sambor Dunaj-Małyszko, Justyna Czupryna, Piotr Sulik, Artur Toczyłowski, Kacper Siemieniako-Werszko, Agnieszka Żebrowska, Agnieszka Pancewicz, Sławomir Moniuszko-Malinowska, Anna |
author_facet | Grygorczuk, Sambor Dunaj-Małyszko, Justyna Czupryna, Piotr Sulik, Artur Toczyłowski, Kacper Siemieniako-Werszko, Agnieszka Żebrowska, Agnieszka Pancewicz, Sławomir Moniuszko-Malinowska, Anna |
author_sort | Grygorczuk, Sambor |
collection | PubMed |
description | Background: The detection rate of viral RNA in tick-borne encephalitis (TBE) is low and variable between studies, and its diagnostic/prognostic potential is not well defined. We attempted to detect RNA of TBE virus (TBEV) in body fluids of TBE patients. Methods: We studied 98 adults and 12 children with TBEV infection, stratified by the disease phase and presentation. EDTA blood and cerebrospinal fluid (CSF) samples were obtained upon hospital admission. RNA was extracted from freshly obtained plasma, concentrated leukocyte-enriched CSF, and whole blood samples, and real time PCR was performed with a Rotor-Gene Q thermocycler. Results: TBEV RNA was detected in (1) plasma of one (of the two studied) adult patients with an abortive infection, (2) plasma of two (of the two studied) adults in the peripheral phase of TBE, and (3) plasma and blood of an adult in the neurologic phase of TBE presenting as meningoencephalomyelitis. No CSF samples were TBEV RNA-positive. Conclusions: The detection of TBEV RNA in blood might be diagnostic in the peripheral phase of TBE. The lack of TBEV RNA in the CSF cellular fraction speaks against TBEV influx into the central nervous system with infiltrating leukocytes and is consistent with a relatively low intrathecal viral burden. |
format | Online Article Text |
id | pubmed-9408829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94088292022-08-26 The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis Grygorczuk, Sambor Dunaj-Małyszko, Justyna Czupryna, Piotr Sulik, Artur Toczyłowski, Kacper Siemieniako-Werszko, Agnieszka Żebrowska, Agnieszka Pancewicz, Sławomir Moniuszko-Malinowska, Anna Int J Mol Sci Article Background: The detection rate of viral RNA in tick-borne encephalitis (TBE) is low and variable between studies, and its diagnostic/prognostic potential is not well defined. We attempted to detect RNA of TBE virus (TBEV) in body fluids of TBE patients. Methods: We studied 98 adults and 12 children with TBEV infection, stratified by the disease phase and presentation. EDTA blood and cerebrospinal fluid (CSF) samples were obtained upon hospital admission. RNA was extracted from freshly obtained plasma, concentrated leukocyte-enriched CSF, and whole blood samples, and real time PCR was performed with a Rotor-Gene Q thermocycler. Results: TBEV RNA was detected in (1) plasma of one (of the two studied) adult patients with an abortive infection, (2) plasma of two (of the two studied) adults in the peripheral phase of TBE, and (3) plasma and blood of an adult in the neurologic phase of TBE presenting as meningoencephalomyelitis. No CSF samples were TBEV RNA-positive. Conclusions: The detection of TBEV RNA in blood might be diagnostic in the peripheral phase of TBE. The lack of TBEV RNA in the CSF cellular fraction speaks against TBEV influx into the central nervous system with infiltrating leukocytes and is consistent with a relatively low intrathecal viral burden. MDPI 2022-08-19 /pmc/articles/PMC9408829/ /pubmed/36012596 http://dx.doi.org/10.3390/ijms23169332 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grygorczuk, Sambor Dunaj-Małyszko, Justyna Czupryna, Piotr Sulik, Artur Toczyłowski, Kacper Siemieniako-Werszko, Agnieszka Żebrowska, Agnieszka Pancewicz, Sławomir Moniuszko-Malinowska, Anna The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis |
title | The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis |
title_full | The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis |
title_fullStr | The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis |
title_full_unstemmed | The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis |
title_short | The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis |
title_sort | detectability of the viral rna in blood and cerebrospinal fluid of patients with tick-borne encephalitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408829/ https://www.ncbi.nlm.nih.gov/pubmed/36012596 http://dx.doi.org/10.3390/ijms23169332 |
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