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GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus

Vibrio alginolyticus is a widely distributed marine bacterium that is a threat to the aquaculture industry as well as human health. Evidence has revealed critical roles for small RNAs (sRNAs) in bacterial physiology and cellular processes by modulating gene expression post-transcriptionally. GcvB is...

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Autores principales: Liu, Bing, Fang, Jianxiang, Chen, Huizhen, Sun, Yuehong, Yang, Shan, Gao, Qian, Zhang, Ying, Chen, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409037/
https://www.ncbi.nlm.nih.gov/pubmed/36012664
http://dx.doi.org/10.3390/ijms23169399
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author Liu, Bing
Fang, Jianxiang
Chen, Huizhen
Sun, Yuehong
Yang, Shan
Gao, Qian
Zhang, Ying
Chen, Chang
author_facet Liu, Bing
Fang, Jianxiang
Chen, Huizhen
Sun, Yuehong
Yang, Shan
Gao, Qian
Zhang, Ying
Chen, Chang
author_sort Liu, Bing
collection PubMed
description Vibrio alginolyticus is a widely distributed marine bacterium that is a threat to the aquaculture industry as well as human health. Evidence has revealed critical roles for small RNAs (sRNAs) in bacterial physiology and cellular processes by modulating gene expression post-transcriptionally. GcvB is one of the most conserved sRNAs that is regarded as the master regulator of amino acid uptake and metabolism in a wide range of Gram-negative bacteria. However, little information about GcvB-mediated regulation in V. alginolyticus is available. Here we first characterized GcvB in V. alginolyticus ZJ-T and determined its regulon by integrated transcriptome and quantitative proteome analysis. Transcriptome analysis revealed 40 genes differentially expressed (DEGs) between wild-type ZJ-T and gcvB mutant ZJ-T-ΔgcvB, while proteome analysis identified 50 differentially expressed proteins (DEPs) between them, but only 4 of them displayed transcriptional differences, indicating that most DEPs are the result of post-transcriptional regulation of gcvB. Among the differently expressed proteins, 21 are supposed to be involved in amino acid biosynthesis and transport, and 11 are associated with type three secretion system (T3SS), suggesting that GcvB may play a role in the virulence besides amino acid metabolism. RNA-EMSA showed that Hfq binds to GcvB, which promotes its stability.
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spelling pubmed-94090372022-08-26 GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus Liu, Bing Fang, Jianxiang Chen, Huizhen Sun, Yuehong Yang, Shan Gao, Qian Zhang, Ying Chen, Chang Int J Mol Sci Article Vibrio alginolyticus is a widely distributed marine bacterium that is a threat to the aquaculture industry as well as human health. Evidence has revealed critical roles for small RNAs (sRNAs) in bacterial physiology and cellular processes by modulating gene expression post-transcriptionally. GcvB is one of the most conserved sRNAs that is regarded as the master regulator of amino acid uptake and metabolism in a wide range of Gram-negative bacteria. However, little information about GcvB-mediated regulation in V. alginolyticus is available. Here we first characterized GcvB in V. alginolyticus ZJ-T and determined its regulon by integrated transcriptome and quantitative proteome analysis. Transcriptome analysis revealed 40 genes differentially expressed (DEGs) between wild-type ZJ-T and gcvB mutant ZJ-T-ΔgcvB, while proteome analysis identified 50 differentially expressed proteins (DEPs) between them, but only 4 of them displayed transcriptional differences, indicating that most DEPs are the result of post-transcriptional regulation of gcvB. Among the differently expressed proteins, 21 are supposed to be involved in amino acid biosynthesis and transport, and 11 are associated with type three secretion system (T3SS), suggesting that GcvB may play a role in the virulence besides amino acid metabolism. RNA-EMSA showed that Hfq binds to GcvB, which promotes its stability. MDPI 2022-08-20 /pmc/articles/PMC9409037/ /pubmed/36012664 http://dx.doi.org/10.3390/ijms23169399 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Bing
Fang, Jianxiang
Chen, Huizhen
Sun, Yuehong
Yang, Shan
Gao, Qian
Zhang, Ying
Chen, Chang
GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
title GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
title_full GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
title_fullStr GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
title_full_unstemmed GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
title_short GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
title_sort gcvb regulon revealed by transcriptomic and proteomic analysis in vibrio alginolyticus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409037/
https://www.ncbi.nlm.nih.gov/pubmed/36012664
http://dx.doi.org/10.3390/ijms23169399
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