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Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase

Reversible protein phosphorylation mediated by protein kinases and phosphatases plays important roles in the regulation of leaf senescence. We previously reported that the senescence-associated leucine-rich repeat receptor-like kinase AtSARK autophosphorylates on both serine/threonine and tyrosine r...

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Autores principales: Guo, Zhaoxia, Mei, Yuanyuan, Wang, Dan, Xiao, Dong, Tang, Xianglin, Gong, Yaru, Xu, Xinxin, Wang, Ning Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408895/
https://www.ncbi.nlm.nih.gov/pubmed/36012141
http://dx.doi.org/10.3390/ijms23168873
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author Guo, Zhaoxia
Mei, Yuanyuan
Wang, Dan
Xiao, Dong
Tang, Xianglin
Gong, Yaru
Xu, Xinxin
Wang, Ning Ning
author_facet Guo, Zhaoxia
Mei, Yuanyuan
Wang, Dan
Xiao, Dong
Tang, Xianglin
Gong, Yaru
Xu, Xinxin
Wang, Ning Ning
author_sort Guo, Zhaoxia
collection PubMed
description Reversible protein phosphorylation mediated by protein kinases and phosphatases plays important roles in the regulation of leaf senescence. We previously reported that the senescence-associated leucine-rich repeat receptor-like kinase AtSARK autophosphorylates on both serine/threonine and tyrosine residues and functions as a positive regulator of Arabidopsis leaf senescence; the senescence-suppressed protein phosphatase SSPP interacts with and dephosphorylates the cytoplasmic domain of AtSARK, thereby negatively regulating leaf senescence. Here, 27 autophosphorylation residues of AtSARK were revealed by mass spectrometry analysis, and six of them, including two Ser, two Thr, and two Tyr residues, were further found to be important for the biological functions of AtSARK. All site-directed mutations of these six residues that resulted in decreased autophosphorylation level of AtSARK could significantly inhibit AtSARK-induced leaf senescence. In addition, mutations mimicking the dephosphorylation form of Ser384 (S384A) or the phosphorylation form of Tyr413 (Y413E) substantially reduced the interaction between AtSARK and SSPP. All results suggest that autophosphorylation of AtSARK is essential for its functions in promoting leaf senescence. The possible roles of S384 and Y413 residues in fine-tuning the interaction between AtSARK and SSPP are discussed herein.
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spelling pubmed-94088952022-08-26 Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase Guo, Zhaoxia Mei, Yuanyuan Wang, Dan Xiao, Dong Tang, Xianglin Gong, Yaru Xu, Xinxin Wang, Ning Ning Int J Mol Sci Article Reversible protein phosphorylation mediated by protein kinases and phosphatases plays important roles in the regulation of leaf senescence. We previously reported that the senescence-associated leucine-rich repeat receptor-like kinase AtSARK autophosphorylates on both serine/threonine and tyrosine residues and functions as a positive regulator of Arabidopsis leaf senescence; the senescence-suppressed protein phosphatase SSPP interacts with and dephosphorylates the cytoplasmic domain of AtSARK, thereby negatively regulating leaf senescence. Here, 27 autophosphorylation residues of AtSARK were revealed by mass spectrometry analysis, and six of them, including two Ser, two Thr, and two Tyr residues, were further found to be important for the biological functions of AtSARK. All site-directed mutations of these six residues that resulted in decreased autophosphorylation level of AtSARK could significantly inhibit AtSARK-induced leaf senescence. In addition, mutations mimicking the dephosphorylation form of Ser384 (S384A) or the phosphorylation form of Tyr413 (Y413E) substantially reduced the interaction between AtSARK and SSPP. All results suggest that autophosphorylation of AtSARK is essential for its functions in promoting leaf senescence. The possible roles of S384 and Y413 residues in fine-tuning the interaction between AtSARK and SSPP are discussed herein. MDPI 2022-08-09 /pmc/articles/PMC9408895/ /pubmed/36012141 http://dx.doi.org/10.3390/ijms23168873 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Zhaoxia
Mei, Yuanyuan
Wang, Dan
Xiao, Dong
Tang, Xianglin
Gong, Yaru
Xu, Xinxin
Wang, Ning Ning
Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase
title Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase
title_full Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase
title_fullStr Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase
title_full_unstemmed Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase
title_short Identification and Functional Analysis of Key Autophosphorylation Residues of Arabidopsis Senescence Associated Receptor-like Kinase
title_sort identification and functional analysis of key autophosphorylation residues of arabidopsis senescence associated receptor-like kinase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9408895/
https://www.ncbi.nlm.nih.gov/pubmed/36012141
http://dx.doi.org/10.3390/ijms23168873
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